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  • 1
    ISSN: 1432-2285
    Keywords: Altitude ; Carbon isotope ratio ; Clusiaceae ; Crassulacean acid metabolism ; Deuterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Samples of the Clusiaceae generaClusia, Oedematopus andDystovomita were collected at various sites and different altitudes in northern and south-western Venezuela. Analyses of stable isotopes of carbon and hydrogen and of leaf-nitrogen levels were performed on the dried samples. Correlations among these variables, i.e. carbon isotope discrimination (Δ), hydrogen isotope ratio (δD) and N-levels, and with altitude were assessed. In the samples, where values of Δ above 15‰ indicate predominant performance of C3 photosynthesis, there were slight tendencies of increasing Δ, δD and N-levels with increasing altitude and of increasing Δ with increasing N. Although these correlations taken separately were not statistically significant, they support each other and indicate increasing transpiration and increased leaf-nutrient supply at increasing altitude. Performance of crassulacean acid metabolism (CAM) in species ofClusia appears to be restricted to altitudes below 1500 m a.s.l. There was a significant negative correlation of Δ with altitude in the samples, where values of Δ below 10‰ indicated predominant performance of CAM. This suggests that phases II and IV of CAM are progressively suppressed towards the upper altitudinal limit of CAM inClusia in northern Venezuela. It is concluded that among the large number of environmental factors and combinations thereof, which determine the expression of CAM inClusia and trigger C3-CAM transitions in C3/CAM intermediate species, low availability of water is the most important.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0931-1890
    Keywords: Key words Chlorophyll a fluorescence quenching ; Citric acid ; Malic acid ; Photochemistry ; Photosynthetic electron transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Sandy plains are characteristic of the coastal region of Brazil. We investigated the diel patterns of changes in organic acid levels, leaf conductance and chlorophyll a fluorescence for sun-exposed and shaded plants of Clusia hilariana, one of the dominant woody species in the sandy coastal plains of northern Rio de Janeiro state. Both exposed and shaded plants showed a typical CAM pattern with considerable diel oscillations in organic acid levels. The degradation of both malic and citric acids during the midday stomatal closure period could lead to potential CO2 fixation rates of 28 μmol m – 2 s – 1 in exposed leaves. Moreover, exposed leaves exhibited large increases in total non-photochemical quenching (qN) accompanied by a substantial decrease in effective quantum yield during the course of the day. However, these potential high rates of CO2 fixation and the increases in qN of exposed plants were not enough to maintain the primary electron acceptor of photosystem II (QA) in a low reduction state, similar to that of shaded plants. As a result, there was a moderate increase in the reduction state of QA throughout the day. Most of the decline in photochemical efficiency of exposed leaves of C. hilariana was reversible, as evidenced by the high levels of pre-dawn potential quantum yields (Fv/Fm) and their rapid recovery after sunset. However, the depletion of the organic acid pool in the afternoon resulted in an accentuated subsequent drop in Fv/Fm, suggesting that prolonged periods of water stress accompanied by high irradiance levels may expose plants of C. hilariana in unprotected habitats to the danger of photoinhibition.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0931-1890
    Keywords: Key words Chlorophyll fluorescence ; Stomatal ; conductance ; Rock outcrops ; Sand dune formations ; Photosynthesis diurnal changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Diurnal measurements of chlorophyll fluorescence parameters and stomatal conductance to water vapour were carried out in five woody species and a bromeliad from two coastal vegetation types in Brazil, the rock outcrop of Pao de Acúcar and the sand dunes of Macaé. The environmental conditions of both study sites are characterized by high temperatures and light levels. The studied species comprised C3 plants with different degrees of stomatal closure during the day, overall daily stomatal conductance and a plant with a typical CAM pattern. Plants on Pao de Acúcar exhibited only a small decline in potential quantum yield throughout the day. The non-photochemical quenching and the approximate photosynthetic electron transport rates were maximal during the peak of irradiance. In Macaé, light response curves of fluorescence parameters in the CAM-tree Clusia hilariana showed a clear differentiation between phases III and IV of CAM. In phase III, decarboxylation of organic acids probably maintained high internal CO2 levels and there was only a small decrease in photochemical quenching with saturating light levels. In phase IV, the depletion of the organic acid pool and low stomatal conductances resulted in much lower levels of effective quantum yield and a substantial increase in both 1-qP and non-photochemical quenching with increasing light levels. This behaviour during phase IV in the CAM-tree C. hilariana was comparable to the performance of the C3-Clusia C. aff parviflora at Pao de Acúcar. It is seen that both the C3 species and the CAM tree occurring in the two coastal communities effectively adjust their photochemical efficiency to environmental conditions and especially to diurnal variations of stress.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0931-1890
    Keywords: Key words Altitude ; Carbon isotope ratio ; Clusiaceae ; Crassulacean acid metabolism ; Deuterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Samples of the Clusiaceae genera Clusia, Oedematopus and Dystovomita were collected at various sites and different altitudes in northern and south-western Venezuela. Analyses of stable isotopes of carbon and hydrogen and of leaf-nitrogen levels were performed on the dried samples. Correlations among these variables, i. e. carbon isotope discrimination (Δ), hydrogen isotope ratio (δD) and N-levels, and with altitude were assessed. In the samples, where values of Δ above 15 ‰ indicate predominant performance of C3 photosynthesis, there were slight tendencies of increasing Δ, δD and N-levels with increasing altitude and of increasing Δ with increasing N. Although these correlations taken separately were not statistically significant, they support each other and indicate increasing transpiration and increased leaf-nutrient supply at increasing altitude. Performance of crassulacean acid metabolism (CAM) in species of Clusia appears to be restricted to altitudes below 1500 m a. s. l. There was a significant negative correlation of Δ with altitude in the samples, where values of Δ below 10 ‰ indicated predominant performance of CAM. This suggests that phases II and IV of CAM are progressively suppressed towards the upper altitudinal limit of CAM in Clusia in northern Venezuela. It is concluded that among the large number of environmental factors and combinations thereof, which determine the expression of CAM in Clusia and trigger C3-CAM transitions in C3/CAM intermediate species, low availability of water is the most important.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0931-1890
    Keywords: Key words Chlorophyll a fluorescence ; Clusia ; Crassulacean acid metabolism (CAM) ; Electron transport rate through photosystem II ; Malate decarboxylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Changes in chlorophyll a fluorescence during the day and diurnal-changes of net CO2-exchange and organic acid contents were determined in two species of the genus Clusia during the dry season in Venezuela. The investigations included plants of the C3/CAM intermediate species Clusia minor and the C3 species C. multiflora growing at exposed and shaded sites. Both species showed a C3 pattern of net CO2-exchange at the exposed site. In the shade under extreme drought stress C. minor showed a weak expression of CAM without CO2-uptake during the afternoon (phase IV of CAM). C. multiflora growing in the shade exhibited a C3-pattern of net CO2-exchange and a small but significant nocturnal accumulation of citrate. Shaded plants of C. minor were able to double their light utilisation for electron transport and to reduce non-photochemical quenching during phase III compared to phase II of CAM. Furthermore, increase of electron transport rate through photosystem II in phase III of CAM is correlated to decarboxylation of malate. At the exposed site C. multiflora was less negatively affected by high PPFD than C. minor. This was shown by a lower reduction of potential electron quantum yield (Fv/Fm) and higher light utilisation of electron transport of C. multiflora compared to C. minor. At the exposed site C. minor did not make use of the CAM option to increase light utilisation of electron transport and to reduce non-photochemical quenching as did the plants growing in the shade.
    Type of Medium: Electronic Resource
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