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  • 1
    ISSN: 1432-1890
    Keywords: Key words Arbuscular mycorrhizal fungi ; Hyphal phosphorus uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Plant phosphorus uptake via external hyphae of arbuscular mycorrhizal fungi has been measured using compartmented systems where a hyphal compartment is separated from a rooting compartment by a fine mesh. By labelling the soil within the hyphal compartment with a radioactive phosphorus (P) isotope, hyphal uptake of P into the plant can be traced. The objective of this growth chamber study was to test two hyphal compartments of different design with respect to their suitabilities for measurement of hyphal P uptake. One hyphal compartment was simply a nylon mesh bag filled with 32P-labelled soil. The labelled soil in the other hyphal compartment was completely surrounded by an 8–10 mm layer of unlabelled soil that served as a buffer zone. Mycorrhizal and non-mycorrhizal subterranean clover plants were grown in pots with a centrally positioned hyphal compartment. Uptake of radioactive P by non-mycorrhizal control plants was 25% of that by mycorrhizal plants with the mesh bag but only 3% when including the buffer zone. Based on this good control of non-mycorrhizal P uptake from within the hyphal compartment and its greater ease of handling once produced, we judged the hyphal compartment including a buffer zone to be superior to the mesh bag.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Immunogenetics 50 (1999), S. 301-308 
    ISSN: 1432-1211
    Keywords: Key words T-cell receptors ; Variable region genes ; Evolution ; Phylogeny ; Diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The receptor of a T lymphocyte (TCR) recognizes nonself antigens in the company of major histocompatibility complex (MHC) molecules presented to it by the antigen-presenting cell. The variable region of TCR is encoded by either a concatenation of variable region (TCR-V), diversity region (TCR-D), and joining region (TCR-J) genes, or a concatenation of TCR-V and TCR-J genes. The TCR-V genes exist as a multigene family in vertebrate species. Here we study the evolutionary relationships of TCR-V genes from humans, sheep, cattle, rabbits, mice, and chicken. These six species can be classified into two groups according to the frequency of γδ T-cells in their peripheral T-cell populations. The "γδ low" group of species includes humans and mice, in which γδ T-cells constitute very limited portion of the T-cell population. The "γδ high" group includes sheep, cattle, rabbits, and chicken, in which γδ T-cells comprise up to 60% of the T-cell population. Here, we compiled TCR-V sequences from the six species and conducted a phylogenetic analysis. We identified various TCR-V gene subgroups based on the analysis. We found that humans and mice have representatives from nearly all of the subgroups identified, while other species have lost subgroups to different extent. Therefore, the γδ low species have a high degree of diversity of TCR-V genes, while γδ high species all have limited diversity of TCR-V genes. This pattern is similar to that found for immunoglobulin variable region (IGV) genes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Immunogenetics 48 (1998), S. 312-323 
    ISSN: 1432-1211
    Keywords: Key words Recombination ; Gene conversion ; Allele ; MHC ; HLA ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Some alleles of the major histocompatibility complex (MHC) genes have a reticulate pattern of evolution, probably resulting from the exchange of segments by gene conversion or recombination. Here we compare the extent and patterns of reticulate evolution among the classical class I and class II loci of the human MHC using the recently developed compatibility and partition matrix methods. A complex pattern is revealed with substantial differences among loci in the extent and pattern of reticulation. Extremely high levels of reticulation are observed at HLA-B and HLA-DPB1, high levels at HLA-A and HLA-DRB1, moderate levels at HLA-C and HLA-DQB1, and low levels at HLA-DQA1. The reticulate events are concentrated in the exons encoding the highly variable, peptide-binding domains, suggesting that the sequence combinations produced by these events are maintained by natural selection.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 16 (1993), S. 66-70 
    ISSN: 1432-0789
    Keywords: VA mycorrhiza ; Glomus intraradices ; Hyphal N transport ; Cucumis sativus ; 15N recovery ; Root compartment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Transport of N by hyphae of a vesicular-arbuscular mycorrhizal fungus was studied under controlled experimental conditions. The N source was applied to the soil as 15NH inf4 sup+ or 15NO inf3 sup- . Cucumis sativus was grown for 25 days, either alone or in symbiosis with Glomus intraradices, in containers with a hyphal compartment separated from the root compartment by a fine nylon mesh. Mineral N was then applied to the hyphal compartment as 15NH inf4 sup+ or 15NO inf3 sup- at 5 cm distance from the root compartment. Soil samples were taken from the hyphal compartment at 1, 3 and 5 cm distance from the root compartment at 7 and 12 days after labelling, and the concentration of mineral N in the samples was measured from 2 M KCl extracts. Mycorrhizal colonization did not affect plant dry weight. The recovery of 15N in mycorrhizal plants was 38 or 40%, respectively, when 15NH inf4 sup+ or 15NO inf3 sup- was applied. The corresponding values for non-mycorrhizal plants were 7 and 16%. The higher 15N recovery observed in mycorrhizal plants than in non-mycorrhizal plants suggests that hyphal transport of N from the applied 15N sources towards the host plant had occurred. The concentration of mineral N in the soil of hyphal compartments was considerably less in mycorrhizal treatments than in controls, indicating that the hyphae were able to deplete the soil for mineral N.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Most terrestrial plant species form associations with arbuscular mycorrhizal fungi (AMF) that transfer soil P to the plant via their external hyphae. The distribution of nutrients in soils is typically patchy (heterogeneous) but little is known about the ability of AMF to exploit P patches in soil. This was studied by growing symbioses of Linum usitatissimum and three AMF (Glomus intraradices, G. mosseae and Gigaspora margarita) in pots with two side-arms, which were accessible to hyphae, but not to roots. Soil in one side-arm was either unamended (P0) or enriched with P; simultaneous labelling of this soil with 32P revealed that G. intraradices responded to P enrichment both in terms of hyphal proliferation and P uptake, whereas the other AMF did not. Labelling with 33P of P0 soil in the other side arm revealed that the increased P uptake by G. intraradices from the P-enriched patch was paralleled by decreased P uptake by other parts of the mycelium. This is the first demonstration of variation in growth and nutrient uptake by an AMF as influenced by a localized P enrichment of the soil. The results are discussed in the context of functional diversity of AMF.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Soil Biology and Biochemistry 26 (1994), S. 1541-1547 
    ISSN: 0038-0717
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 15 (1993), S. 253-258 
    ISSN: 1432-0789
    Keywords: Bacterial biovolume ; Carbon budget ; Glomus fasciculatum ; 3H-thymidine labelling ; Pseudomonas spp. ; Vesicular-arbuscular mycorrhiza
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cucumber was grown in a partially sterilized sand-soil mixture with the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum or left uninoculated. Fresh soil extract was places in polyvinyl chloride tubes without propagules of mycorrhizal fungi. Root tips and root segments with adhering soil, bulk soil, and soil from unplanted tubes were sampled after 4 weeks. Samples were labelled with [3H]-thymidine and bacteria in different size classes were measured after staining by acridine orange. The presence of VAM decreased the rate of bacterial DNA synthesis, decreased the bacterial biomass, and changed the spatial pattern of bacterial growth compared to non-mycorrhizal cucumbers. The [3H]-thymidine incorporation was significantly higher on root tips in the top of tubes, and on root segments and bulk soil in the center of tubes on non-mycorrhizal plants compared to mycorrhizal plants. At the bottom of the tubes, the [3H]-thymidine incorporation was significantly higher on root tips of mycorrhizal plants. Correspondingly, the bacterial biovolumes of rods with dimension 0.28–0.40×1.1–1.6 μm, from the bulk soil in the center of tubes and from root segments in the center and top of tubes, and of cocci with a diameter of 0.55–0.78 μm in the bulk soil in the center of tubes, were significantly reduced by VAM fungi. The extremely high bacterial biomass (1–7 mg C g-1 dry weight soil) was significant reduced by mycorrhizal colonization on root segments and in bulk soil. The incorporation of [3H]-thymidine was around one order of magnitude lower compared to other rhizosphere measurements, probably because pseudomonads that did not incorporate [3H]-thymidine dominated the bacterial population. The VAM probably decreased the amount of plant root-derived organic matter available for bacterial growth, and increased bacterial spatial variability by competition. Thus VAM plants seem to be better adapted to compete with the saprophytic soil microflora for common nutrients, e.g., N and P, compared to non-mycorrhizal plants.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Planta 189 (1993), S. 191-200 
    ISSN: 1432-2048
    Keywords: Chlorophyll fluorescence ; Eucalyptus ; Leaf (orientation, light-absorption profile) ; Photoinhibition ; Photosynthetic capacity (intraleaf gradient)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gradients in photosynthetic capacity through the leaf affect the shape of the irradiance-response curve. These gradients in photosynthetic capacity were manipulated by restraining leaves in different orientations. The shape or curvature of the light-response curve can be defined by Θ, where Θ=0 is a rectangular hyperbola and Θ=1 is a Blackman curve. Horizontal leaves had the highest Θ values when their adaxial (top) surface was illuminated and lowest Θ value when their abaxial (bottom) surface was illuminated. Vertical leaves had intermediate Θ values that were similar for illumination from either direction, indicating that both surfaces had similar photosynthetic capacities. The photosynthetic capacity near each surface was probed by measuring the resistance to photoinhibition by 2000 μmol quanta · m −2·s −1 for 2 h followed by 15 min dark relaxation. Resistance to photoinhibition was consistent with the amount of direct sunlight exposure during growth. By measuring three light-response curves for a given leaf, illuminating the leaf from either the adaxial or abaxial surface or with the adaxial and abaxial surfaces illiminated equally, it was possible to infer gradients in the light absorption and photosynthetic capacity of the leaf using a ten-layer model. The gradient in light absorption was not as steep as expected and the photosynthetic capacity declined from the adaxial surface but increased again approaching the abaxial surface, the increase being more pronounced in vertical leaves. The modelled gradients were qualitatively similar for dorsiventral and isolateral leaves. The gradients in light absorption and photosynthetic capacity were not identical and this results (1) in curvilinear relationships between the quantum efficiency of PSII determined by chlorophyll fluorescence and the quantum efficiency of leaf photosynthesis and (2) in light-response curves that slowly reach saturation rather than being abruptly truncated. The Θ value for the photosynthetic light-response curve will remain a parameter that has to be derived empirically, in contrast to the maximum quantum yield and photosynthetic capacity. The curvature factor, Θ, depends on CO2 partial pressure and the interplay between the gradients in light absorption and photosynthetic capacity through the leaf which can change depending on the light environment during growth.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 226 (2000), S. 237-244 
    ISSN: 1573-5036
    Keywords: Arbuscular mycorrhizal fungi ; field studies ; hyphal phosphorus transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experimental systems for measuring nutrient transport by arbuscular mycorrhizal (AM) fungi in soil are described. The systems generally include two soil compartments that are separated by fine nylon mesh. Both roots and root-external hyphae grow in one compartment, but only hyphae are fine enough to grow through the mesh into the other compartment. Application of tracer isotopes to the soil of this hyphal compartment can be used to measure nutrient uptake by plants via AM fungal hyphae. Use of compartmented systems is discussed with particular reference to phosphorus, which is the mineral nutrient transported in the largest quantity by AM fungi. Laboratory and field applications of the compartmentation methodology are presented with emphasis on the functioning of native AM fungal communities. Advantages and limitations of the method are considered and future important research directions are discussed in this context.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-5036
    Keywords: Copper ; Dazomet ; Field experiment ; Leek ; Phosphorus ; Pretransplant inoculation ; VA mycorrhiza ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Leek plants were preinoculated with a mixed inoculum ofGlomus caledonium, Glomus fasciculatum andGlomus sp., and transplanted to Dazomet disinfected and untreated field plots of moderate P deficiency. Successive harvests were made until 99 days after transplanting. Preinoculated leeks attained marketable weights 25 days earlier than uninoculated leeks from untreated soil and their final dry matter yields were 5.7 and 1.5 times as high as those of uninoculated leeks from disinfected and untreated soil, respectively. Phosphorus concentration in preinoculated leeks remained highest for at least 22 and 75 days after transplanting in untreated and disinfected soil, respectively. Preinoculation had a similar, although smaller, influence on Cu and Zn concentrations. Infection levels produced by introduced and indigenous VA endophytes in leeks reached plateaus of 90% and 40%, respectively, 47 days after transplanting. It is concluded that VAM is essential to leeks grown in moderately P deficient soils, and the potential for inoculating seedlings in commercial leek production is discussed.
    Type of Medium: Electronic Resource
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