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  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Phytopathology 32 (1994), S. 363-386 
    ISSN: 0066-4286
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 23 (1972), S. 389-418 
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 45 (1996), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Fifty-one cowpea (Vigna unguiculata) cultivars and lines were tested by mechanical inoculation against seven geographically and pathogenically diverse isolates of blackeye cowpea mosaic potyvirus (BICMV), to identify genetic resources with comprehensive BICMV resistance. Five genotypes, IT 80S 2049, Big Boy, Corona, Serido, and Tennessee Cream #8 were immune from all seven isolates, and an additional three genotypes, TVu-2657, TVu-2740, and TVu-3433, were immune from all isolates except PU-7B, an aberrant B1CMV isolate. The diversity among BICMV isolates was illustrated by the range of responses to inoculation among cowpea genotypes, many of which were either immune from or tolerant of individual B1CMV isolates.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 45 (1996), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Seedlings from 182 cowpea (Vigna unguiculata) pre-introductions/germplasm accessions from 12 countries were tested under greenhouse conditions for six seed-borne viruses. Twenty-one (13.3%) accessions from eight countries were found to be seed-infected with one of the three following viruses: blackeye cowpea mosaic (BlCMV) and cowpea aphid-borne mosaic (CABMV) potyviruses, and cucumber mosaic cucumovirus (CMV). Natural seed transmission incidence of 0–6.9%, 0–13.3%, and 0–2.0% were determined for BlCMV, CABMV and CMV respectively.  Another set of 2930 cowpea germplasm accessions, mostly from Botswana and Senegal (Africa), were examined under field conditions for detection and identification of seed-borne viruses. Only CABMV was detected in this material. Most of the lines were free from other viruses reported in cowpea seed. Eight isolates of BlCMV and 28 isolates of CABMV were derived from cowpea pre-introductions/germplasm accessions evaluated under greenhouse and field conditions.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Eleven isolates of pea seedborne mosaic virus (PSbMV) from U.S. seedlots were compared by thin-section electron microscopy to appraise the cytopathological diversity among isolates. Nine isolates uniformly induced formation of typical pinwheel inclusions, two of these also inducing tonoplast aggregates (TA) in a few cells. One isolate produced principally TA, accompanied in a few cells by pinwheel inclusions. The remaining isolate induced formation of dense bodies and laminated aggregates like those reported for bean yellow mosaic virus (Kamei et al., 1969). Other cytological abnormalities induced by one or more PSbMV isolates included extensive masses of convoluted endoplasmic reticulum, aggregates of viruslike particles, and crystalline inclusions consisting of ultrastructural hexagons. Symptoms induced by the eleven isolates in seedlings from which they originated fell within the range previously reported for this virus (Hampton, 1972). Root parenchyma cells from PSbMV-infected plants contained few inclusions, but instead contained extensive masses of viruslike particles that occupied most of the cytoplasmic volume in some cells. Samples of purified virus prepared separately from infected root and leaf tissues were morphologically indistinguishable.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 141 (1996), S. 1961-1977 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Partial nucleotide sequences of selected isolates of bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) were determined. Based on these sequences and previously published sequence data, a reverse transcription, polymerase chain reaction (RT-PCR) in combination with restriction endonuclease analyses, was developed for molecular detection of BCMV, BCMNV and some viral pathogroups (PG). Specific detection of the two viruses was accomplished by constructing two virus-specific primer pairs that amplified a PCR product specific for each virus. By application of RT-PCR, four BCMV-PG-V isolates were differentiated from isolates of BCMV pathogroups I, II, IV and VII. Distinction of two BCMNV pathogroups (PG-III and PG-VI) was achieved by restriction enzyme XbaI digestion of BCMNV PCR products. However, no combination of tested restriction enzymes distinguished all five recognized BCMV pathogroups. A primer pair Dts/Uny 15 proved to be specific for BCMV pathogroup PG-V. Thus, by a combination of RT-PCR and restriction enzyme analyses, it was possible to differentiate both viruses, two pathogroups of BCMNV, and one pathogroup of BCMV from the others.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plant pathology 102 (1996), S. 411-419 
    ISSN: 1573-8469
    Keywords: CABMV ; isolates ; genotypes ; immune ; resistant ; tolerant ; hypersensitive ; pathogenic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fifty-one cowpea (Vigna unguiculata (L.) Walp.) genotypes were tested by mechanical inoculation with seven geographically diverse isolates of cowpea aphid-borne mosaic potyvirus to identify resistant sources. Of 51 genotypes three, TVu-401, TVu-1582 and TVu-1593 were found immune to all the seven isolates. Forty-five genotypes gave different reactions to individual isolates. Several immune, resistant and tolerant genotypes against each isolate were identified. A considerable evidence of pathogenic variability among the virus isolates was also observed.
    Type of Medium: Electronic Resource
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