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  • 1
    Electronic Resource
    Electronic Resource
    Gibberellin status and responsiveness in shoots of tall and dwarf genotypes of diploid rye (Secale cereale) (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 89 (1993), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The recessive dwarfing alleles of rye (Secale cereale L.), ct1 and ct2, caused a 35–55% reduction in the length of leaf 2 compared with corresponding tall lines grown at both 10°C and 20°C. The dwarf lines were 45–50% as responsive to applied GA3 as the tall lines at 20°C but the absolute GA-responsiveness of the dwarfs was greater at 10°C than at 20°C. There was no significant difference in the contents of GA19, GA20, GA29, GA1, GA3 and GA8 in the leaf extension zone of tall and dwarf seedlings grown at 20°C. It was concluded that the mechanism whereby GA homeostasis is maintained is functional in both tall and dwarf lines despite marked differences in leaf extension rate. The recessive rye mutations may cause loss of function late in the GA-cell elongation pathway or, alternatively, indirectly affect GA-responsiveness in vegetative tissues. The genetic and physiological evidence indicates that ct1 and ct2 are unrelated to the GA-insensitive Rht genes in hexaploid bread wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1993.tb00159.x
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  • 2
    Electronic Resource
    Electronic Resource
    Hormonal regulation of α-amylase gene expression in germinating wheat (Triticum aestivum) grains (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 100 (1997), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The diffusion of GA1and GA3from the embryo, and the decline in ABA content of endosperm, were associated with the induction of α-amylase (EC 3.2.1.1) gene expression in aleurone of intact wheat (Triticum aestivum L. cv. Maris Huntsman) grains germinated at 25°C. The scutellum appeared to be the main site of de novo GA biosynthesis based on (1) the abundance of transcripts of a cloned wheat GA 20-oxidase. (2) the increase in content of GAs belonging to the early 13-hydroxylation GA pathway, and (3) the accumulation of ent-kaurene in grains imbibed in the presence of an ent-kaurene oxidase inhibitor. Again, the initiation of GA biosynthesis in the scutellum was closely associated with the induction of α-amylase gene expression in scutellar epithelium, although the two events may not have been causally linked. The embryo was required to be present for 36 h from the start of imbibition in order to induce α-amylase activity in aleurone, and the response could be replicated by low doses of GA1applied to de-embryonated grains. After-ripened wheat aleurone was relatively unresponsive to applied ABA in terms of suppression of GA-induced α-amylase production. Subtle differences were observed in the temporal pattern of α-amylase gene expression between intact germinated grains and de-embryonated grains challenged with GA1. It appears that endogenous GAs are an important component of the embryo stimulus initiating α-amylase gene expression in aleurone of germinating wheat grain, as originally proposed for barley. Their role in the synthesis of α-amylase in scutellar epithelium remains to be clarified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1997.tb03058.x
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  • 3
    Electronic Resource
    Electronic Resource
    Uptake and metabolism of 6-benzylaminopurine in shoot cultures of (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Shoots of Gerbera jamesonii Bolus, cultured in vitro were induced to multiply by the addition of 2.22 μM 6-benzylaminopurine (BAP) to the medium. Shoots growing in the presence of [14C]-BAP were harvested every 4 days and sub-divided into petioles plus apices, laminae and basal callus. The 3- and 9-glucosides, 9-riboside and a novel compound, the 9-ribosylglucoside were characterised as metabolites of BAP by mass spectrometry. The 9-riboside was the principal metabolite formed initially in petioles plus apicies and callus but further metabolism varied depending on tissue type. After 24 days the major metabolite in the callus was the 9-ribosylglucoside, with only trace amounts of the other metabolites present. In contrast, both 3-glucoside and 9-ribosylglucoside accumulated in petioles plus apices whereas the 3-glucoside accumulated in laminae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb08742.x
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of Abscisic Acid in the Xylem Sap of Willow ( Salix viminalis L.) by Gas–Liquid Chromatography (1968)
    [s.l.] : Nature Publishing Group
    Nature 220 (1968), S. 86-87 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Table 1. CONDITIONS FOR GLC OF ABSCISIC ACID Hewlett Packard, F and M 402 high efficiency Dual, 6' x g"i.d. glass, U shaped 1-5% Q.F.I coated on A.W.-D.M.C.S. 'Chromosorb W N2 40 ml. min-1 Gas Chromatography Columns Packing Carrier gas Flame ionization detector Injection temperature ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/220086a0
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  • 5
    Electronic Resource
    Electronic Resource
    Endogenous abscisic acid in relation to photoperiodically induced bud dormancy (1972)
    Springer
    Planta 106 (1972), S. 13-22 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The abscisic acid contents of birch, maple and sycamore plants growing under long and short photoperiods were measured by gas-liquid chromatography. No increase was observed in the abscisic acid content of extracts when plants were transferred to dormancy-inducing conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385469
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  • 6
    Electronic Resource
    Electronic Resource
    Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture (1988)
    Springer
    Planta 173 (1988), S. 110-116 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid in embryos ; Embryo development ; Hordeum (abscisic acid) ; Lectin ; Osmotic stress ; Triticum (abscisic acid)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Levels of endogenous abscisic acid (ABA) in immature wheat (Triticum aestivum cv. Timmo) and barley (Hordeum vulgare cv. Golden Promise) embryos have been determined by enzyme-linked immunosorbent assay. Embryos of both cereal species showed an increase in ABA content during development on the parent plant. Immature embryos were excised and cultured in vitro on nutrient media that led to precocious germination or on media containing 9% (w/v) mannitol that maintained their developmental arrest. Barley and wheat embryos responded to these culture conditions in an identical manner with respect to changes in morphology, fresh weight, protein and lectin content. However, in complete contrast, the ABA content of barley embryos increased by an order of magnitude during culture on mannitol, whereas that of wheat embryos showed no significant change. The results are discussed within the context of the role of ABA in the regulation of embryo development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394495
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  • 7
    Electronic Resource
    Electronic Resource
    The identification and quantitative analysis of abscisic acid in plant extracts by gas-liquid chromatography (1971)
    Springer
    Planta 96 (1971), S. 271-280 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary New techniques are described which permit the quantitative analysis of microgram quantities of abscisic acid in plant extracts by gas chromatography. Presumptive methyl abscisate peaks on gas chromatograms are positively identified by photosensitised isomerisation to methyl 2-trans-abscisate. Losses of abscisic acid during pre-purification are corrected by using 2-trans-abscisic acid as an internal standard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386942
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic map locations for orthologous Vp1 genes in wheat and rice (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 281-284 
    Details
    ISSN: 1432-2242
    Keywords: Key words Maize ; Vivipary ; Grain colour ; Dormancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Chromosome locations for gene orthologues of the dormancy-related maize transcription factor VIVIPAROUS-1, encoded by the Vp1 locus on maize chromosome 3, were determined in wheat (Triticum aestivum L.) and rice (Oryza sativa L.) via linkage to markers on existing molecular maps using a cDNA of a wheat Vp1 orthologue as a probe in genomic Southern analyses. Vp1-orthologous loci were detected on the long arms of wheat chromosomes 3A, 3B and 3D [Xlars10 (taVp1) loci] and rice chromosome 1 (osVp1), in line with previous evidence of synteny between these regions of the rice and wheat genomes and chromosome 3 of maize. The wheat loci mapped some 30 cM from the centromeres and some 30 cM proximal to the red grain (R) loci that control seed colour and coat-imposed dormancy. This unequivocal, genetic separation of the Vp1 and R loci may offer an opportunity for improving resistance to pre-harvest sprouting in wheat by combining the coat-imposed dormancy associated with red seed colour and true embryo dormancy regulated by Vp1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051069
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  • 9
    Electronic Resource
    Electronic Resource
    Phytohormone changes during storage root growth in Beta species (1984)
    Springer
    Plant growth regulation 2 (1984), S. 371-380 
    Details
    ISSN: 1573-5087
    Keywords: Beta vulgaris L. ; storage root development ; auxin ; cytokinin ; ABA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The physiological and morphological factors necessary for efficient accumulation of sucrose in sugar beet (Beta vulgaris L.) are considered in relation to potential uses of plant growth regulators to modify the anatomy of storage roots so as to increase sucrose content and yield. The percentage of sucrose in root fresh and dry matter is closely related to root structure. Sugar beet, mangold and chard are three sub-species of Beta vulgaris that differ considerably in their anatomy, assimilate partitioning, sucrose concentration and root dry matter yield. The concentrations of indole-3-acetic acid (IAA), abscisic acid (ABA) and cytokinins were measured during the growth of the storage root in each of these cultivars. Correlations were found between the phytohormone levels and the formation of secondary cambia and their subsequent cell division and expansion activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027296
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  • 10
    Electronic Resource
    Electronic Resource
    Growth retardant activity of paclobutrazol enantiomers in wheat seedlings (1994)
    Springer
    Plant growth regulation 15 (1994), S. 281-291 
    Details
    ISSN: 1573-5087
    Keywords: abscisic acid ; enantiomers ; gibberellin ; paclobutrazol ; sterols ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The resolved enantiomers of paclobutrazol appeared to have different primary modes of action as plant growth retardants in rht3 (tall) wheat seedlings. 2S,3S-Paclobutrazol reduced shoot growth more effectively than root growth, whereas the opposite was the case with the 2R,3R-enantiomer. Low concentrations (0.03–1.0 μM) of 2S,3S-paclobutrazol specifically inhibited gibberellin A1 (GA1) production in Rht3 (dwarf) seedlings without affecting shoot growth, confirming that inhibition of GA biosynthesis is the primary mode of action of this enantiomer. Reductions in shoot growth of rht3 (tall) wheat treated with 2S,3S-paclobutrazol were associated with reductions in GA1 content, an effect that could be reversed by gibberellic acid (GA3) application, showing that GAs are important regulators of light-grown shoot growth in wheat. The inhibition of root growth of wheat seedlings following treatment with 2R,3R-paclobutrazol was associated with a decline in de novo synthesis of major sterols, a decrease in stigmasterol: sitosterol ratio and an accumulation of the 14α-methyl sterol, obtusifoliol. Concentrations 〉3 μM 2S,3S-paclobutrazol also affected de novo sterol production in wheat roots, suggesting that root growth is more responsive to interference with sterol than GA biosynthesis. There was a decline in abscisic acid (ABA) content in Rht3 (dwarf) shoots treated with relatively high concentrations of 2S,3S-paclobutrazol but no effect with its optical isomer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029901
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