ISSN:
1615-6102
Keywords:
ATPases
;
Cation content
;
Light microscopic cytochemistry
;
Mg2+-K+ ATPase
;
Plasma membrane
;
Transfer cells
;
X-ray microanalysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A detailed comparison of Mg2+-K+ ATPase activity and cation fluxes in terminal buds, inhibited buds or buds released from apical dominance was carried out. Light microscope observations indicate intense reaction at the plasmalemma of stelar cells, pericyclic, phloem and xylem transfer cells at nodes along the main rhizome. In intact plants, with the exception of pericyclic cells, bud branches show no ATPase activity. Excision of the terminal bud results in rapid (within 5–15 minutes) stimulation of ATPase activity at nodes and all bud axes. As the subapical bud gains precedence, ATPase stimulation ceases and returns to its initial level in the older median and basal buds. Enzyme activation is kinetically correlated with K+ flux. X-ray microanalysis confirms that K+ accumulates in the stele at the node and the bud branch with the same lag period. This data increases the evidence for close association between ATP (Sossountzov et al. 1982), ATPase activity and K+ flux. The kinetics strengthen the impression that these factors may be involved very early in bud outgrowth regulation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01276263
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