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  • 1
    Electronic Resource
    Electronic Resource
    Methyl jasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures (1996)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 14 (1996), S. 1129-1132 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Taxus cell culture may be an alternative source of paclitaxel and related taxane production. Significantly increased amounts of paclitaxel and baccatin III were observed in cultured cells of Taxus species after exposure to methyl jasmonate. Among the three species of Taxus tested, Taxus media ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0996-1129
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  • 2
    Electronic Resource
    Electronic Resource
    Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon (1981)
    Springer
    Plant cell reports 1 (1981), S. 59-60 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shikonin derivatives were produced from cell suspension cultures of Lithospermum erythrorhizon for the first time. The results of studies on various culture media showed that the type of nitrogen source in the medium greatly influenced the production of these derivatives. When nitrate was the nitrogen source, stable production of shikonin derivatives by the cell suspension culture took place. We also found that ammonium as the nitrogen source conspicuously inhibited the production of shikonin derivatives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269272
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  • 3
    Electronic Resource
    Electronic Resource
    Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon (1981)
    Springer
    Plant cell reports 1 (1981), S. 61-63 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effects. With the new, M-9, medium produced from these studies the yield of shikonin derivatives was 1400 mg/l and the yield for dried cells was about 12%, whereas it was 120 mg/l, or about 2% with White's medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269273
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A new bioassay system for screening high berberine-producing cell colonies of Thalictrum minus (1987)
    Springer
    Plant cell reports 6 (1987), S. 194-196 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and the concentration of berberine which has been released from the cells into the agar piece is assayed by the antibacterial activity against Bacillus cereus MT2026. Screening of 1000 cell colonies by the “agar piece method” has resulted in the isolation of four, high berberine-producing cell lines, although they have been found to be more or less unstable with respect to the biosynthetic capability during successive subcultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268477
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  • 5
    Electronic Resource
    Electronic Resource
    Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon (1987)
    Springer
    Plant cell reports 6 (1987), S. 8-11 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differences in the production of shikonin derivatives by callus and suspension cultures of Lithospermum erythrorhizon Sieb. et Zucc. were examined. When Linsmaier and Skoog medium was used in suspension cultures, cell growth was not accompanied by the production of shikonin compounds. Shikonin derivatives were produced, however, when this medium was used in callus cultures. Differences in shikonin production were examined in terms of the nutrient supply, the effect of the agar itself, and the oxygen supply. Shikonin derivatives could be produced without agar by keeping the cells exposed to air while providing an adequate supply of nutrients. In callus cultures, the production of shikonin compounds was reduced remarkedly when the oxygen concentration in the atmosphere was lowered, evidence that shikonin production during L. erythrorhizon cell growth on Linsmaier and Skoog agar medium is enhanced by an abundant supply of oxygen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269727
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  • 6
    Electronic Resource
    Electronic Resource
    A study of malabsorption in pancreatic cancer (1996)
    Springer
    Journal of gastroenterology 31 (1996), S. 81-85 
    Details
    ISSN: 1435-5922
    Keywords: pancreatic cancer ; malabsorption ; d-xylose absorption test ; pancreatic function diagnostant test ; carotene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The type, incidence, and severity of malabsorption in patients with pancreatic cancer were investigated. The following absorption tests were performed; pancreatic function diagnostant (PFD) test, measurement of serum carotene levels, and 5 gd-xylose absorption test. Rates of abnormality in the tests were 75.7% of 37, 54.2% of 48, and 54% of 50 patients with pancreatic cancer, respectively. In particular, a marked decrease of values in the xylose absorption test was characteristic and more often recognized in patients with carcinoma of the pancreas with occlusion of the superior mesenteric vein. The presence of malabsorption (disturbed transport through the portal vein), in addition to maldigestion of nutrients, is suggested to induce severe malnutrition in patients with pancreatic cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01211191
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