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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 42 (1986), S. 96-97 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 15 (1996), S. 398-402 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A highly sensitive and specific method, termed PCR/NheI, for the detection of genes coding for SHV extended-spectrum β-lactamases (ESBL) in clinical isolates is presented. It is based on polymerase chain reaction (PCR) amplification of thebla SHV genes, followed by restriction withNheI. Due to the glycine (position 238) (SHV-non-ESBL) → serine (position 238) (SHV-ESBL) mutation, only PCR fragments from the genes coding for SHV-ESBLs were cleaved. A commercially available test for ESBLs, the E test ESBL, identified 52% of our 29 clinical isolates carryingbla SHV-ESBL genes as ESBL producers.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 12 (1993), S. 928-937 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract One susceptible and two multiply resistant isolates ofListeria monocytogenes from a patient suffering from prosthetic valve endocarditis are described. They could not be distinguished by several typing methods. Two isolates were resistant to chloramphenicol, macrolide/lincosamide/streptogramin antibiotics and tetracycline. The resistance determinants were located on a 39 kb plasmid pWDB100 that was transferable by filter mating to several gram-positive bacteria. Evidence was obtained to support the hypothesis that the resistant variant had primarily infected the patient's blood and prosthetic valve, and later lost the resistance plasmid. The three resistance determinants showed homology to other known markers,cat221/cat223,ermB andtetM, which are frequently found in different gram-positive genera. Plasmid pWDB100 showed extensive homology to theStreptococcus agalactiae broad-host-range plasmid pIP501. It was also very similar to two listerial plasmids found in France. Thus, plasmid pWDB100 and the homologous plasmids from France, although isolated in geographically distant regions, may illustrate spread of a plasmid and its relatives.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Für die mikrobiologische Untersuchung von Peritoneal-Flüssigkeit bei CAPD-Patienten wird oft Konzentration der Bakterien empfohlen. Diese Studie befaßt sich mit der mikrobiellen Ausbeute bei Zentrifugation von verschiedenen Volumina. Proben von 50 bzw. 1000 ml derselben Dialysatsäcke wurden zentrifugiert und mittels Festmedien und Anreicherungs-Bouillon auf aerobe und anaerobe Mikroorganismen untersucht. Mit einer Ausnahme zeigten alle 28 50-ml-Proben (96%) das gleiche Kulturresultat wie die entsprechenden 1000-ml-Proben, was auf die Nutzlosigkeit einer Zentrifugation von mehr als 50 ml hinweist. Zudem scheint es besser, zwei oder mehr aufeinanderfolgende Säcke pro Peritonitis-Episode zu untersuchen, denn in sieben von neun (77%) solchen Episoden wurden Mikroorganismen gefunden, während das nur in fünf (62%) von acht Episoden mit nur einem untersuchten Sack der Fall war. Zusätzlich wurden signifikant mehr positive Kulturen registriert (14 von 17 verglichen mit 5 von 11; p〈0.05), wenn die Proben vor Antibiotika-Applikation abgenommen wurden. Der entfernte Katheter eines Patienten war kolonisiert mit Bakterien, die vermutlich in Form eines „Biofilms“ vorlagen. Diese Tatsache scheint von besonderer Bedeutung für CAPD-Patienten zu sein und verdient, Gegenstand weiterer Forschung zu sein.
    Notes: Summary Concentration of peritoneal fluid of continuous ambulatory peritoneal dialysis (CAPD) patients has often been recommended for microbiological examination. This study presents data about the yield of microorganisms of different volumes through centrifugation. Twenty-eight samples of 50 ml and 1000 ml of the same dialysate bags were centrifuged, and assays were made for aerobic and anaerobic organisms on solid media and in enrichment broth. All pairs of 50 ml and 1000 ml samples except one (96%) showed identical results, suggesting that it is unnecessary to centrifuge more than 50 ml of fluid. Furthermore, it seems better to examine two or more consecutive bags per peritonitis episode, since microorganisms were found in seven of nine (77%) such episodes, compared to only five of eight (62%) episodes with only one bag. In addition, significantly more positive cultures were recorded (14 of 17 vs. 5 of 11; p〈0.05) if samples were taken before administration of antibiotics. One patient's catheter was colonized with bacteria probably arranged in a biofilm. This fact seems to be of particular importance for CAPD patients and should be studied further.
    Type of Medium: Electronic Resource
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