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  • 1
    Electronic Resource
    Electronic Resource
    The evolution of a family of short interspersed repeats in primate DNA (1981)
    Springer
    Journal of molecular evolution 17 (1981), S. 148-155 
    Details
    ISSN: 1432-1432
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Human DNA contains 300 nucleotide interspersed repeated sequences which mostly belong to a single family of sequences called the Alu family. This work examines the evolution of this family of sequences in primates. Bonnet monkey (Macaque radiata) DNA contains a predominant family of 300 nucleotide repeats which has nearly the same restriction map as the human Alu family and which hybridizes to human Alu family repeats under Southern blotting conditions. Prosimian (Galago crassicaudatus pangeniesis) DNA also contains a prominent group of 300 nucleotide long repeated sequences which does not have the same restriction sites as the human Alu family but which does hybridize to the human Alu family under reduced stringency conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01733908
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  • 2
    Electronic Resource
    Electronic Resource
    Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences (1980)
    [s.l.] : Nature Publishing Group
    Nature 284 (1980), S. 372-374 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In preparation for base sequencing, 300-nucleotide repeated sequences are liberated from renatured human DNA (Cot = 68) by S1 nuclease digestion4. These 300-nucleotide duplex fragments are presumed to have frayed single strand ends which would be unsuitable for base sequencing (Fig. 1a). To ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/284372a0
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  • 3
    Electronic Resource
    Electronic Resource
    Strong negative and positive regulatory elements contribute to the high-level fruit-specific expression of the tomato 2A11 gene (1991)
    Springer
    Plant molecular biology 17 (1991), S. 615-630 
    Details
    ISSN: 1573-5028
    Keywords: 2A11 gene ; tomato ; fruit-specific expression ; promoter ; regulatory elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fruit-specific expression of β-glucuronidase (GUS) activity was produced in transgenic tomato plants when the GUS-coding region was flanked by 5′ and 3′ regions of the tomato 2A11 gene. Deletion studies on the 5′ region revealed a number of strong regulatory elements involved in the proper expression of the 2A11 gene. A 4.0 kb and a 1.3 kb 5′ region can confer high-level fruit-specific GUS expression, while a 1.8 kb 5′ region produces no GUS activity in leaf or fruit tissue. Thus, a strong negative regulatory element is present in the region between 1324 bp and 1796 bp upstream of the 2A11 transcriptional start and a strong fruit-specific positive regulatory element is present more than 1.8 kb upstream of the transcriptional start site. The 1.8 kb promoter region can be activated by the upstream insertion of the CaMV 35S enhancer sequence, albeit not in a fruit-specific fashion. Substitution of the 3′ region of the 2A11 gene with a different 3′ region does not seem to affect GUS expression significantly, indicating a minor role, if any, for the 3′ region in the fruit-specific expression of the 2A11 gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037048
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  • 4
    Electronic Resource
    Electronic Resource
    A functional map of the fruit-specific promoter of the tomato 2A11 gene (1993)
    Springer
    Plant molecular biology 21 (1993), S. 625-640 
    Details
    ISSN: 1573-5028
    Keywords: tomato ; fruit specificity ; promoter ; trans-acting factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 5′ region of the fruit-specific tomato gene, 2A11, contains both positive and negative regulatory elements. We divided the 5′ promoter region of the 2A11 gene into small fragments, ranging in size from 211 to 634 bp and used these short DNA fragments in in vitro protein-binding studies. These studies revealed the presence of at least four fruit-specific and one leaf- and fruit-active protein-binding domains. These promoter fragments, as well as other overlapping fragments, were tested for their ability to enhance expression from a truncated heterologous promoter in transgenic plants. This analysis showed the presence of four fruit-specific and three general or leaf-active positive regulatory elements. Comparison of the results obtained with these two approaches allowed us to draw a functional map of the 2A11 promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014546
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato (1989)
    Springer
    Plant molecular biology 13 (1989), S. 639-651 
    Details
    ISSN: 1573-5028
    Keywords: cDNA clones ; fruit-specific expression ; in situ hybridization ; nucleotide sequence ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone corresponding to the 2A11 cDNA was isolated from a tomato genomic library. Sequence comparison of the cDNA clone with the genomic clone shows they are identical over the shared region with the genomic clone possessing a single large intron near the 5′ end of the message. The open reading frame of 2A11 would encode a sulfur-rich polypeptide 96 amino acids in length. The identity of the putative protein is unknown. In situ hybridization shows that the 2A11 message is found throughout the pericarp cells in a tomato fruit. In contrast, in situ hybridization of early ripening stages with a polygalacturonase probe shows higher mRNA levels in cells of the outer pericarp and cells surrounding the vascular regions of the pericarp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016019
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  • 6
    Electronic Resource
    Electronic Resource
    A microassay for detection of DNA and RNA in small numbers of plant cells (1985)
    Springer
    Plant molecular biology 5 (1985), S. 183-190 
    Details
    ISSN: 1573-5028
    Keywords: cauliflower mosaic virus ; microtechnique ; nucleic acid hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A microtechnique for the detection of DNA or RNA in small numbers of plant cells (1–50) has been developed using cauliflower mosaic virus (CaMV) infection of turnip as a model system. Both DNA and RNA extracted from 10 mesophyll protoplasts from CaMV-infected plants can be detected by hybridization using a radioactive probe made from cloned CaMV DNA (pCaMV10). No hybridization above background was detected in extracts of protoplasts from uninfected plants. At least 0.15 pg (11 000 molecules) of purified pCaMV10 DNA can be detected. This method is superior to existing ‘macro’ techniques for nucleic acid detection as smaller amounts of tissue are required and the detection is approximately 100-fold more sensitive. re]19850326 rv]19850530 ac]19850611
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015682
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  • 7
    Electronic Resource
    Electronic Resource
    Regulation of metallocarboxypeptidase inhibitor gene expression in tomato (1991)
    Springer
    Molecular genetics and genomics 228 (1991), S. 281-286 
    Details
    ISSN: 1617-4623
    Keywords: Tomato ; Fruit development ; Wound-inducible expression ; Post-transcriptional regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tomato fruits contain a metallocarboxypeptidase inhibitor (MCPI) the sequence of which has already been determined. Here we report the isolation of a tomato cDNA clone that encodes the mature MCPI protein as well as an N-terminal signal peptide for entry into the secretory system and an eight amino acid carboxyterminal extension. MCPI RNA is present at very high levels in anthesis stage ovaries and decreases quite rapidly during fruit development. MCPI protein accumulation reflects the pattern of MCPI RNA accumulation in fruit, consistent with a transcriptional control of MCPI gene activity. In leaves, the levels of MCPI RNA and protein are very low. Wounding of the leaves causes a dramatic (100-fold) increase in steady-state level of MCPI RNA without a concomitant increase in MCPI protein level suggesting a control at the post-transcriptional or translational level of gene expression. Genomic DNA blot hybridization data indicate that MCPI in tomato may be encoded by a single gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282477
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