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  • 1
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 394-395 (May 2002), p. 341-344 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric nephrology 3 (1989), S. 386-390 
    ISSN: 1432-198X
    Keywords: P-fimbriae ; Vaccines ; Antibodies, cross-reactive ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To test for cross-protective capacity of two different P-fimbriae vaccines we vaccinated baboons with fimbriae purified from eitherEscherichia coli strain ER2 or strain JR1. The vaccinated animals showed elevated antibody titers to P-fimbriae from each of theE. coli strains used, suggesting cross-reactivity as was expected from the results of immunoprecipitation of the fimbriae. Enzyme-linked immunosorbent assay inhibition by heterologous P-fimbriae proved this to be true immunologic cross-reactivity.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric nephrology 3 (1989), S. 391-396 
    ISSN: 1432-198X
    Keywords: P-fimbriae ; Pyelonephritis, acute ; Escherichia coli ; Vaccines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Protection against acute pyelonephritis was induced by immunization of baboons with purified P-fimbriae ofEscherichia coli as vaccines. To test for cross-protective capacity of two different P-fimbriae vaccines we vaccinated baboons with P-fimbriae purified from eitherE. coli strain ER2 or strain JR1 and subsequently challenged the animals withE. coli strain JR1. All vaccinated animals showed elevated antibody titers to P-fimbriae from both of theE. coli strains used. Both vaccines tended to reduce the time of bacteriuria. They partially prevented pyelonephritis and protected against loss of renal function.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Urological research 23 (1995), S. 33-38 
    ISSN: 1434-0879
    Keywords: Pyelonephritis ; Lymphocytes ; Cytokines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We studied the cellular and humoral events which follow experimental acute pyelonephritis from P-fimbriated Escherichia coli to gain insight into the relationships among cells and specifically cytokines to determine how early events in untreated infection lead to renal damage. Cynomolgus (Macaca fascicularis) monkeys were studied after they were subjected to unilateral ureteral bacterial inoculation. We evaluated the blood for leukocytosis and studied lymphocyte subsets using flow cytometry and monoclonal antibodies to the subsets and serum, complement, cytokines and antibody titers. Interleukin-1, 2, and 6 and tumor necrosis factor (TNF) were assayed by enzyme-linked immunoadsorbent assay (ELISA), using monoclonal and polyclonal antibodies. Leukocytosis was marked and there were significant elevations in serum cytokines, interleukin-1α, 2 and 6 with only small changes in the level of TNF. Interleukin-2 levels were sustained and may have upregulated the homing receptor for virgin lymphocytes. The studies illustrated the unique relationship between cytokines and lymphocytes and the response to bacterial infection, showing that the inflammatory response is regulated not only by cytokine activity but also by lymphocyte activation.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Urological research 21 (1993), S. 71-74 
    ISSN: 1434-0879
    Keywords: Nonhuman primate ; Prostatitis ; Seminal fluid ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Patients with prostatistis have been found to have decreased prostatic fluid zinc (PF Zn). It is unclear whether changes of prostatitis are due to an inherent lack of Zn in some patients or whether the changes, including reduced PF Zn, are due to the infectious process itself. Six nonhuman primates were infected with 1×105 E. coli JR340, a clinical isolate known to cause prostatitis in the monkey. After infection, seminal fluid for culture and zinc assay was obtained by electroejaculation. Zinc was measured by colorimetric analysis. Blood counts, quantitative renal scans and urine cultures were also monitored. The animals were followed for 4 weeks and then sacrificed. The histopathology showed resolving prostatitis and fibrosis. Zinc levels dropped with acute infection, and variably recovered as the infection cleared. Controls showed slight variability with respect to PF Zn. It appears that PF Zn decreases as a result of the infectious process.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Infection 17 (1989), S. 401-404 
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Escherichia coli-Stämme mit P-und Typ-1-Fimbrien können bei weiblichen grünen Affen die Vagina besiedeln; aufsteigende Harnwegsinfektionen werden jedoch gehäuft nur von Stämmen verursacht, die P-Fimbrien tragen. Die Inokulation der Blase mit Typ-1-Fimbrien tragendenE. coli führte zu einer kurzzeitigen Blaseninfektion, P-Fimbrien tragende Stämme lösten hingegen eine akute Pyelonephritis aus, obwohl die Tiere keinen vesikoureteralen Reflux hatten. Im Gegensatz zu P-Fimbrien tragenden Stämmen lösten folglich Typ-1-Fimbrien tragendeE. coli in der Regel bei Tieren ohne Abwehrschwäche keine aufsteigende Infektion aus, obwohl sie sich in der Vagina ansiedelten.
    Notes: Summary Escherichia coli with both P and type 1 fimbriae caused vaginal colonization in the female green monkey, while only the P-fimbriated bacteria frequently caused ascending bladder infection. Bladder inoculation caused only short-lived bladder infection from type 1 fimbriatedE. coli, but those with P-fimbriae caused acute pyelonephritis even in the absence of vesicoureteral reflux. Thus, type 1 fimbriae ofE. coli, while causing vaginal colonization, did not often cause ascending infection in the non-compromised host as did P-fimbriated bacteria.
    Type of Medium: Electronic Resource
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