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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 170 (1998), S. 297-303 
    ISSN: 1432-072X
    Keywords: Key words Hyperthermophile ; Hydrogenase ; Oxidoreductase ; Ferredoxin ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Elemental sulfur reduction by the hyperthermophilic bacterium Thermotoga neapolitana provides an alternative to hydrogen evolution during fermentation. Electrons are transferred from reduced cofactors (ferredoxin and NADH) to sulfur by a series of unknown steps. One enzyme that may be involved is an NADH:methyl viologen oxidoreductase (NMOR), an activity that in other fermenting organisms is associated with NADH:ferredoxin oxidoreductase. We found that 83% of NMOR activity was contained in the pellet fraction of cell extracts subjected to ultracentrifugation. This pellet fraction, presumably containing cell membranes, was required for electron transfer to NAD+ from ferredoxin-dependent pyruvate oxidation. However, the NMOR activity in this fraction used neither Thermotoga nor clostridial ferredoxins as substrates. NMOR activity was also detected in aerobically prepared vesicles. By comparison with ATPase activities, NMOR was found primarily on the cytoplasmic face of these vesicles. During these studies, an extracytoplasmic hydrogenase activity was discovered. In contrast to the soluble hydrogenase, this hydrogenase activity was completely inhibited when intact cells were treated with cupric chloride and was present on the extracytoplasmic face of vescides. In contrast to a soluble hydrogenase reported in Thermotoga maritima, this activity was air-stable and was inhibited by low concentrations of nitrite.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1437-9813
    Keywords: Histology ; Suburpthelial-subureteric injection ; Collagen ; Mini-pig ; Vesicoureteric reflux
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Endoscopic correction of vesicoureteric reflux by subureteric injection has become an accepted method of treatment in selected cases. Its efficacy has been demonstrated in the treatment of well over 1,000 ureters. However, there are doubts as to the long-term safety of the commonly injected substance Teflon. Glutaraldehyde cross-linked bovine collagen, a biodegradable substance, was suggested as an alternative material for injection. The aim of this study was to evaluate the histological long-term behavior of glutaraldehyde cross-linked bovine collagen injected into the mini-pig bladder. One hundred and eight deposits of cross-linked collagen, each containing 0.2 ml (n = 36) or 0.6 ml (n = 72) collagen paste, were injected into the suburothelial space of the bladders of 24 mini-pigs. The histological behavior of the implants was studied at monthly intervals over a period of 12 months. After only 2 months a marked invasion of host fibroblasts and formation of endogenous type I and III collagen could be observed. Over 12 months the invasion of fibroblasts and the formation of new collagen increased dramatically. No migration of collagen particles into the local lymphatic system could be observed. A new staining technique (solophenyl red 3BL) was introduced to selectively demonstrate type I and III collagen fibres. In addition, the efficacy of the subureteric collagen injections could be demonstrated by successfully treating reflux that was iatrogenically induced in the mini-pig bladders.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 175 (1979), S. 31-36 
    ISSN: 1433-8580
    Keywords: Miniature pig ; Cardiac output ; Organ blood flow ; Miniaturschwein ; Herzminutenvolumen ; Organdurchblutung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 16 juvenilen Miniaturschweinen mit einem durchschnittlichen Körpergewicht von 3,08 kg wurde ein Herzminutenvolumen von 23 ml/100 g Körpergewicht mit der Farbstoffverdünnungsmethode gemessen; die prozentuale Verteilung des Herzzeitvolumens und die effektive Organdurchblutung wurden mit der Mikrosphärenmethode ermittelt. Bei 12 adulten Miniaturschweinen mit durchschnittlich 21,5 kg Körpergewicht wurde ein Herzminutenvolumen von 14 ml/100 g elektromagnetisch bestimmt. Die Werte für das Herzzeitvolumen und dessen prozentuale Verteilung stimmen mit denen des Menschen gut, mit denen anderer Laboratoriumstiere (Hund, Affe) nur zum Teil überein.
    Notes: Summary In 16 juvenile minipigs weighing 3.08 kg an average cardiac output of 23 ml/100 g body weight was measured with the dye-dilution method. The microsphere technique was used to assess distribution of cardiac output and organ blood flow. In 12 adult minipigs with a mean body weight of 21.5 kg the cardiac output of 14 ml/100 g body weight was measured with an electromagnetic flowmeter. The values of total systemic and organic blood flow correlated well with those of man and partly with those of other laboratory animals.
    Type of Medium: Electronic Resource
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  • 4
    Title: Research Review 1993
    Contributer: Fichtner, W. , Huang, Q. , Kaeslin, H. , Felber, N. , Aemmer, D. , ETH Zürich/Integrated Systems Laboratory , ETH Zürich/Microelectronics Design Center
    Year of publication: 1994
    Pages: 103 S.
    Type of Medium: Book
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  • 5
    Book
    Book
    Zürich :ETH
    Title: Research review 1991
    Author: Fichtner, W.
    Contributer: Kaeslin , Felber, N. , Aemmer, D. , ETH Zürich
    Publisher: Zürich :ETH
    Type of Medium: Book
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