ISSN:
1432-041X
Keywords:
F9 cells
;
Differentiation
;
Retinoic acid
;
mRNA
;
Cytokeratins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have identified a cDNA coding for the murine keratin D from a collection of clones representing F9 teratocarcinoma stem cell mRNA sequences. These sequences are synthesized specifically after the addition of retinoic acid and cAMP to the culture medium. The clone is 1,382 nucleotides long and contains the entire information for the active polypeptide, the complete 3′ end and most, if not all, of the 5′ non-coding region. The mRNA is found in hepatocytes, in PYS-2 cells (an endodermal cell line) and in differentiated (retinoic-acid-treated) F9 cells, but not in untreated F9 cells. The length of the mRNA is 1.4 kb, as estimated by Northern blot hybridization. Southern hybridization performed under very stringent conditions detects a single fragment hybridizing strongly with the cloned cDNA, suggesting that the mouse genome contains only one or very few copies of this gene. We present the first complete sequence of a keratin expressed in simple epithelia, i.e. keratin D, and discuss its structural features.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00376018
Permalink
