ISSN:
1420-908X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We investigated the capacity ofStaphylococcus aureus Cowan I (Cowan Staph A+) andStaphylococcus aureus Wood 46 (Wood Staph A−) to induce histamine release from human basophilsin vitro. Cowan Staph A+ (3 × 106 to 3 × 108/ml), which synthesizes protein A (Staph A), stimulated the release of histamine from basophils, whereas Wood Staph A− (3×106 to 3×109/ml), which does not synthesize Staph A, did not induce histamine secretion. Soluble Staph A (10−3 to 10 μg/ml) also induced histamine secretion from human basophils, Hyperiodination of Staph A, which destroys over 90% of the original Fc reactivity without altering the Fab binding site, did not alter this protein's ability to induce histamine release. The stimulating effect of Staph A was suppressed by preincubation with human polyclonal IgG and a human monoclonal IgM, which have F(ab′)-Staph A reactivity. In contrast, rabbit IgG and a human monoclonal IgM possessing only Fe-Staph A reactivity did not inhibit Staph A activity. Preincubation with Staph A or Cowan Staph A+ resulted in complete cross-desensitization to a subsequent challenge with homologous and heterologous stimuli. These results indicate that Staph A and Cowan Staph A+ activate human basophils by interacting with the F(ab′)2 region of IgE and/or IgG present on the cell surface.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01982873
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