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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 90 (1979), S. 783-787 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of gynecology and obstetrics 237 (1986), S. 117-126 
    ISSN: 1432-0711
    Keywords: PAPP-A ; Brush border membrane ; Trophoblast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Pregnancy-associated plasma protein-A (PAPP-A) has been shown to exert immunosuppressive effects both on complement and on lymphoblastogenesis. It was of interest to see whether this protein could bind to syncytiotrophoblast microvillous membranes since these represent the effective interface between fetal tissue and the immunocompetent mother. Placental subfractions were prepared according to established techniques. PAPP-A was purified from different sources (pregnancy serum and plasma, retroplacental serum, placental extracts) and labelled with radioactive iodine. It could be shown that radioactive PAPP-A, irrespective of its biological origin was primarily binding to brush border membrane preparations but that significant binding was also seen with plasma membrane preparations. The binding was specific since α2-macroglobulin (a structurally related protein to PAPP-A) was unable to displace bound radioactive PAPP-A. Scatchard plot representation of the data indicated that the affinity of PAPP-A for its binding site was of the same order of magnitude as reported for the insulin or GnRH receptors of the trophoblast (Ka 2 107 − 2.5 108 M−1). These results are in agreement with the immunohistochemical localization of PAPP-A on the trophoblast providing that the small binding to plasma membrane preparations is due to contamination.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of gynecology and obstetrics 247 (1990), S. 83-93 
    ISSN: 1432-0711
    Keywords: PP19 ; Concentration ; Body fluids ; Placental tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Placental protein 19 (PP19) is one of the new placental tissue proteins identified in extracts from human term placenta by Bohn and Winkler [1]. We measured the PP19 concentration in body fluids and placental tissue by radioimmunoassay; the minimum detectable dose of standard was 1.5 ng/ml. Although ethylene diamine tetraacetic acid (EDTA-2K) inhibited the immunoreaction between PP19 (225/242) and anti-PP19 antibody (632 ZA), the PP19 concentration did not differ between serum and heparin and sodium citrate plasmas. The serum PP19 concentration was increased by hemolysis. In blood cell fractions separated by the Ficoll-Paque/Macrodex method, polymorphonuclear leukocyte fraction contained the highest PP19 concentration. The circulating serum PP19 concentration was 4.5±1.1 ng/ml (mean ± standard deviation) in the proliferative phase (n=8) and 5.1±1.6 ng/ml in the secretory phase (n=7) for nonpregnant women, and 4.6±2.2 ng/ml from men (n=12). Seminal plasma (n=8) contained 212.2±99.7 ng/ml. The maternal serum PP19 concentration in 291 normal pregnancies increased from 6.2 ng/ml (median) at 6–7 weeks of gestation to 34.1 ng/ml at 38–39 weeks. The mean PP19 concentration was higher in amniotic fluid and retroplacental blood, but lower in umbilical cord blood than that in circulating maternal serum. In hydatidiform mole, vesicular fluid contained high PP19 concentration (1154.6±659.5 ng/ml), although these maternal serum concentration was not statistically higher than normal range. The chorionic villous trophoblast contained more PP19 than decidua, chorion, and amnion. These results suggest that PP19 has an extraplacental source, even though the chorionic villous trophoblast may be the main source throughout pregnancy.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary PP19, a new placental tissue protein, has α1-β1 electrophoretic mobility, a molecular weight of 36 500 and 3.9% carbohydrate. To study immunocytochemical PP19 localization in extravillous trophoblast, we obtained formalin-fixed specimens from extravillous tubal pregnancy at gestational weeks (GW) 7–9 (12 blocks); four early intrauterine pregnancies at GW 7–13 (12 blocks); four late pregnancies at GW 28–38 complicated with intramural uterine myoma, placenta increta and abruptio placenta (8 blocks); four invasive complete moles (9 blocks); and seven primary and metastatic gestational choriocarcinomas (12 blocks). Immunohistochemical staining was done for PP19, pregnancy-specific β1-glycoprotein (SP1) and human chorionic gonadotrophin (hCG) using the indirect-labeled antibody method [purified PP19 (Lot no. 225/242) and antibody against PP19 (Lot no. 632ZA) prepared by H. Bohn, antibodies against hCG (Behringwerke, Marburg, FRG) and SP1 (Dakopatts, Copenhagen, Denmark)]. In both early and late intrauterine pregnancies, the extravillous syncytiotrophoblastic cell (XST) showed positive staining for hCG and SP1 in the cytoplasm, as well as for PP19, which stained more intensively in the nucleus than in the cytoplasm. The three proteins were not seen in the evtravillous cytotrophoblastic cell (XCT) in the trophoblastic cell column and shell. The interstitial cytotrophoblast-like cell (ICT), which infiltrated into the decidua and myometrium, and their blood vessels, was immunoreactively positive for PP19 but negative for hCG and SP1 with the exception of SP1-positive ICT in the myometrium in late pregnancy. XST and ICT in the endosalpinx of tubal pregnancy stained for all three proteins. In invasive complete mole, XST stained for the three proteins, but ICT infiltrating into the decidua and myometrium stained more intensively for PP19 than for either hCG or SP1. XCT did not stain for the three proteins. Staining for the three proteins in gestational choriocarcinoma resembled that in invasive mole. By PP19 staining, XST and ICT infiltrating into surrounding tissue were clearly distinguishable from other cells of similar shape. PP19 staining thus can be a useful histochemical marker in assessing the cell viability of the trophoblastic tumor after chemotherapy.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 10 (1989), S. 51-64 
    ISSN: 0197-8462
    Keywords: power substation ; harmonic distortion ; ELF magnetic field ; calculation ; measurement ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: This paper presents a theoretical analysis of electromagnetic environments in power substations in service. A new analytical method is developed and applied to magnetic field calculations in 187-kV and 66-kV substations. Experimental results verify that the method offers a good prediction of the magnetic field profiles in the substations except for the areas where localized field sources exist. The analytical results show that the magnetic field strength depends to a great extent on how the load powers distribute on individual lines and buses, that the magnetic fields in substations have a three-dimensional characteristic, and that the predominant field component depends on the position of interest. Moreover, it is suggested that objects placed in substations are exposed not only to magnetic fields of high strength in comparison to those in houses or near the distribution networks but also to fields with several percent of harmonic distortion.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 11 (1990), S. 71-89 
    ISSN: 0197-8462
    Keywords: 3-D modeling ; induced electric field ; induced current ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A user-friendly, numerical program has been developed to permit the calculation of induced currents in modeled bodies of human and infrahuman subjects. The program is based on a charge-simulation method (CSM), and it takes into account the three-dimensional (3-D) character of the extremely-low-frequency (ELF) electric field and of the models to be exposed. The principle of the method is to simulate a 3-D object, for example, an animal model, by a combination of several parts (blocks) having simple geometric forms such as a sphere, a cylinder, or a cone. This approach permits easy preparation of input data on the dimensions of the blocks and their positions in a 3-D arrangement. Other input data, such as the coordinates of the contour points and the imaginary values of charges inside objects, which are necessary in the calculations by CSM, are produced automatically by selecting an appropriate “level” for each block, according to its importance. To simulate parts having irregular shapes, special blocks may be added. In one series of experiments, induced currents were calculated for a baboon model in various postures: standing upright, positioned on four legs, and sitting on the floor. Calculated currents. the total induced current in particular, agreed very well with experimental values. Local currents in parts of the baboon models were more variable, ranging from 5% to 17% of measured values in the case of induced currents in the head. Some problems with this method, such as the effect of the dimensions of blocks or the choice of block levels, are discussed.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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