ISSN:
1432-1955
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinantB. bovis antigen expressed inEscherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00931081