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  • 1
    Electronic Resource
    Electronic Resource
    Anaplasma marginale: failure of sera from immune cattle to confer protection in passive-transfer experiments (1992)
    Springer
    Parasitology research 78 (1992), S. 410-415 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract High levels of immunity toAnaplasma marginale were induced in cattle either by vaccination using sonically disruptedA. marginale-infected erythrocytes or by repeated infection with different strains of the rickettsia. In both instances, high levels of anti-A. marginale antibody were detected in the sera of the immune cattle by immunoblotting. Serum from one animal that had been made immune by repeated infection was transferred intravenously toA. marginale-susceptible calves (three non-splenectomised and two splenectomised) undergoing initialA. marginale infection at serum doses of 2–10 ml/kg. Neither the course nor the outcome of infection as indicated by the parasite levels attained or the level of anaemia induced was altered in the calves that received the immune serum relative to the course or outcome of infection in control calves (two non-splenectomised and two splenectomised) that received serum from anA. marginale-naive donor animal. In a similar experiment, a pool of sera from four steers that had been vaccinated with sonically disruptedA. marginale initial bodies was transfused into two intactA. marginale-susceptible calves during the early stage ofA. marginale infection at a dose of 10 ml/kg. No difference was observed in the course or outcome of infection in these calves relative to the course or outcome of infection in the two non-splenectomised calves that were transfused with non-immune serum. The failure of serum obtained from animals that had been made immune toA. marginale either by infection or by vaccination with disrupted initial bodies to confer any protection against infection following its transfer into splenectomised or non-splenectomised naive calves indicates that antibody per se is not a significant factor in immunity to this parasite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931697
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Peripheral blood lymphocyte proliferative responses in cattle infected with or vaccinated against Anaplasma marginale (1996)
    Springer
    Parasitology research 82 (1996), S. 551-562 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  An assay was developed for measurement of the peripheral blood lymphocyte proliferative response (PBLPR) in cattle infected with or immunised against Anaplasma marginale. PBLPR was not evident in all cattle that had recovered from A. marginale infection. However, A. marginale-sensitised lymphocytes were detected in the spleens of all immune cattle tested in the absence of detectable PBLPR. During the course of initial infection, cattle exhibited detectable PBLPR for a period corresponding with and up to 2 weeks after patent parasitaemia, followed by a second, usually larger peak in PBLPR corresponding to the time of sub-clinical relapse of cattle. Analysis of the PBLPR of A. marginale chronically infected cattle demonstrated highly variable PBLPR between individuals and over time. A positive PBLPR was induced in cattle by vaccination using a crude A. marginale antigen preparation. The PBLPR of vaccinated cattle subsequently infected with A. marginale was markedly different from that of naive cattle, with reduced PBLPR being associated with the onset of parasitaemia. The antigen used in the PBLPR assay was inactivated by proteolysis. Proteolysis also abolished immunity that had been induced in cattle vaccinated using the antigen preparation. A. marginale-sensitised PBL did not proliferate in response to antigen from the heterologous species A. centrale. A. centrale-sensitised PBL, however, responded to A. marginale antigen. Interferon-γ (IFN-γ) was detected in PBLPR-assay supernatants and was associated with a strong PBLPR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050161
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen (1990)
    Springer
    Parasitology research 76 (1990), S. 648-652 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinantB. bovis antigen expressed inEscherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931081
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    Paper (German National Licenses)
    Fulltext
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