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  • 1
    Electronic Resource
    Electronic Resource
    Ethylene stimulates the accumulation of ripening-related mRNAs in tomatoes (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 10 (1987), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Seven cDNA clones for ripening-related mRNAs were used as hybridization probes to study the regulation of gene expression during tomato (Lycopersicon esculentum Mill) ripening. The mRNAs corresponding to each clone were detected by Northern blotting and the sizes of the transcripts compared with the molecular weights of polypeptides identified previously by hybrid-release translation. Dot hybridization to poly(A)+ mRNA from leaves, roots, unripe and ripe tomatoes established that five of the clones encoded mRNAs that were ripening specific and two encoded mRNAs also expressed in other organs. Appearance of these ripening-specific mRNAs was correlated with ethylene production as ripening commenced and occurred before lycopene synthesis began. Thereafter, the mRNAs increased to maximum levels at the orange stage and then declined slowly as fruit turned red. When mature green fruit were picked before any increase in ethylene synthesis occurred and placed in air plus 10 cm3 m−3 ethylene, substantial changes in translatable mRNA were noted within 30 h. Five mRNAs that increased in amount directed the synthesis in vitro of polypeptides similar in size to those encoded by the five ripening-specific clones. Several different patterns of mRNA accumulation were observed, with increases in concentration of the order of 300-fold.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1365-3040.ep11602134
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic map locations for orthologous Vp1 genes in wheat and rice (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 281-284 
    Details
    ISSN: 1432-2242
    Keywords: Key words Maize ; Vivipary ; Grain colour ; Dormancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Chromosome locations for gene orthologues of the dormancy-related maize transcription factor VIVIPAROUS-1, encoded by the Vp1 locus on maize chromosome 3, were determined in wheat (Triticum aestivum L.) and rice (Oryza sativa L.) via linkage to markers on existing molecular maps using a cDNA of a wheat Vp1 orthologue as a probe in genomic Southern analyses. Vp1-orthologous loci were detected on the long arms of wheat chromosomes 3A, 3B and 3D [Xlars10 (taVp1) loci] and rice chromosome 1 (osVp1), in line with previous evidence of synteny between these regions of the rice and wheat genomes and chromosome 3 of maize. The wheat loci mapped some 30 cM from the centromeres and some 30 cM proximal to the red grain (R) loci that control seed colour and coat-imposed dormancy. This unequivocal, genetic separation of the Vp1 and R loci may offer an opportunity for improving resistance to pre-harvest sprouting in wheat by combining the coat-imposed dormancy associated with red seed colour and true embryo dormancy regulated by Vp1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051069
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    Paper (German National Licenses)
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