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  • 1
    Electronic Resource
    Electronic Resource
    Expression of genes from the marine bacteriumAlteromonas haloplanktis 214 inEscherichia coli K-12 (1985)
    Springer
    Archives of microbiology 142 (1985), S. 248-252 
    Details
    ISSN: 1432-072X
    Keywords: Alteromonas haloplanktis ; Marine bacteria ; Escherichia coli ; Gene bank ; Gene expression ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA from the marine bacteriumAlteromonas haloplanktis 214 was partially digested withSau 3A and inserted into theBam HI site of the cloning vector pBR322. The ligation mixture was used to transformEscherichia coli HB101. The gene bank plasmid preparation obtained was used to transformEscherichia coli K-12 strain EO2717, an organism auxotrophic for histidine, arginine, adenine, uracil and thiamin. Prototrophic transformants for each of the five metabolites were isolated using appropriate minimal media for selection. Plasmids isolated from each of the transformants were shown by hybridization to containA. haloplanktis DNA and to be capable of complementing the appropriate mutation inE. coli EO2717. Restriction maps showed that each of the plasmids was different.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00693398
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of inverted IS-length sequences in the E. coli K12 genome (1976)
    [s.l.] : Nature Publishing Group
    Nature 264 (1976), S. 191-193 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Formation of intrastrand DNA hybrid structures. The upper portion of the figure depicts a slightly nicked DNA duplex. The letters each denote a block of nucleotides; the sequences complementary to each block are indicated by the corresponding primed letter. The sequence r1 r2 r3 is repeated ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/264191a0
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    DNA sequences homologous to the T DNA region of Agrobacterium tumefaciens are present in diverse Rhizobium species (1982)
    Springer
    Molecular genetics and genomics 188 (1982), S. 361-369 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary DNA sequences homologous to the T DNA region of the octopine-type Ti plasmid from Agrobacterium tumefaciens are present in different Rhizobium species. Plasmid DNA from each of two R. leguminosarum, two R. meliloti, and four slow-growing Rhizobium strains examined contain restriction endonuclease fragments that hybridize with the T DNA region, or with DNA sequences at or near the adjacent Ti plasmid transfer (ra) region. Four different BamHI fragments that contain homology to the T DNA region were cloned from R. leguminosarum 300 plasmid DNA. Cloned fragments of 5.9 kb and 10.3 kb hybridize to each other and are homologous to sequences which map at the right boundary region (EcoRI fragment 24) of the “core” T DNA. Ti plasmid sequences homologous to those present in cloned fragments of 10.9 kb and 2.0 kb map in adjacent fragments near the tra genes, approximately 10 kb to the right of the “core” T DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330035
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  • 4
    Electronic Resource
    Electronic Resource
    A plasmid sequence from Rhizobium leguminosarum 300 contains homology to sequences near the octopine TL-DNA right border (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 580-584 
    Details
    ISSN: 1617-4623
    Keywords: Rhizobium ; Agrobacterium ; TDNA ; Insertion sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The DNA sequence from a Rhizobium leguminosarum 300 (RL300) plasmid that contains homology to the Tc-DNA of Agrobacterium tumefaciens is described. The RL300 sequence has 78% homology to a 359 bp sequence in the Tc-DNA of pTi15955. The RL300 homology starts approximately 100 bp from the 24 bp border sequence of the TL-DNA and ends approximately 3 bp from an IS66 homolog in the Tc-DNA. An unusual feature of the RL300 homology is the presence of 81 bp direct repeats with Tc-DNA homology, separated by 201 bp. One end of each direct repeat has a 12 bp palindrome. Four cloned sequences of RL300 with homology to the T DNA region were hybridized to plasmid lysates of RL300 derivatives to determine the source of each plasmid. The sequenced homolog, originally on pRH228, was isolated from pRL7JI; the other 3 homologs were isolated from the transmissable plasmids pRL7JI (pRH235) and pRL8JI (pRH235 and pRH236).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331166
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    Paper (German National Licenses)
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