ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Due to the lack of a convenient radiotracer of Li, we have used the stable isotopes, 6Li and 7Li, for unidirectional flux measurements in Lemna gibba L. (GI). Detection was performed using an ionic microanalyser. The conventional method of compartmental analysis has been improved in several ways. Growth of the plant samples has been taken into consideration. The possible non-uniqueness of the kinetic solution fitting the experimental points has been considered by numerical calculation. The derivatives and not only the direct functions have been used in order to detect whether a constant term had to be added to the first-order terms. The validity of the estimates of the characteristic parameters of transport has been checked by using efflux and influx data simultaneously. The “manipulation-shock” effects have been shown not to be negligible. The kinetic data have no simple biological significance per se, but they can serve to calculate cellular parameters of transport when combined with appropriate stereometric data. These considerations are relevant not only to our present problem, but to any type of compartmental analysis, whatever the plant sample and the absorbed substrate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1984.tb05903.x
