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  • 1
    Electronic Resource
    Electronic Resource
    Der p 2 isoallergens have different allergenicity, and quantification with 2-site ELISA using monoclonal antibodies is influenced by the isoallergens (2002)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 32 (2002), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Der p 2 isoallergens have been reported and the possibility of different allergenicity has also been suggested. In addition, the quantification with 2-site ELISA may be affected by the isoallergens.Objectives Two different recombinant Der p 2 (rDer p 2) isoallergens were compared in terms of human IgE responses and the reliability of quantification of them with two-site ELISA kits which use monoclonal antibodies (mAbs) as capture and detection of Der p 2.Methods Seven different Der p 2 cDNA from the cultured Dermatophagoides pteronyssinus (DP) were cloned and polymorphism in nine amino acid residues was found. Two different recombinant isoallergens (rDer p 2A and rDer p 2B) were expressed and compared to their human IgE immune responses by ELISA and the ELISA inhibition test with 23 sera of DP-allergic patients. The reliability of quantification of two different available 2-site ELISA kits, which used mAbs for capture and detection of Der p 2, was evaluated.Results The ELISA optical density of rDer p 2B-specific IgE (sIgE) was higher than that of rDer p 2A (P 〈 0.001). The ELISA inhibition curve of rDer p 2B sIgE in pool I sera (n = 5; high sIgE both to rDer p 2A and rDer p 2B) did not show any differences in the 50% inhibition concentration and maximum inhibitory percentage of rDer p 2A and rDer p 2B sIgE. However, with pool II sera (n = 5; markedly higher sIgE to rDer p 2B than rDer p 2A), the 50% inhibitory concentrations (10 µg/mL vs. 40 ng/mL) and maximum inhibitory percentage (61% vs. 99%) of rDer p 2B sIgE with the two recombinant isoallergens were quite different. rDer p 2B could be quantified with two different 2-site ELISA kits, but rDer p 2A was detected by only one kit.Conclusion We conclude that isoallergens of Der p 2 may have different IgE immune responses. Quantification of Der p 2 with 2-site ELISA kits that adopted mAbs, might be affected by the prevalent form of the isoallergens in reservoir dust.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2002.01421.x
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  • 2
    Electronic Resource
    Electronic Resource
    X-ray pole-figure analyses of YBa2Cu3O7−x thin film on SrTiO3(100) prepared by rf diode sputtering (1990)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 67 (1990), S. 7460-7466 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The structural properties of a high-temeprature superconducting textured YBa2Cu3O7−x thin film on a SrTiO3(100) substrate have been studied by reflection x-ray pole-figure methods, which can elucidate the degree of preferred orientations of the crystallites in polycrystalline materials and the relative orientations and lateral lattice alignments of the film with respect to the substrate. The specimen was fabricated on a SrTiO3(100) substrate by rf diode sputter deposition and subsequent furnace annealing in flowing oxygen. The film was found to be more preferentially a axis oriented than c axis oriented with a grain ratio of about 4:1. The a-axis-oriented grains are heavily twinned and the twinning structure is reflection about the (013) plane. The pole figures of the c-axis-oriented grains are consistent with the (110) reflection twinning structure. Nevertheless, the crystal axes of both types of oriented grains are aligned with those of the substrate. In addition, the interface matching of the film with respect to the substrate is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.345831
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  • 3
    Electronic Resource
    Electronic Resource
    Characteristics and surface analysis of ion beam deposition from binary boron platinum (Pt58B42) liquid-metal ion source (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 63 (1988), S. 878-886 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Characteristics of a eutectic boron platinum (Pt58B42) liquid-metal ion source (LMIS) were analyzed and investigated with a quadrupole mass spectrometer (QMS), Auger electron spectroscopy (AES), and Rutherford backscattering spectroscopy (RBS). The source characteristics can be explained by the hydrodynamic model, particularly for needle geometry LMIS. Surface analysis with RBS and AES indicated that more boron is produced in the ion beam than left in the liquid alloy reservoir and more droplets are produced with a 10-μm emitter tip radius, which reduced boron current in the beam. The source instability was associated with droplet formation. AES results show that substantial neutral ions were produced which was not detected by QMS. RBS results on the depleted residual alloy remaining on the carbon ribbon heater indicated that the cause of the alloy system's short lifetime (33 h) is due to a change in alloy stoichiometry to a higher platinum content. Three different emitter tip radii (2.5, 5, and 10 μm) made of graphite were used in the present investigation. Higher boron current and high stability during ion emission was recorded with 2.5-μm graphite emitter tip radius than with the 5- or 10-μm tip radii.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.340029
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  • 4
    Electronic Resource
    Electronic Resource
    Channeling study of the co-operative Jahn-Teller effect in nickel chromite single crystal by backscattering and resonant nuclear scattering of helium ions
    Amsterdam : Elsevier
    Nuclear Inst. and Methods in Physics Research, B 5 (1984), S. 20-24 
    Details
    ISSN: 0168-583X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-583X(84)90564-0
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  • 5
    Electronic Resource
    Electronic Resource
    Channeling study of epitaxial Al and Ag films on Si(111) substrates
    Amsterdam : Elsevier
    Nuclear Inst. and Methods in Physics Research, B 40-41 (1989), S. 817-822 
    Details
    ISSN: 0168-583X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-583X(89)90485-0
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  • 6
    Electronic Resource
    Electronic Resource
    Micromachining of polyimide films with focused ion beams
    Amsterdam : Elsevier
    Microelectronic Engineering 5 (1986), S. 461-462 
    Details
    ISSN: 0167-9317
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-9317(86)90077-8
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  • 7
    Electronic Resource
    Electronic Resource
    Ion-beam mixing to create thermoelectric properties in alloys
    Amsterdam : Elsevier
    Nuclear Instruments and Methods In Physics Research 209-210 (1983), S. 185-189 
    Details
    ISSN: 0167-5087
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-5087(83)90798-6
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  • 8
    Electronic Resource
    Electronic Resource
    Numerical solution of two-layer, two-dimensional tidal flow in a boundary-fitted orthogonal curvilinear co-ordinate system (1995)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 21 (1995), S. 1087-1107 
    Details
    ISSN: 0271-2091
    Keywords: two-layer ; finite difference ; time splitting ; orthogonal curvilinear co-ordinates ; grid ‘block’ ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A new two-layer, two-dimensional mathematical model employing a finite difference method based on numerically generated boundary-fitted orthogonal co-ordinates and a grid ‘block’ technique for unsteady boundary problems is developed which can be used to simulate flows with density stratification in a natural water-body with complicated topography. In the model the turbulent exchange across the interface is treated empirically and a time-splitting finite difference method with two fractional steps is employed to solve the governing equations. The model is calibrated and verified by comparing the computational results with data measured in Tolo Harbour, Hong Kong. The simulation results mimie the field measurements very closely. The computation shows that the model reproduces the two-layer, two-dimensional tidal flow with density stratification in Tolo Harbour very well. The computed velocity hodographs show that the tidal circulations at various positions in each layer have different patterns and that the features of the patterns are independent of the tidal type except for their scales. The computed Lagrangian pathlines show that the tidal excursion is dependent on the tidal type, especially in the inner harbour and side-coves.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650211106
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