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  • 1
    Electronic Resource
    Electronic Resource
    A 195-kb cosmid walk encompassing the human Xq28 color vision pigment genes
    Amsterdam : Elsevier
    Genomics 6 (1990), S. 367-373 
    Details
    ISSN: 0888-7543
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0888-7543(90)90578-I
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  • 2
    Electronic Resource
    Electronic Resource
    Dynamics of concentrations and nutrient fluxes in the xylem of Ricinus communis– diurnal course, impact of nutrient availability and nutrient uptake (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 24 (2001), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Diurnal courses of nutrient transport in the xylem and their response to external availability of nutrients were studied. In soil culture, maximal concentrations in all analysed substances were observed during night-time. Over experimental periods of up to 20 d, concentrations of some ions increased, most by accumulation in the soil. Stringent nutrient conditions were established in a novel pressure chamber. An aeroponic nutrient delivery system inside allows the sampling of xylem sap from intact plants under full control of the nutrient conditions at the root. Analysis of xylem transport under these highly defined conditions established that (1) diurnal variations in concentrations and fluxes in the xylem are dominated by plant-internal processes; (2) concentrations of nutrients in the xylem sap are highly but specifically correlated with each other; (3) nitrate uptake and nitrate flux to the shoot are largely uncoupled; and (4) in continuous light, diurnal variations of xylem sap concentrations vanish. Step changes in nitrate concentrations of the nutrient solution established that (5) the concomitant increase in nitrate concentration and flux in the xylem is delayed by 2–3 h and is only transient. Diurnal variations of xylem sap composition and use of the new technique to elucidate xylem-transport mechanisms are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2001.00655.x
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  • 3
    Electronic Resource
    Electronic Resource
    Independent changes of inorganic pyrophosphate and the AT/ADP or UTP/UDP ratios in plant cell suspension cultures
    Amsterdam : Elsevier
    Plant Science 66 (1990), S. 59-63 
    Details
    ISSN: 0168-9452
    Keywords: cell cultures ; energy charge ; pyrophosphate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90169-O
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  • 4
    Electronic Resource
    Electronic Resource
    Zum nachweis isomerer phenylathanolamine
    Amsterdam : Elsevier
    Journal of Chromatography A 34 (1968), S. 264-265 
    Details
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0021-9673(68)80050-0
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  • 5
    Electronic Resource
    Electronic Resource
    High-level expression of functional cGMP-dependent protein kinase using the baculovirus system
    Amsterdam : Elsevier
    FEBS Letters 336 (1993), S. 163-167 
    Details
    ISSN: 0014-5793
    Keywords: Baculovirus expression ; cGMP-dependent protein kinase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(93)81632-A
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  • 6
    Electronic Resource
    Electronic Resource
    Aminolyse von 2-phenylaziridin
    Amsterdam : Elsevier
    Tetrahedron 27 (1971), S. 1033-1041 
    Details
    ISSN: 0040-4020
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0040-4020(01)92505-0
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  • 7
    Electronic Resource
    Electronic Resource
    Borkomplexe von oximen des phenacyl-t-amin-typs
    Amsterdam : Elsevier
    Tetrahedron 27 (1971), S. 221-230 
    Details
    ISSN: 0040-4020
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0040-4020(01)92412-3
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  • 8
    Electronic Resource
    Electronic Resource
    Expansion dynamics, metabolite composition and substance transfer of the primary root growth zone of Zea mays L. grown in different external nutrient availabilities (2003)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 26 (2003), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A combined analysis of growth and metabolite composition was performed in primary roots of Zea mays L. (Var. Alexander). The seedlings were hydroponically cultivated either in pure water or in complete nutrient solution. The overall root growth performance was similar in both treatments. Yet, digital image sequence processing methods resolved, that growth distribution and oscillatory movements within the growth zone depended strongly on external nutrient availability. Metabolite concentration profiles were similar in both treatments for most investigated metabolites, indicating a thorough mobilization of nutrient resources from the seed, but concentrations of glutamine, glutamic acid, NO3–, NH4+, malate and citrate showed pronounced differences between treatments. No diurnal variations in metabolite concentrations were found. Deposition rate profiles were in general more similar to relative elemental growth rate profiles than concentration profiles and were not affected by the treatment. Major ions were deposited maximally in front of the centre of growth activity, while greatest hexose deposition was found behind that. Relative to their abundance in the root growth zone, net rates of transfer from mature tissue were highest for sucrose, glutamic acid and aspartic acid, whereas glucose, fructose and most amino acids inversely showed high net rates of transfer out of the root growth zone, indicating a high catabolic rate for those substances there. NO3–, but not other nutrients, was transferred to a great extent from the root growth zone to the mature tissue in nutrient solution. Overall, the results show, that a careful analysis of growth dynamics allows quantifying and interpreting a number of important flux parameters in the growing organ and that the performance of the primary root does not depend strongly on external nutrient availability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.0016-8025.2003.01068.x
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  • 9
    Electronic Resource
    Electronic Resource
    Expression of cGMP-dependent protein kinase inEscherichia coli (1993)
    Springer
    Molecular and cellular biochemistry 127-128 (1993), S. 71-80 
    Details
    ISSN: 1573-4919
    Keywords: Cyclic GMP-dependent protein kinase ; Escherichia coli ; post-translational modification ; protein folding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Cyclic GMP-dependent protein kinase (cGMP kinase) is involved in the relaxation of smooth muscle. The enzyme has been cloned and expressed in eukaryotic cell lines but so far not in prokaryotic cells. Three vectors were constructed for the expression of Iα cGMP kinase inEscherichia coli. Transformation with the pET3a/cgk vector which uses the T7 RNA polymerase/promotor system resulted in efficient accumulation of cGMP kinase. Most of the protein was in an insoluble and catalytic inactive form. Various solubilization and refolding conditions did not yield an active enzyme. A small fraction of the cGMP kinase was present in the soluble cell extract. This fraction bound cGMP with high affinity but had no cGMP stimulated kinase activity. To prevent aggregation two additional vectors were constructed. (I) A bacterial leader sequence, which directs the export of proteins into the periplasmic space, was fused to the aminoterminus of the cGMP kinase. (II) A gram/gram+ shuttle vector for expression under the control of the tac promotor was used. Both constructs directed the synthesis of an isoluble and inactive cGMP kinase. These results suggest that large amounts of cGMP kinase can be expressed inE. coli, but mainly in an isoluble and inactive form. In contrast to eukaryotic cells, bacteria may lack systems for correct protein folding and/or posttranslational modification that are crucial for the productive folding and/or activation of cGMP kinase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01076758
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  • 10
    Electronic Resource
    Electronic Resource
    Prenatal diagnosis and management of fetal hydrocephaly and lissencephaly (1993)
    Springer
    Child's nervous system 9 (1993), S. 408-412 
    Details
    ISSN: 1433-0350
    Keywords: Walker-Warburg syndrome ; Miller-Dieker syndrome ; Lissencephaly ; Ventriculomegaly ; Spina bifida ; Cephalocentesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two cases of prenatal diagnosis of lissencephaly are presented in the context of a series of 118 cases of prenatally diagnosed hydrocephalus. Within this series there was one case of Walker-Warburg syndrome and another of Miller-Dieker syndrome. It is stressed that the cases reported here of ventriculomegaly diagnosed in utero show a very different outcome from those in published studies of fetal hydrocephalus which only deal with patients in whom the diagnosis was determined after birth. In those postnatal series there is a considerable selection bias, and the fate of the fetuses reported here was much worse than in postnatal series. Of the 118 fetuses 6 had fetal infections, 6 had chromosomal abnormalities, 26 had associated spina bifida, 64 fetuses had associated other anomalies, and only 28 had isolated hydrocephalus. Although it is difficult to determine the prognosis individually after prenatal diagnosis of ventriculomegaly, the data presented here may be helpful in counseling parents prenatally. The counseling should be performed with the collaboration of obstetricians, pediatricians, surgeons, and geneticists.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306194
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