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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 14 (1974), S. 15-29 
    ISSN: 1432-0827
    Keywords: Osteocytes ; Osteoblasts ; Microtubules ; Microfilaments ; Intercellular Junctions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des cellules osseuses de calottes craniennes de jeunes souris sont étudiées au microscope électronique. Des microtubules sont visibles dans les ostéoblastes et les ostéocytes dans le corps cellulaire, mais non dans les prolongements de la cellule. Le cytoplasme de ces prolongements est rempli de faisceaux de microfilaments de 50 à 70 Å, parallèles à l'axe longitudinal du prolongement. Au point de rencontre de 2 prolongements, les membranes cellulaires forment une jonction étroite. Ces jonctions s'observent entre les ostéocytes, entre les ostéocytes et les ostéoblastes et entre les corps des ostéoblastes, à la surface cellulaire. Les prolongements cellulaires habituellement se rencontrent côte à côte, formant ainsi une jonction étroite étendue. Les jonctions entre ostéoblastes sont courtes et sont de type macula. A l'intérieur de l'os, peu d'espace extra-cellulaire est visible. La probabilité de transport intracellulaire est envisagée.
    Abstract: Zusammenfassung Calvarien-Knochenzellen junger Mäuse wurden im ultrastrukturellen Bereich untersucht. Es wurden in den Osteoblasten und Osteocyten der Zellkörper, nicht aber der Zellfortsätze, Mikrotubuli festgestellt. Dagegen ist das Cytoplasma der Zellfortsätze mit Bündeln von Mikrofasern von 50–70 Å gefüllt, welche parallel der Längsachse der Fortsätze angeordnet sind. An den Stellen, wo zwei Fortsätze aufeinandertreffen, bilden die Zellmembrane eine feste Verbindung. Diese Verbindungen werden zwischen Osteocyten, zwischen Osteocyten und Osteoblasten und zwischen Osteoblastenkörper auf der Zelloberfläche festgestellt. Die Zellfortsätze verbinden sich im allgemeinen entlang ihren Seiten, wodurch eine lange feste Bindung entsteht. Die Verbindungen zwischen Osteoblasten sind kurz und vermutlich punktförmig. Innerhalb des Knochens ist kaum ein extracellulärer Raum sichtbar. Die Wahrscheinlichkeit des intracellulären Transportes wird diskutiert.
    Notes: Abstract Bone cells of calvaria from young mice were studied at the ultrastructural level. Microtubules were demonstrated in both osteoblasts and osteocytes in the cell body, but not in the cell processes. Instead, the cytoplasm of cell processes is filled with bundles of 50 to 70 Å microfilaments, running parallel to the long axis of the process. Where two cell processes meet, the cell membranes form a tight junction. These junctions are found between osteocytes, between osteocytes and osteoblasts, and between bodies of osteoblasts on the cell surface. The cell processes usually meet side-to-side, thus forming an extended tight junction. The junctions between osteoblasts are short and are believed to be spot-like. Inside the bone scarcely any extracellular space is visible. The likelihood of intracellular transport is discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0827
    Keywords: Parathyroid hormone ; Osteoclasts ; Electron microscopy ; Morphometry ; Metaphysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effects of parathyroid hormone (PTH) on the size of the osteoclasts, nuclei, ruffled borders, and clear zones in long bones of thyroparathyroidectomized (TPTX) rats were quantitated as a function of time. These data were compared with the number of osteoclasts in the bone and with plasma calcium levels. A significant increase in the average size of the ruffled borders was demonstrated 30 min after injection of 50 U of purified bovine PTH, and of the clear zones 30–90 min after PTH. This was followed at 90 min by an increase in the average size of the cells. The sizes of ruffled borders and clear zones dropped sharply to control levels after 6 h, whereas the size of the cells remained elevated up to 12 h and returned to control values at 24 h. Plasma calcium levels were increased, but not significantly, between 30 min and 6 h. An increase in the number of osteoclasts was significant after 12 h. Removal of the parathyroid glands did not diminish the normal activity of osteoclasts. In animals with intact glands injection of 50 U of PTH did not cause a significant change in cell size or resorbing apparatus. It is concluded that PTH acts to rapidly stimulate the bone resorptive activity of osteoclasts and to cause a delayed increase in their number.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 34 (1982), S. 488-494 
    ISSN: 1432-0827
    Keywords: Bone resorption ; Macrophages ; Epithelioid cells ; Giant cells ; Subplasmalemmal linear densities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Implantation of mineral-containing bone fragments into calvarial defects in rats initiates a rapid and reproducible resorption of the bone matrix. After 7 days, a dense tissue develops with mononucleated as well as multinucleated cells surrounding and between the bone fragments. Electron microscopy revealed that these cells belong to the mononuclear phagocytic system; they were identified as macrophages, epithelioid cells, foreign body giant cells, and Langerhans cells. In addition to the common ultrastructural characteristics, these cells had electron-dense, focal specializations along their cell membrane with a coating on the exterior, corresponding to subplasmalemmal linear densities. Small, unidentified cells with electron-dense ground cytoplasm were often seen in close proximity to more differentiated cells. No halisteresis had occurred on the surfaces of the bone fragments. Indentations resembling Howship's lacunae were frequent; these contained mononucleated as well as multinucleated cells. Some surfaces were frayed and collagen fibers were exposed, but the cells apposed to these surfaces did not have ruffled borders as are seen in osteoclasts. Some bone fragments were broken up and cell processes had penetrated deep into the cracks, separating pieces of matrix. Small matrix particles were phagocytosed by macrophages, but not by epithelioid cells or giant cells. It appears that enzymes capable of degrading bone matrix components were secreted by the more differentiated cells of the mononuclear phagocytic system. They eroded the bone surface in a way reminiscent of osteoclastic bone resorption. They also entered the canaliculi to act from within the bone fragment, a process possible only in dead bone. We suggest a possible relationship of these cells with osteoclasts.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 9 (1972), S. 131-139 
    ISSN: 1432-0827
    Keywords: Cartilage ; Epiphyses ; Endoplasmic reticulum ; Golgi apparatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Une méthode de fixation des cellules cartilagineuses de la métaphyse est mise au point. Une meilleure préservation des chondrocytes applatis montre de petites vésicules qui semblent naître dans l'ergastoplasme et se trouvent en grande quantité au niveau de l'appareil de Golgi. Différents types de vacuoles sont visibles dans cet appareil, à savoir des ≪vacuoles intermédiaires≫ et de ≪larges vacuoles≫ contenant des inclusions. Il parait probable que les vésicules et vacuoles jouent un rôle spécifique dans le transport et la production de polysaccharides de protéines sulfatées.
    Abstract: Zusammenfassung Es wurde eine Methode zur Fixation der Knorpelzellen aus der Epiphysenplatte entwickelt. die besser erhaltenen abgeflachten Chondrocyten zeigen kleine Bläschen, welche aus dem groben endoplasmatischen Reticulum zu stammen scheinen und in großen Mengen im Golgi-Bereich vorliegen. Verschiedene Arten von Vacuolen können im Golgi-Apparat unterschieden werden, z.B. “intermediate vacuoles” und “large vacuoles”, wobei die letztgenannten gewöhnlich Einschlüsse enthalten. Es ist wahrscheinlich, daß die Bläschen sowie die Vacuolen eine spezifische Rolle beim Transport und der Bildung von sulfatierten Proteinpolysacchariden spielen.
    Notes: Abstract A method of fixation has been developed for the cartilage cells of the epiphyseal plate. Improved preservation of the flattened chondrocytes reveals small vesicles that seem to originate in the rough endoplasmic reticulum and are found in large amounts in the Golgi area. Different types of vacuoles can be distinguished in the Golgi complex, e.g. “intermediate vacuoles” and “large vacuoles”, the latter usually containing inclusions. It seems likely that the vesicles and vacuoles each play a specific role in the transport and production of sulfated protein polysaccharides.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 9 (1972), S. 140-151 
    ISSN: 1432-0827
    Keywords: Cartilage ; Epiphysis ; Cell ; Differentiation ; Endoplasmic reticulum ; Mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Une étude ultrastructurale de chondrocytes hypertrophiques est rendue possible par suite d'une meilleure méthode de fixation. Contrairement à l'opinion qui veut que les cellules hypertrophiques meurent, nos résultats suggèrent nettement une activité cellulaire allant du début de la croissance de la cellule au stade où la lacune s'ouvre. La séquence des altérations cellulaires est décrite, en allant de l'augmentation de l'ergastoplasme à la présence de ribosomes libres et de granules denses aux électrons dans les mitochondries. Les diverses fonctions possibles de la cellule sont décrites. Il est possible que les chondrocytes hypertrophiques se transforment en cellules osseuses.
    Abstract: Zusammenfassung Eine verbesserte Fixationsmethode ermöglichte die Untersuchung der Feinstruktur des hypertrophischen Chondrocyten. Im Gegensatz zur allgemeinen Annahme, daß die hypertrophischen Zellen absterben, weisen unsere Resultate deutlich darauf hin, daß die Zellaktivität vom Moment der Zellvergrößerung bis zum Stadium, in welchem die Lacunen aufbrechen, vorhanden ist. Der Ablauf der Veränderungen innerhalb der Zelle wurde beschrieben, wobei die hervorstechendsten Charakteristika die Zunahme des endoplasmatischen Reticulums, das Auftreten von vielen freien Ribosomen und das Vorkommen von elektronenmikroskopisch dichten Granula in den Mitochondrien waren. Die möglichen Funktionen der Zelle wurde besprochen. Es ist äußerst interessant, daß dadurch die Hypothese, wonach die hypertrophischen Chondrocyten schließlich zu Knochenzellen werden, unterstützt wird.
    Notes: Abstract A study of the ultrastructure of the hypertrophic chondrocyte has been made possible by an improved method of fixation. In contrast to the general belief that the hypertrophic cells die, our results strongly suggest cell activity from the beginning of enlargement of the cell up to the stage where the lacuna breaks open. The sequence of alterations within the cell have been described, with the increase of endoplasmic reticulum contents, the appearance of many free ribosomes and the occurrence of electron-dense granules in the mitochondria as the most prominent characteristics. The possible functions of the cell have been discussed. Most interesting is the support for the hypothesis that hypertrophic chondrocytes may ultimately become bone cells.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0827
    Keywords: 1,25(OH)2D3 ; PGE2 ; OAF ; PTH ; Osteoclasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effects of 1,25-dihydroxy vitamin D3 [1,25(OH)2D3], prostaglandin (PGE2), and osteoclast-activating factor (OAF) on the size of osteoclasts, nuclei, ruffled borders, and clear zones in cultured long bones of fetal rats were quantitated. In addition, the number of osteoclasts in the bones was counted and the release of calcium from the bone into the culture medium was determined. These data were compared with the corresponding effects of parathyroid hormone (PTH). All agents tested increased the size of the ruffled borders significantly after 3 h, the size of the clear zones after 12 h, and the size of the cells after 12–24 h. No important differences in sizes were noticed between the agents tested or between the agents and PTH. The number of osteoclasts was increased after 24 h of treatment with PTH, but not after the other agents. Calcium release was significantly increased for all agents between 12 and 24 h. It is concluded that bone resorption by 1,25(OH)2D3, OAF, and PGE2 is mediated primarily by increased activity of existing osteoclasts similar to PTH activation.
    Type of Medium: Electronic Resource
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