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  • 1
    Electronic Resource
    Electronic Resource
    Comparative effects of a recombinant and a mutein type of granulocyte colony stimulating factor on the growth of Meth-A fibrosarcoma with 5-fluorouracil chemotherapy (1992)
    Springer
    Biotherapy 4 (1992), S. 81-86 
    Details
    ISSN: 1573-8280
    Keywords: G-CSF ; KW-2228 ; 5-FU
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study was designed to evaluate the effects of a recombinant human G-CSF (rhG-CSF) and a mutein G-CSF(KW-2228) on leucopenia and tumor growth in mice treated with 5-fluorouracil (5-FU). In normal mice, the number of leucocytes (white blood cell, WBC) reached the peak 12 hours after a single injection of either type of G-CSF and decreased to the normal level after 24 hours. Daily administration induced a continuous increase in the WBC count, however, administrations at intervals did not. Meth-A fibrosarcoma was subcutaneously inoculated into the backs of syngeneic BALB/c mice. The mice were treated with 5-FU alone or with G-CSFs. Chemotherapy with 5-FU alone resulted in leucopenia and an insignificant inhibition of tumor growth. The conjunctive administration of G-CSFs with 5-FU resulted in a significantly augmented inhibition of tumor growth, and leukopenia was not seen. This augmenting effect was more prominent with KW-2228. These results suggest that in 5-FU chemotherapy G-CSFs may be beneficial in restoring the number of leucocytes from leucopenic state and in augmenting the tumor inhibitory effect. Furthermore, KW-2228 may be more beneficial than the natural type rhG-CSF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02171752
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  • 2
    Electronic Resource
    Electronic Resource
    In vivo effects of human recombinant tumor necrosis factor alone and in combination with other biological response modifiers on human digestive organ cancer xenografts transplanted in nude mice (1991)
    Springer
    Biotherapy 3 (1991), S. 337-344 
    Details
    ISSN: 1573-8280
    Keywords: human tumor xenograft ; interferon (IFN) ; interleukin-2 (IL-2) ; nude mouse ; Picibanil (OK-432) ; tumor necrosis factor (TNF)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study was designed to evaluate the effect of rTNF alone or in combination with other BRMs on human digestive organ cancers. Six kinds of human digestive organ cancer xenografts (esophageal, stomach, colonic, pancreatic, bile duct, and liver cancers: EC-YO, GC-YN, CC-KK, PC-HN, BDC-SN and Li-7, respectively) were transplanted in nude mice, and rTNF was administered at 103, 5 × 103, or 104U/head directly into the tumor 3 times a week for 2 weeks. EC-YO was the most sensitive to rTNF, and intratumoral administration of rTNF at 103 U/head caused tumor regression. PC-HN, CC-KK and GC-YN were relatively sensitive to rTNF, and their growth was significantly inhibited by rTNF at 5 × 103 U/head, however, the tumors regrew after treatment. Li-7 and BDC-SN were resistant to rTNF. The effects of rTNF in combination with recombinant interferon-γ (rIFN-γ), recombinant interleukin-2 (rIL-2), or streptococcal preparation OK-432 were assessed in mice transplanted with GC-YN. All combinations of rTNF at 5 × 103 U/head and other BRMs were more effective than rTNF alone, and GC-YN tumors were completely regressed after treatment with a combination of rTNF and rIFN-γ or rTNF and OK-432. However in all cases, the combination of rTNF at 103 U/head and any other BRM did not improve the effect. Furthermore, the adverse effects of the combinations were more serious than those of rTNF alone. TNF may still be a useful cytokine, because it can induce the regression of tumors. However, for its clinical application, a method should be developed to reduce its side effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221326
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  • 3
    Electronic Resource
    Electronic Resource
    Primary large cell carcinoma of the submandibular gland (1999)
    Springer
    International journal of clinical oncology 4 (1999), S. 189-192 
    Details
    ISSN: 1437-7772
    Keywords: Key words Carcinoma of the submandibular gland ; Large cell carcinoma ; Radiation therapy ; Hyperthermia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A rare case of primary large cell carcinoma of the submandibular gland is reported. A 53-year-old man presented with a tumor in the left submandibular region extending to the supraclavicular fossa. After unsuccessful radiochemotherapy at another hospital, he was referred to our department for combined therapy of radiation, hyperthermia, and intraarterial anticancer drug infusion. Although the local tumor decreased in size after therapy, the patient died of respiratory insufficiency due to rapid progression of pulmonary metastases. Autopsy showed that tumor cells in both the submandibular gland and the lung were compatible with undifferentiated large cell carcinoma without tubular formation or laminar structure. The submandibular tumor was considered to be the primary site because cicatricial tissue surrounding the lesion suggested that had formed over a long period. The pulmonary lesions were considered to be metastases because necrosis and intravascular carcinoma cell embolism were noted. The above findings led to the final diagnosis of primary large cell carcinoma of the submandibular gland with pulmonary metastases. This condition has rarely been reported in the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101470050052
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  • 4
    Electronic Resource
    Electronic Resource
    Usefulness of preoperative low dose cisplatin treatment for advanced esophageal cancer (1992)
    Springer
    Surgery today 22 (1992), S. 409-415 
    Details
    ISSN: 1436-2813
    Keywords: esophageal cancer ; preoperative cisplatin treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to decrease the perioperative complications by preoperative cisplatin chemotherapy, the preoperative single administration of cisplatin (30 mg/m2) was performed weekly from one to six times in 36 consecutive patients with esophageal cancer classified as higher than Stage II. The survival curve of 17 patients in Stage III was significantly better (P〈0.05) than that of patients who had been treated without preoperative cisplatin treatment. In 3 of the 12 patients who had locally invasive cancer, either the main tumors or the metastatic lymph nodes, which had invaded the trachea or the left main bronchus, sufficiently receded, so that a curative esophagectomy became possible; 2 of them have survived over 33 months while 1 died of pneumonia 33 months after surgery. The number of perioperative complications was minimal, and thus, we consider that the postoperative use of cisplatin and fluorouracil is indicated in patients in whom a histological response is noted in the resected specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00308789
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of amplified sequences in mammalian DNA by in-gel renaturation and SINE hybridization (1986)
    Springer
    Somatic cell and molecular genetics 12 (1986), S. 611-623 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The presence of amplified sequences in mammalian DNA can be determined by in-gel renaturation of labeled restriction digests of total genomic DNA, provided that such sequences comprise at least 20–30 copies per haploid human or hamster genome or 40–50 copies per mouse genome. To detect amplified DNA in mouse cells at a lower level of amplification, a new procedure has been developed. This procedure combines in-gel DNA renaturation with Southern hybridization using a cloned probe containing a short interspersed repeated element (SINE). Using mouse cell lines containing amplified dihydrofolate reductase (DHFR)or c-Ki-rasgenes as a model system, and the cloned B2 repeated sequence as a SINE probe, we have shown that this technique can detect gene amplification at a level as low as 10–15 copies of amplified DNA per haploid mouse genome. In-gel renaturation-SINE hybridization was used to assay for DNA amplification in different tissues and in different mouse strains. No tissue-specific DNA amplification has been detected, but comparison of B2-containing repeated fragments between three inbred mouse lines revealed strain-specific polymorphism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01671946
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  • 6
    Electronic Resource
    Electronic Resource
    Immunochemical characteristics of a novel cell death receptor and a decoy receptor on granulosa cells of porcine ovarian follicles (2000)
    Springer
    Cytotechnology 33 (2000), S. 189-201 
    Details
    ISSN: 1573-0778
    Keywords: apoptosis ; cell death receptor ; decoyreceptor ; granulosa cell ; porcine ovary
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Previously, we prepared an IgM monoclonal antibody(PFG-1) which specifically recognized a cell-membraneglycoprotein (PFG-1 antigen; 55 kD, pI 5.9),immunohistochemically reacted with granulosa cells ofhealthy follicles but not of atretic follicles, andinduced granulosa cell apoptosis. In the presentstudy, an IgM monoclonal antibody (PFG-3) capable ofinducing granulosa cell apoptosis and an IgGmonoclonal antibody (PFG-4) not capable of inducingapoptosis were produced against granulosa cellsprepared from healthy antral follicles of porcineovaries. Two-dimensional Western blotting analysisrevealed that PFG-3 specifically recognized twocell-membrane proteins (named PFG-3-1 andPFG-3-2/PFG-1 antigens; 42 kD, pI 5.2 and 55 kD, pI5.9, respectively) of healthy granulosa cells, andthat PFG-4 recognized the same two cell-membraneproteins. In atretic granulosa cells, PFG-3-2/PFG-1antigen disappeared. Immunochemical reactions of theseantibodies were only detected in follicular granulosacells but not any other ovarian tissues or organs.PFG-3 and PFG-4 immunohistochemically reacted withgranulosa cells of healthy and atretic follicles. Whenthe isolated granulosa cells prepared from healthyfollicles were cultured in medium containing PFG-3,the cells underwent apoptosis, and co-incubation withPFG-4 inhibited PFG-3-inducible apoptosis. Theseobservations suggested that PFG-3-2/PFG-1 antigen isa novel cell death receptor which is different fromthe apoptosis-mediating receptors (Fas/Apo-1/CD95 orTNF receptor), and that PFG-3-1 antigen may act as adecoy receptor and inhibit apoptotic signal transmission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008146119761
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