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  • 1
    Electronic Resource
    Electronic Resource
    Inhibition of cancer cell motility and invasion by interleukin-12 (1995)
    Springer
    Clinical & experimental metastasis 13 (1995), S. 396-404 
    Details
    ISSN: 1573-7276
    Keywords: cell motility ; colon cancer ; interleukin-12 ; invasion ; metastasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Tumour cell motility and attachment are crucial requirements in the formation of metastatic lesions. These properties are affected by a number of cytokines including hepatocyte growth factor/scatter factor (HGF/SF) and several immunoregulatory proteins, including interleukin-12 (IL-12). Although IL-12 has been reported to exhibit potent anti-tumour effects in vivo, a direct effect of IL-12 on cancer cells has not been reported. We show here that IL-12 directly inhibited the attachment of the human colon cancer cell lines HRT18, HT29 and HT115 to Matrigel, HGF/SF-stimulated cell motility and HGF/SF-induced cell invasion through a reconstituted basement membrane. IL-12 did not affect the growth of these cell lines. Flow cytometry, Western analysis and immunohistochemistry revealed an up-regulation of E-cadherin cell-surface adhesion molecules. These direct effects of IL-12 on colon cancer cells suggest a potentially important role for IL-12 in metastasis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00121916
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Regulation of desmosomal cell adhesion in human tumour cells by polyunsaturated fatty acids (1997)
    Springer
    Clinical & experimental metastasis 15 (1997), S. 593-602 
    Details
    ISSN: 1573-7276
    Keywords: cell adhesion ; desmoglein ; E-cadherin ; gamma linolenic acid ; invasion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Desmosomes are key structures in cell-cell adhesion. In this study we examined the effect of n-6 essential fatty acids on the expression of desmoglein (Dsg), desmosomal cadherin and the formation of desmosomes in E-cadherin negative human breast, colon and lung cancer cells and melanoma cells. Electron microscopy revealed that cells cultured with gamma linolenic acid (GLA) showed increased cell-cell adhesion together with an increase in the formation of desmoglein-containing desmosomes. Western blotting studies of cellular proteins demonstrated that, following culture with fatty acids, Dsg expression was modified, with the greatest increase seen after GLA treatment. Other fatty acids increased Dsg expression, but to a lesser extent. It is concluded that GLA regulates desmosome-mediated cell-cell adhesion in human cancer cells, particularly in cells without E-cadherin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018435229087
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Inhibition of HGF/SF-induced breast cancer cell motility and invasion by the HGF/SF variant, NK4 (2000)
    Springer
    Breast cancer research and treatment 59 (2000), S. 245-254 
    Details
    ISSN: 1573-7217
    Keywords: breast cancer ; hepatocyte growth factor/scatter factor ; metastasis ; NK4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract NK4 is a variant form of HGF/SF, comprising the N-terminal and subsequent four kringle domains of mature HGF/SF. HGF/SF is a multifunctional cytokine that enhances the metastatic behaviour of tumour cells in vitro by stimulation of the c-met receptor tyrosine kinase and has been implicated in the development of tumour metastasis in vivo. The aims of this study were to further investigate the potential antagonistic effects of the recently described variant form of HGF/SF, NK4, on HGF/SF activity in breast cancer cells. All cell lines used expressed both the HGF/SF receptor gene and protein as shown by RT-PCR and Western blotting. NK4 inhibited HGF/SF-induced tumour cell invasion through an artificial basement membrane. Tumour cell motility and scattering induced by HGF/SF were also dramatically reduced by the inclusion of NK4. Immunoprecipitation studies revealed that NK4 inhibited the phosphorylation of the c-met receptor in response to HGF/SF. Treatment of these cells with NK4 alone did not have any significant effects on their metastatic behaviour. From this data we conclude that NK4 demonstrates significant antagonistic properties towards HGF/SF, inhibiting HGF/SF-stimulated breast tumour cell invasion, motility and migration. NK4 may therefore be of potential benefit in the development of anti-metastasis therapies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006348317841
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    Paper (German National Licenses)
    Fulltext
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