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  • 1
    Electronic Resource
    Electronic Resource
    Amalgam marginal quality assessment: a comparison of seven methods (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral rehabilitation 24 (1997), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: summary Black's class I classic cavities were cut in 120 sound extracted human premolars and restored with one of two silver amalgams, six different base combinations, with or without cavity varnish and then aged for 3 months or 1 year. Thereafter, the restored teeth were subjected to a bacterial or acid broth in vitro carious challenge for 36 days. Resin cast impressions were made of the restoration margin of each specimen before and after the carious challenge giving a total of 240 replicas. The replicas were examined in a scanning electron microscope (SEM) and the margins subjected to seven marginal assessment procedures: (i) ranking using micrographs of the margin; (ii) percentage length of marginal discrepancy; (iii) Mjör assessment using six groups; (iv) number of margin segments showing a discrepancy; (v) greatest gap size; (vi) ranking based firstly on gap size and then number of margin segments showing a discrepancy; and (vii) ranking based firstly on gap size and then percentage length of marginal discrepancy. The data from procedures (i), (ii), (vi) and (vii) were examined using one-way ANOVA; a linear logistic statistical analysis examined data from procedures (iii)–(v). Statistical significance was chosen at P〈0.05. It was found that procedure (vii) was able to discriminate between variables to a greater degree than all other procedures. It is recommended that a ranking system based on greatest gap size and percentage length of marginal discrepancy be used to assess marginal quality of amalgam restorations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2842.1997.tb00364.x
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  • 2
    Electronic Resource
    Electronic Resource
    Studies related to reaction of supporting soft tissue to denture wear: the histological response of vervet monkey oral epithelium to a -80 mmHg vacuum (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral rehabilitation 17 (1990), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The histological response of vervet monkey oral epithelium to a negative force similar to that experienced under dentures was investigated. Using impression trays with adherent impressions linked to a vacuum pump, the epithelia of the hard palate, attached gingiva and alveolar mucosa of 16 monkeys were subjected to a continuous vacuum of -80 mmHg, and then fixed by perfusion and immersion while in situ. After processing for light microscopy, sections were measured to obtain the rete peg length, supra-papillary width, epithelial width and basal, spinous and superficial cell density 700 μm−2. Mean values and standard deviations were calculated for each measurement and, using Student's t-test, these data were compared with results obtained for normal tissue from nine additional monkeys. The vacuum caused an increase in epithelial width in the palate and attached gingiva, and a decrease in epithelial width in the alveolar mucosa. The cell density 700 μm−2 decreased significantly in all layers of the palate, but increased in the basal layer of the attached gingiva and the basal and superficial layers of the alveolar mucosa. The alveolar mucosa within 0.5 mm of the mucogingival junction showed a variable response. This study demonstrates that a vacuum of -80 mmHg modifies the structure of the oral epithelium, and this response is directly related to the functional demands of the tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2842.1990.tb01430.x
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  • 3
    Electronic Resource
    Electronic Resource
    The ultrastructural response to loading of the oral mucosa of the vervet monkey (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 18 (1983), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The ultrastructural changes occurring within the epithelium of the palate, attached gingiva, tongue, buccal mucosa, and alveolar mucosa of the vervet monkey when a mechanical load was applied to the tissues was examined. The effect of the load was assessed in terms of changes in epithelial cell and nuclear shape, intracytoplasmic structures, cell membrane interdigitation, intercellular space, and changes in the shape of non-keratinocytes found within the epithelium. Loading produced a flattening of cells and nuclei throughout the epithelium. Intercellular space was reduced, cell membranes were flattened or else underwent increased interdigitation, tonofilaments became functionally oriented, and cytoplasmic vacuolation took place. The mitochondria appeared unchanged on loading. These changes were more marked in the nonkeratinized buccal and alveolar mucosa than in the keratinized palate, tongue, and attached gingiva. A histometric analysis which was carried out on the normal and loaded attached gingiva and alveolar mucosa revealed that the cells and nuclei of both tissue types underwent a significant change in width when loaded. Loading produced no significant change in the diameter of mitochondria in the basal and spinous layers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1983.tb00385.x
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  • 4
    Electronic Resource
    Electronic Resource
    Primary fixation of vervet monkey oral epithelium for ultrastructural investigation-SEM (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 18 (1989), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Vervet monkey attached gingiva and alveolar mucosa was used to investigate the effect of primary fixative composition and osmolarity on the scanning electron microscope appearance and epithelial cell surface feature density. Primary fixation was obtained using 12 different fixatives with osmolarities varying between 320–2010mOsm followed by further standard SEM processing procedures. All primary fixatives investigated produced acceptably fixed oral epithelium for SEM study, showing all the morphologic features characteristic of either keratinized or non-keratinized oral tissue. Point counting revealed that the density of microvilli of attached gingiva epithelial cells when fixed at 2010mOsm was 72±8% of the cell surface area. This decreased to 40±5% when fixed at 320mOsm. Similarly the microplication density of the alveolar mucosa epithelial cells decreased from 70±5% at 2010mOsm to 43±7% at 320mOsm. Both these differences proved to be highly significant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1989.tb00766.x
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  • 5
    Electronic Resource
    Electronic Resource
    A quantitative electron microscope study of desmosomes and hemi-desmosomes in vervet monkey oral mucosa (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 18 (1983), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The distribution and size of hemidesmosomes on the basal cell membrane adjacent to the basement lamina and desmosomes on the spinous cell membranes were compared in the attached gingiva, hard palate, tongue, alveolar mucosa, and buccal mucosa of the vervet monkey. The lengths of the sectioned cell membranes and pooled desmosome lengths were measured on photomicrographs of basal and spinous cells printed at standard magnification. The mean desmosomal length, interdesmosomal distance, and percentage cell membrane occupied by desmosomes was calculated. It was found that the percentage cell membrane occupied by hemidesmosomes was significantly higher in the basal cells of the attached gingiva and palate than in the alveolar mucosa, buccal mucosa, and tongue. In the spinous cell layer the percentage cell membrane occupied by desmosomes and the desmosome length was significantly higher in the tongue, attached gingiva, and hard palate than in the alveolar mucosa and buccal mucosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1983.tb00395.x
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    The use of extraction replicas in scanning electron microscopy for the elemental analysis of marginal seals (1987)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 21 (1987), S. 955-964 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Elemental analysis of marginal seal material on 20 bulk samples of occlusally restored teeth have shown that, generally, more elements were detected in the seal material analyzed on the bulk restoration or fractured tooth surfaces than when such material was extracted using a nitrocellulose replica prior to analysis. No mercury was detected in any of the extracted seal material and only three of the replica specimens contained silver. This implies that the technique of using extraction replicas to remove material from the amalgam-tooth interface for subsequent x-ray microanalysis can provide an accurate elemental composition. The details of fabrication and the advantages of using high resolution nitrocellulose replicas for extracting marginal seal material from teeth for subsequent x-ray microanalysis are described.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820210802
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