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  • 1
    Electronic Resource
    Electronic Resource
    Effects of sudden temperature shifts on pure cultures of four strains of freshwater bacteria (1979)
    Springer
    Microbial ecology 5 (1979), S. 17-26 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three psychrotrophic and one mesophilic strains were isolated from winter water samples of different freshwater biotopes and identified asCytophaga johnsonae (C-21),Cytophaga sp. (M-17),Pseudomonas fluorescens (KD), andEnterobacter cloacae (BS-2). Temperature shift-up experiments with emphasis on low temperatures were carried out with aerated pure batch cultures in glucose mineral medium. The effects of sudden temperature increases on growth rates and substrate conversion were investigated. All three psychrotrophic strains in the temperature increase experiments at low temperatures showed differing reactions within the linear zone of the Arrhenius plot. TheC. johnsonae (C-21) shift-up cultures adjusted the growth rate immediately to the rate of the temperature adapted cultures, whereasCytophaga sp. (M-17) shift-up cultures showed a lower andP. fluorescens (KD) a higher growth rate. The mesophilicE. cloacae (BS-2), likeC. johnsonae (C-21), adjusted immediately to the new growth rate. Substrate conversion increased in all experiments immediately after the shift-up. The extracellular substrate conversion byP. fluorescens (KD) of glucose to gluconate and 2-ketogluconate was particularly affected by the sudden temperature increase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010574
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transfer RNAs as genotypic fingerprints of eubacteria (1990)
    Springer
    Archives of microbiology 153 (1990), S. 299-304 
    Details
    ISSN: 1432-072X
    Keywords: tRNA ; 5S rRNA ; RNA profiles ; Chemotaxonomy ; Genotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new method was developed for rapid genotypic identification and classification of bacteria. The method is based on high resolution gel electrophoresis of the stable, low molecular weight (LMW) RNA fraction of single bacterial strains. This fraction comprises the total transfer RNA pool and the 5S ribosomal RNA. On a one-dimensional gel, every eubacterial strain exhibited a distinct LMW RNA profile, a set of bands belonging to three different size classes: 5S rRNAs (110–131 nt), class 2 tRNAs (82–96 nt) and class 1 tRNAs (72–79 nt). LMW RNA profiles of members of five of the ten major eubacterial groups, previously defined by 16S rRNA sequence analysis, were highly diverse. For some major groups, like flavobacteria and planctomyces, the distinctive sizes of their 5S rRNAs allowed the assignment of strains to these groups. More specific taxonomic information was gained from analysis of the tRNA part of the profile. Strains could be grouped as species and genera due to species- and genus-specific tRNA bands. From an evolutionary point of view, this order found in the total tRNA pool of eubacteria could indicate that cytoplasmic tRNA evolution reflects ribosomal RNA evolution. Given the universality of tRNAs, it is to be expected that their electrophoretic mobility profiles may serve as a convenient RNA fingerprint for defining bacterial species operationally and for identifying new genotypes by differing patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249086
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  • 3
    Electronic Resource
    Electronic Resource
    Glucose uptake of Cytophaga johnsonae studied in batch and chemostat culture (1982)
    Springer
    Archives of microbiology 133 (1982), S. 289-294 
    Details
    ISSN: 1432-072X
    Keywords: Cytophaga johnsonae ; Glucose uptake ; Substrate specificity ; Substrate affinity ; Chemostat operation ; Facultative oligotrophy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of different physiological states on the glucose uptake and mineralization by Cytophaga johnsonae, a freshwater isolate, was examined in batch and chemostat cultures. At different growth rates under glucose limitation in chemostat cultures, different uptake patterns for 14C labeled glucose were observed. In batch culture and at high growth rates the glucose uptake potential showed a higher maximum velocity and a much lower substrate affinity than at lower growth rates. These findings and the results of short-term labeling patterns could be explained by two different glucose uptake mechanisms which enable the strain to grow efficiently both at high and low substrate concentrations. Substrate specificity studies showed that a structural change of the C-2 atom of the glucose molecule was tolerated by both systems. The consequences of these results for the ecophysiological classification of the Cytophaga group and for the operation of continuous cultures are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00521292
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  • 4
    Electronic Resource
    Electronic Resource
    Grazing of protozoa and its effect on populations of aquatic bacteria (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 35 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Predation by bacterivorous protists in aquatic habitats can influence the morphological structure, taxonomic composition and physiological status of bacterial communities. The protistan grazing can result in bacterial responses at the community and the species level. At the community level, grazing-induced morphological shifts have been observed, which were directed towards either larger or smaller bacterial sizes or in both directions. Morphological changes have been accompanied by changes in taxonomic community structure and bacterial activity. Responses at the species level vary from species to species. Some taxa have shown a pronounced morphological plasticity and demonstrated complete or partial shifts in size distribution to larger growth forms (filaments, microcolonies). However, other taxa with weak plasticity have shown no ability to reduce grazing mortality through changes in size. The impact of protistan grazing on bacterial communities is based on the complex interplay of several parameters. These include grazing selectivity (by size and other features), differences in sensitivity of bacterial species to grazing, differences in responses of single bacterial populations to grazing (size and physiology), as well as the direct and indirect influence of grazing on bacterial growth conditions (substrate supply) and bacterial competition (elimination of competitors).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00794.x
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  • 5
    Electronic Resource
    Electronic Resource
    Rotifer population dynamics in response to increased bacterial biomass and nutrients: a mesocosm experiment (1993)
    Springer
    Hydrobiologia 255-256 (1993), S. 171-175 
    Details
    ISSN: 1573-5117
    Keywords: mesocosm ; rotifers ; bacteria ; nutrients ; population response ; food chain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The impact of organic nutrients and massive addition of bacteria was followed in lake water mesocosms in a eutrophic lake. Increased DOM initiated a sequence of trophic responses indicated by rapid increases in bacterioplankton, protozoa, and algal biomass. The populations of Keratella cochlearis and Keratella quadrata showed a distinct response by rapid increase in birth rate followed by maxima of production and abundance. This succession clearly reflected the trophic position of these rotifer populations in the food chain. A reverse response was observed in Conochilus unicornis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025836
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    Paper (German National Licenses)
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