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  • 1
    Electronic Resource
    Electronic Resource
    Nonfunctioning Adrenal Cortical Cancer and Analysis of the Steroidogenic Activity of the Tumor: A Case Report (1998)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of urology 5 (1998), S. 0 
    Details
    ISSN: 1442-2042
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: An adrenocortical cancer was detected by a CT scan in a 37-year-old woman, which did not have an excessive secretion of steroids or catecholamines. The tumor was enhanced inhomogeneously by the bolus injection of contrast medium, and magnetic resonance imaging (MRI) showed that the tumor was hypointense compared to the liver on a T1-weighted image and was diffusely hyperintense on a T2-weighted image. Histologically, the tumor consisted predominantly of compact cells with marked cellular and nuclear pleomorphism, but no capsular or vascular invasion were observed. An analysis of the steroidogenic activity of the tumor revealed that the activity of mitochondrial P450c11j8 was extremely reduced in the tumor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1442-2042.1998.tb00270.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 (HHV-8) in a primary effusion lymphoma cell line treated with tetradecanoyl phorbol acetate (TPA) (1999)
    Springer
    Medical electron microscopy 32 (1999), S. 94-99 
    Details
    ISSN: 1437-773X
    Keywords: Key words KSHV ; HHV-8 ; TPA ; Ultrastructure ; Primary effusion lymphoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 (HHV-8) has not yet been fully elucidated, although some findings have been reported using primary effusion lymphoma (PEL) cell lines, KS-1, harboring no Epstein–Barr virus (EBV) coinfection. In the present study, detailed fine structural examination of KSHV/HHV-8 was performed after stimulation of the PEL-derived cell line KS-1 with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in vitro. While unstimulated KS-1 cells contained a small number of intranuclear virus particles associated with no extracellular mature particles, KS-1 cells stimulated with TPA produced many extracellular mature particles as well as intranuclear particles, in addition to interesting tubulo-reticular structures and aggregated tubular structures in vesicles. The induced intranuclear particles were empty, doughnut shaped, and dense cored, with outer and inner diameters of 100–110 nm and 60–70 nm, respectively. Dense-cored extracellular mature particles were 150–160 nm in diameter, and some contained doughnut-shaped cores, together with a few megaloviruses, 260 nm in outer diameter. These findings indicate that KS-1 cells treated with TPA can produce extracellular mature particles as well as intranuclear particles, which were proven to be KSHV/HHV-8.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007950050014
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  • 3
    Electronic Resource
    Electronic Resource
    Sun-exposure- and aging-dependent p53 protein accumulation results in growth advantage for tumour cells in carcinogenesis of nonmelanocytic skin cancer (1999)
    Springer
    Virchows Archiv 434 (1999), S. 193-199 
    Details
    ISSN: 1432-2307
    Keywords: Key words Skin cancer ; p53 ; Differentiation ; Sun exposure ; Ageing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Three hundred and sixteen patients with nonmelanocytic skin cancer, including 46 cases of Bowen’s disease (BOD), 134 cases of squamous cell carcinoma (SCC), and 136 cases of basal cell carcinoma (BCC), were examined immunohistochemically using monoclonal antibody DO-7 to assess p53 protein accumulation related to sun exposure and ageing, and growth and differentiation of skin cancer and its precursors. The rates of p53 immunostaining of BOD, SCC and BCC were 80.4%, 76.1% and 70.6%, respectively. p53-positive cells were present not only in cancer nests, but also in dysplastic and even morphologically normal epidermis adjoining cancers. Sun exposure was statistically correlated with the p53 immunostaining scores in morphologically normal epidermis of the three skin cancers and in cancer nests of SCC and BCC. The positivity and score of p53 protein often differed significantly among the three types of cancer, especially in regions of dysplasia. Interestingly, differentiation of SCC was correlated with individual p53 scores for dysplasia and cancer nests, especially for dysplasia. BOD, as the precursor of SCC, demonstrated the strongest p53 expression. Furthermore, 12.3% cases with p53 negative cancer nests showed p53-positive reaction in dysplasia and in morphologically normal epidermis. It seems that the accumulation of p53 protein plays a part in precancerous lesions and in the genesis of more highly differentiated types of skin cancer and affects mainly the growth of tumour cells rather than their differentiation. For BCC, however, age was significantly related to p53 expression. Our findings suggest that overexpression of p53 in normal skin and cancer nests of SCC and BCC is significantly related to sun exposure, that the expression of p53 in BCC is an age-dependent process, and that the early accumulation of p53 protein may be a useful predictor for the detection of nonmelanocytic skin cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280050327
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution of S-100 protein-positive dendritic cells and expression of HLA-DR antigen in transitional cell carcinoma of the urinary bladder in relation to tumour progression and prognosis (1993)
    Springer
    Virchows Archiv 422 (1993), S. 351-355 
    Details
    ISSN: 1432-2307
    Keywords: Bladder cancer ; S-100 protein ; HLA-DR/dendritic cell ; Multivariate analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The distribution of S-100 protein positive dendritic cells (S100-DCs) in cancer nests and the expression of HLA-DR antigen on cancer cells in 90 patients with transitional cell carcinoma of the urinary bladder were studied immunohistochemically. A dense infiltrate of S100-DCs (more than 10 S100-DCs/high power field) was detected in 47 out of 90 cases, while in the remaining tumours the infiltrate was sparse. HLA-DR positive cancer cells (DR-CCs) were detected in 24 cases, including 16 with dense DR-CCs (more than 100 DR-CCs/high power field); no expression was observed in the remaining tumours. In terms of the numbers of S100-DCs infiltrating the following statistically significant differences were observed: tumour grading G1〉G3, depth of penetration pT0〉pT3; (p〈0.05), G2〉G3, lymphatic invasion-〉+ and venous invasion -〉+; (p〈0.01). A multivariate analysis demonstrated that the most important factor affecting prognosis was distant organ and/or lymph node metastasis (p〈0.01) the number of S100DCs, with a hazard ratio (HR) of 0.26 (p〈0.01), and the number of DR-CCs with HR of 0.18 (p〈0.05); these were statistically significant. S100-DCs and DR-CCs may be regarded as independent prognostic factors of tumour growth and progression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01605452
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  • 5
    Electronic Resource
    Electronic Resource
    Immunohistochemical and ultrastructural studies of intraluminal crystalloids in human prostatic carcinomas (1992)
    Springer
    Virchows Archiv 421 (1992), S. 421-425 
    Details
    ISSN: 1432-2307
    Keywords: Crystalloid ; Prostatic cancer ; Epithelial membrane antigen ; Phosphotungstic acid hematoxylin stain ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intraluminal crystalloids (ICr) observed in 19 cases of incidental or invasive human prostatic carcinoma (PCa) and in a case of benign prostatic hyperplasia were examined extensively by immunohistochemistry and electron microscopy. They were brilliantly eosinophilic with haematoxylin and eosin, manifesting needlelike, triangular, rectangular, hexagonal and irregular lump-like in shape. They were strongly positive, dark blue, with phosphotungstic acid -haematoxylin (PTAH) stain in all cases examined. Among the human antibodies tested, epithelial membrane antigen (EMA) gave specifically positive immunostainability with ICr in all cases. Annual ring-like lamellar or concentric structures were detected by electron microscopy. Positive staining of ICr with PTAH and anti-EMA antibody is very useful as a diagnostic marker for PCa in human prostatic tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606915
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  • 6
    Electronic Resource
    Electronic Resource
    Immunohistochemical characterization of HLA-DR-antigen positive dendritic cells in phaeochromocytomas and paragangliomas as a prognostic marker (1991)
    Springer
    Virchows Archiv 418 (1991), S. 33-39 
    Details
    ISSN: 1432-2307
    Keywords: HLA-DR ; Phaeochromocytomas ; Paragangliomas ; Immunohistochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Twelve cases of phaeochromocytoma (PCC) and four cases of paraganglioma (PGG) were studied by immunohistochemistry and immunoelectron microscopy in order to demonstrate HLA-DR (Ia)-antigen-positive dendritic cells (IaDCs). Dense infiltration of IaDCs was detected in the majority of PCCs revealing high urinary or serum catecholamine levels, but in aggressively growing PCCs, a familial PCC and all PGGs, few IaDCs were demonstrated. Interestingly, these IaDCs were negative for S-100 protein. Although S-100-protein-positive sustentacular-like cells (SCs), morphologically similar to IaDCs, were also present, these were clearly distinguished from IaDCs by our double immunostaining method. Ultrastructurally, IaDCs had smooth or slightly indented nuclei and contained a moderate amount of endoplasmic reticulum, small mitochondria and vacuoles, extending elongated cytoplasmic processes. These results suggest that determination of the quantity of IaDCs is a highly effective method of assessing the character of PCCs, in particular, their prognosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01600242
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  • 7
    Electronic Resource
    Electronic Resource
    Melanosis of the prostate: an immunohistochemical study (1998)
    Springer
    Medical molecular morphology 31 (1998), S. 38-41 
    Details
    ISSN: 1860-1499
    Keywords: Melanosis ; Prostate ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report here a case of prostatic melanosis examined immunohistochemically. The patient was a 70-year-old man with prostatic hyperplasia. Six foci with brown pigmentation were found, not only in stromal cells but also in glandular epithelial cells among many pieces of prostatic tissue. Immunohistochemically, only pigment-laden stromal cells were obviously positive with antibodies to S-100 protein and melanoma (HMB45), but not for CD57, while epithelial cells were only positive for CD57 after depigmentation with bleaching. This is the first report demonstrating immunopositivity of stromal cells for antimelanoma antibody (HMB45), which can label reactive or dysplastic but not normal melanocytes. These results clearly demonstrated that pigment-laden stromal cells are neuroectodermal in origin, and probably reactive or dysplastic but not normal melanocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01547947
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  • 8
    Electronic Resource
    Electronic Resource
    Immunohistochemical and ultrastructural studies of thyroid tissue from a patient with Basedow's disease and immunopositive for human T-lymphotropic virus type I (HTLV-I) (1994)
    Springer
    Medical molecular morphology 27 (1994), S. 136-144 
    Details
    ISSN: 1860-1499
    Keywords: Human T-lymphotropic virus type I (HTLV-I) ; Ultrastructure ; Basedow's disease ; Lymphocyte ; Thyroid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Thyroid tissue from a patient with Basedow's disease and immunopositive for HTLV-I was examined. Histopathological and immunohistochemical studies revealed that follicular-epithelial cells (FEC) had papillary projections reaching into the follicular lumina which were associated with diffuse T, but not B, lymphocyte infiltration and occasionally formed aggregations intermingled with T and B lymphocytes. Ultrastructurally, infiltrated lymphocytes possessing round or irregularly indented nuclei were detected in the capillary lumen, stroma and, also, FEC layers. The lymphocytes extended irregular processes, attached to long thin processes of FEC and infiltrated into FEC layers. Cocultivation of minced thyroid tissue with normal lymphocytes resulted in the establishment of a type-C virus-producing T-cell line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02348178
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  • 9
    Electronic Resource
    Electronic Resource
    Immunoelectron microscopy study on the expression of T-cell markers in human T-lymphotropic virus type II-(HTLV-II-) producing cells (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 125-130 
    Details
    ISSN: 1860-1499
    Keywords: Human T-lymphotropic virus type II ; T-cell receptor ; Lymphocyte marker ; Immunoelectron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An immunoelectron microscopy study of human T-lymphotropic virus (HLTV) type IIa- and type IIb-producing cells was performed to clarify lymphocyte marker expression including that of T-cell receptor (TCR). Although an immune reaction with TCR antibody β-chain was detected in the rough endoplasmic reticulum, but not the plasma membrane (PM), staining for TCR α-chain antibody was mostly negative in HLTV-IIa- and IIb-producing cells. Moreover. TCR α/β antibody yielded no positive reaction in HLTV-IIa- and-IIb-producing cells, including PM. Both the PM and nuclear membrane in HLTV-IIa- and-IIb-producing cells were positive for CD3; CD25 and HLA-DR were positive for the PM of HLTV-IIa- and-IIb-producing cells. These findings suggest that HLTV-IIa- and-IIb-producing cells are activated T cells expressing CD3, CD25, and HLA-DR antigens, but are immune deficient for antigen recognition because of defective expression of TCR-CD3 complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545313
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