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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 135 (1999), S. 709-720 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Studies of the link between planktonic and benthic stages of marine macroalgae have been hampered by the inability to identify planktonic stages from field samples. Here I describe a microphotometric method for identifying kelp zoospores from in situ plankton samples based on light-absorbance characteristics of zoospore plastids. Absorption spectra were obtained from plastids within Macrocystis pyrifera (L.) C. Agardh, Pterygophora californica Ruprecht, Eisenia arborea Areschoug and Laminaria farlowii Setchell zoospores released in the laboratory from adult specimens collected in the field; these are the dominant subtidal kelps in most southern California kelp forests. Absorption spectra were highly specific to each kelp species. Average absorbance decreased from P. californica to E. arborea to L. farlowii to M. pyrifera at most wavelengths between 400 and 750 nm. Discriminant-function analyses provided a significant discriminant rule, based on zoospore-plastid absorbance values at 25 different wavelengths, for identifying unknown zoospores among the four kelp species. Cross-validation techniques indicated that the discriminant rule could successfully identify 97.8% M. pyrifera, 96.4% P. californica, 86.2% E. arborea and 69.7% L. farlowii zoospores. Most misidentified E. arborea zoospores were identified as L. farlowii, and vice-versa; when the relatively rare E. arborea was pooled with L. farlowii and treated as a single complex, the discriminant rule could successfully identify 98.2% M. pyrifera, 97.4% P. californica and 89.5% E. arborea/L. farlowii zoospores. Coupled with a protocol for isolating kelp zoospores from non-kelp cells, this discriminant rule was used to determine kelp zoospore abundances and species-compositions of in situ plankton samples. This is the first description of a method for identifying macroalgal zoospores or spores from field samples.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2389
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Throughout the world there is a trend towards retaining crop residues rather than burning them. For this reason, changes in soil chemistry and aggregation in a Vertisol induced by 59 years of burning or green cane harvesting with or without annual fertilizer applications were investigated. Crop residues were either burnt prior to harvest with the harvest residues raked off (R1), burnt prior to harvest with the harvest residues left on the soil surface (R2), or left unburnt with all the trash left on the soil surface (R3). Concentrations of organic C in the surface 10 cm of soil increased with fertilizer applications and with increasing amounts of crop residue returned in the order R1 〈 R2 〈 R3. Fertilizer applications caused an accumulation of residual P in both inorganic (Pi) and organic (Po) forms. A sequential P fractionation showed that fertilizer P accumulated in both labile and recalcitrant Pi and Po forms, and trash retention caused an accumulation of recalcitrant Po. Concentrations of K decreased in the unfertilized R1 and R2 treatments because K reserves were depleted. By contrast, there was an increase in the concentrations of K in the fertilized R3 treatment. The soil became more acid on the fertilized and, to a lesser extent, trash retention plots. We attribute this to nitrification and subsequent nitrate leaching. Acidification resulted in a loss of exchangeable Ca and Mg, a decrease in ECEC, and an increase in the concentrations of total and monomeric Al in soil solution, in exchangeable Al3+ and in the buffering reserve of non-exchangeable Al associated with organic matter. Aggregate stability was increased by increasing crop residues but decreased by fertilizer applications. The decrease was attributed to an increase in the proportion of exchangeable cations present in monovalent form due to applications of fertilizer K and leaching of Ca and Mg. We conclude that trash retention and annual fertilizer applications have substantial long-term effects on both organic matter status and soil pH and therefore on other soil chemical and physical properties.
    Type of Medium: Electronic Resource
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