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  • 1
    Electronic Resource
    Electronic Resource
    Localization of monoamine oxidase B in human brain by autoradiographical use of11C-labelled L-deprenyl (1989)
    Springer
    Journal of neural transmission 77 (1989), S. 55-64 
    Details
    ISSN: 1435-1463
    Keywords: Cryosection ; autoradiography ; monoamine oxidase ; brain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 11C-labelled L-deprenyl in vitro autoradiography was used to study the regional distribution of MAO-B in human brain. 80 μm thick cryosections from two human brains, a 67 years old female and a 58 years old male, were taken on tape/paper and transferred on to a gelatinized glass plate. The sections were then incubated with 34 and 54 nM11C-L-deprenyl for 15 min and exposed to a film sensitive to high energy radiation for 2 hours. The autoradiograms obtained were analyzed by computerized densiotometry. High11C-deprenyl binding was found in the caudate nucleus, putamen, thalamus, substantia nigra, medial and lateral geniculate bodies, hippocampus and periaqueductal gray. Moderate to low binding was observed in cerebral cortex. Cerebral cortex and white matter showed the lowest binding. The autoradiographic technique described proved to be a fast and reliable method to investigate the topographic localization of MAO-B in large cryosections of human brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01255819
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Increased binding of3H-L-deprenyI in spinal cords from patients with amyotrophic lateral sclerosis as demonstrated by autoradiography (1992)
    Springer
    Journal of neural transmission 89 (1992), S. 111-122 
    Details
    ISSN: 1435-1463
    Keywords: MAO-B ; ALS ; deprenyl ; spinal cord
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present investigation has applied quantitative autoradiography and histochemistry to study the regional distribution of MAO-B and its relation to the number of cells in respective regions. L-deprenyl binds irreversibly and quantitatively to the B-form of monoamine oxidase, MAO, and is an ideal3H-ligand to measure the MAO-B enzyme protein in tissues by means of in vitro autoradiography. The investigation is performed on spinal sections from five controls and five cases with amyotrophic lateral sclerosis (ALS) on cervical, thoracic and lumbar level. The highest density of3H-L-deprenyl binding was found around the central canal (lamina X). MAO-B was markedly increased (up to 2.5 times of values in controls) specifically in regions of neurodegeneration e.g. motor neuron laminae and corticospinal tracts. There was a high correlation between glial cell count and3H-L-deprenyl binding with a relation indicating enhanced MAO-B protein in glial cells within areas of neurodegeneration. In contrast the increased microglial cell number in ALS did not show any correlation with3H-L-deprenyl binding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01245357
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Insulin-like growth factor-1 receptors in human spinal cord: changes in amyotrophic lateral sclerosis (1994)
    Springer
    Journal of neural transmission 97 (1994), S. 73-84 
    Details
    ISSN: 1435-1463
    Keywords: Amyotrophic lateral sclerosis ; human ; insulin-like growth factors ; insulin-like growth factor receptors ; motor neurons ; receptor autoradiography ; spinal cord
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Neurotrophic factors are important for neuronal survival and maintenance in the adult nervous system. The regional distribution of insulin-like growth factor-1 (IGF-1) receptors in human spinal cords from controls and amyotrophic lateral sclerosis (ALS) patients was studied by immunohistochemistry and quantitative autoradiography. When comparing125I-IGF-1 binding in the different spinal levels of normal spinal cord the same distribution pattern was found in which the binding was highest in the central canal 〉 dorsal horn 〉 ventral horn 〉 white matter. In the ALS cases although a general upregulation of IGF-1 receptors was observed throughout the spinal cord, significant increases were observed in the cervical and sacral segments compared to controls. IGF-1 receptor immunoreactivity showed a similar pattern to that for125I-IGF-1 binding, with immunoreactivity being found in the gray matter of the spinal cord and enhanced immunoreactivity occuring in ALS patients compared to controls. In agreement with the distribution of IGF-1 receptors, IGF-1 immunoreactivity was found within the gray matter of the spinal cord. The cartography of IGF-1 receptors in the normal spinal cord as well as the change of these receptors in diseased spinal cord may be of importance in future treatment strategies of ALS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01277964
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    Paper (German National Licenses)
    Fulltext
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