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  • 1
    ISSN: 1573-5087
    Keywords: alar ; apple ; daminozide ; ethylene ; fruit ; Malus domestica ; methionine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract At harvest, fruit from apple trees sprayed with daminozide (+daminozide) had lower levels of aminocyclopropane-1-carboxylic acid (ACC) and produced significantly lower amounts of ethylene than untreated (−daminozide) fruit. Flesh discs from the fruit of +daminozide and −daminozide trees were fed precursors of ethylene to determine how daminozide inhibits ethylene production. ACC was metabolized to ethylene regardless of treatment. Methionine (MET), however, was only converted to ethylene by −daminozide fruit, and only after the fruit had been maintained at 4 °C for 5 months. +Daminozide fruit failed to convert MET to ethylene at harvest, as well as after cold storage. When daminozide was added to the incubation media of flesh discs it did not inhibit ethylene production or the conversion of ACC to ethylene. The addition of daminozide did, however, inhibit the metabolism of exogenous MET to ethylene. Aminooxyacetate acid (AOA) blocked both the endogenous production of ethylene and that from MET feeds. Daminozide inhibits ethylene production by preventing the conversion of MET to ACC, but it does not appear to act as a simple competitive inhibitor of ACC synthase activity.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Endophyte ; Chewings fescue ; strong creeping red fescue ; ergovaline ; peramine ; lolitrem B ; chinch bug ; Epichloe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Four Chewings fescue and two strong creeping red fescue selections that had been artificially inoculated and stably maintained with four different endophytes were evaluated in feeding trials with chinch bugs (Blissus leucopterus hirtus). Significant differences in survival were found between the endophyte-inoculated plants and their endophyte-free counterparts. After seven days, 54.2% of chinch bugs were alive on endophyte-free tillers versus only 7.4% of chinch bugs fed tillers from endophyte-inoculated plants. Some differences were also found among the various plant–endophyte combinations. In Petri dish preference trials, chinch bugs showed a preference for the CA endophyte (obtained from a Chewings fescue) over the RC endophyte (obtained from a strong creeping red fescue) in Chewings fescue selection C1117. Only the inoculated plants produced erogvaline, peramine, and lolitrem B; moreover, significant differences were found among the plant–endophyte combinations in the levels of these alkaloids. The Chewings selections C1117 and C1090 produced more ergovaline, and C1090 more lolitrem B, than other plants, regardless of endophyte source. In the presence of the RC endophyte, more ergovaline and lolitrem B was produced than in the presence of the CA endophyte regardless of plant genotype. Both host plant and endophyte, therefore, contributed factors that determined alkaloid production. No significant correlations between chinch bug survival and alkaloid levels were found, however, and overall, no one plant genotype or endophyte source proved to be significantly more toxic than another to chinch bugs. Nevertheless, the results clearly demonstrated that artificial inoculations of endophyte-free fescue genotypes can produce plants with increased toxicity to chinch bugs.
    Type of Medium: Electronic Resource
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