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  • 1
    Electronic Resource
    Electronic Resource
    Cellular features in desmoid fibromatosis and well-differentiated fibrosarcomas (1975)
    Springer
    Virchows Archiv 369 (1975), S. 155-164 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the study presented here the ultrastructural cellular features of three desmoids and four well-differentiated fibrosarcomas were compared. Electron microscopically, the tumors were almost identical relative to qualitative traits. The majority of cells corresponded to fibroblast-like cells with certain morphological variations. Especially cells with characteristics of myofibroblasts must be emphasized, whereas cells with an organelle composition of classical fibroblasts were surprisingly seldom. Considering the relations of cells to each other and to vessels the prevailing origin of tumor cells from preexisting fibroblasts is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433241
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural cytology of human osteosarcoma cells (1978)
    Springer
    Virchows Archiv 381 (1978), S. 49-61 
    Details
    ISSN: 1432-2307
    Keywords: Osteosarcoma ; Ultrastructural cytology ; Histogenesis ; Cellular modifications
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cytology of 6 osteosarcomas was examined by electron microscopy. In keeping with the varied pattern of osteosarcomas seen by light microscopy several types of tumor cells could be differentiated: osteoblast-like, fibroblast-like, chondroblast-like, osteoclast-like and histiocyte-like cells. Moreover, atypical malignant mesenchymal cells and vascular spaces were present. The individual cytoplasmic organelles are not considered to be specific to particular types of cell as seen from the discussion of the significance of rough endoplasmic reticulum, microfilaments and lysosomes. Only examination of the composite pattern of subcellular organelles allows the differentiation of certain cell types. All tumor cells visible in osteosarcomas are considered as modifications of a transformed common progenitor cell. Because of the variegated cytological picture a multipotent mesenchymal cell rather than an osteoblastic cell is assumed to be the ancestor cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433485
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  • 3
    Electronic Resource
    Electronic Resource
    Cellular composition of the so-called dermatofibroma (Histiocytoma cutis) (1975)
    Springer
    Virchows Archiv 367 (1975), S. 325-336 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Hand 9 typischer Fälle von Dermatofibrom bzw. Histiocytoma cutis wurde mittels histochemischer, enzymhistochemischer und elektronenmikroskopischer Methoden die celluläre Zusammensetzung untersucht. Dabei waren Hinweise auf anabolische und katabolische Eigenschaften der Zellen zu erheben. Elektronenmikroskopisch ergab sich ein breites Spektrum mesenchymaler Zellformen. Neben definierten fibroblasten- und histiocytenähnlichen Zellelementen fand sich eine Zellart, die das morphologische Bild zahlenmäßig beherrschte und die durch besondere Merkmale, wie unregelmäßige Kernformen, reichlich rauhes endoplasmatisches Reticulum, freie Ribosomen, Filamentbündel mit einzelnen Verdichtungsbezirken, Mikropinocytosevesikel und ein basalmembranähnliches Material an der ZellaußenScite charakterisiert war. Gelegentlich konnte eine Anordnung der Faserbündel wie in der glatten Muskulatur nachgewiesen werden. Auf Grund dieser Befunde ist eine gewisse Ähnlichkeit zu sogenannten Myofibroblasten festzustellen. Die Vielgestaltigkeit des morphologischen Bildes weist auf eine multipotente Vorläuferzelle hin; die Möglichkeit, daß es sich dabei um Pericyten handelt, wird diskutiert.
    Notes: Summary 9 typical cases of dermatofibroma or histiocytoma cutis resp. were studied by the aid of histochemical, enzyme histochemical and electron microscopical methods to examine the cellular composition of these lesions. The results suggest an anabolic and katabolic function of cells. Electron microscopically a broad spectrum of patterns of mesenchymal cells was found. Besides defined fibroblast-like and histiocyte-like elements a cell type was detected which was characterized by particular traits, as irregularnuclear outline, abundant rough endoplasmic reticulum, free ribosomes, bundles of filaments with single dense zones, micropinocytotic vesicles and a basement membrane-like material on the outer cell surface. This cell type constitutes the majority of cells in dermatofibroma or histiocytoma cutis resp. In some cells an arrangement of filament bundles resembling that in smooth muscle could be seen. By reason of these findings a certain resemblance to the so-called myofibroblasts can be stated. The variegation of the morphological picture suggests a multipotent precursor cell; the possibility of an origin from pericytes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01239339
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  • 4
    Electronic Resource
    Electronic Resource
    Zur Pathogenese der senilen Amyloidose (1971)
    Springer
    Virchows Archiv 352 (1971), S. 209-218 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurden Untersuchungen zur Pathogenese der senilen Amyloidose an Herzen alter Menschen mittels histochemischer, polarisations- und fluorescenzmikroskopischer Methoden durchgeführt. Die Ablagerung von Amyloidfibrillen erfolgt gerichtet an präexistenten Kollagenfasern, wobei eine Vermehrung fasergebundener sulfatierter Mucopolysaccharide nachzuweisen ist. Im Verlaufe einer fortschreitenden Amyloidbildung kommt es zum sukzessiven Ersatz der Kollagenfilamente durch Amyloidfibrillen und einer weiteren Erhöhung des Mucopolysaccharidgehaltes. Schließlich sind in größeren Amyloiddepositen polarisationsoptisch keine Kollagenfasern mehr nachzuweisen, während die charakteristische Fluorescenzmetachromasie mit Acridinorange voll ausgebildet ist. Es wird gefolgert, daß dieser Vorgang bei der senilen Amyloidose im Rahmen des physiologischen Bindegewebsstoffwechsels abläuft und daß keine prinzipiell neuen Struktureinheiten gebildet werden, jedoch die Synthese eines anomalen Mucopolysaccharides vorliegt. Ursachen und ein Modell der senilen Amyloidose werden diskutiert.
    Notes: Summary Investigations on the pathogenesis of senile amyloidosis were carried out on the hearts of old men using histochemical, polarizing and fluoresence microscopic methods. The deposition of amyloid fibrils proceeds by orienting with pre-existent collagenous fibres, during which an increase in the number of fibre-bound sulfated mucopolysaccharides may be observed. During progressive formation of amyloid, the collagenous fibres are replaced successively by amyloid fibrils, and the mucopolysaccharides increase in number coincidently. Eventually nor collagenous fibres can be detected by polarizing microscopy in larger deposits; at this time the characteristic fluorescent metachromasia by acridine orange is completely developed. It is supposed that in senile amyloidosis these processes take place during the physiological metabolism of connective tissue and that principally no new structural units originate, although an abnormal mucopolysaccharide is produced. The causes of senile amyloidosis are discussed, and a model for it proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00600671
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  • 5
    Electronic Resource
    Electronic Resource
    The subcutaneous fascial analogue of myositis proliferans (1975)
    Springer
    Virchows Archiv 368 (1975), S. 361-371 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 2 Fälle einer in der Faszie lokalisierten Variante der Myositis proliferans wurden histologisch und elektronenmikroskopisch untersucht. Dabei zeigt sich eine weitgehende Ähnlichkeit zur echten, intramuskulär lokalisierten Myositis proliferans. Die elektronenmikroskopischen Befunde ergeben eine überwiegend histiozytäre Differenzierung der Zellen und stark aktivierte proliferierende Kapillaren und schließen einen myogenen Ursprung der charakteristischen Ganglienzell-ähnlichen Riesenzellen aus. Auf Grund der Feinstruktur erscheint eine traumatische Genese möglich, wobei die Zellen ihren Ausgang von multipotenten Gefäßwandzellen nehmen könnten. Die Beziehungen zur Myositis ossificans und Faszitis nodularis werden diskutiert.
    Notes: Summary Two cases of the so-called fascial analogue of myositis proliferans were investigated by histological and electron microscopic methods. It was found that the structure of the fascial variant corresponds almost completely to the true myositis proliferans localized within the musculature. The electron microscopic observations show a preponderantly histiocytic differentiation of the cells and strongly activated proliferating capillaries, and exclude a myogenic origin of the characteristic ganglion-like giant cells. Ultrastructurally a traumatic genesis appears possibly, the cells of the lesion could derive from multipotent cells of the microvasculature. The relations to myositis ossificans and fasciitis nodularis are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00432312
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  • 6
    Electronic Resource
    Electronic Resource
    Cellular differentiations and structural characteristics in nasopharyngeal angiofibromas (1976)
    Springer
    Virchows Archiv 371 (1976), S. 273-282 
    Details
    ISSN: 1432-2307
    Keywords: Nasopharyngeal angiofibroma ; Fibroblast ; Vascular cells ; Fibromatosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An electron-microscopic study of 9 nasopharyngeal angiofibromas was performed in order to elucidate the ultrastructural characteristics. Stromal fibroblasts and proliferating cells of the microvasculature were found. The stromal fibroblasts were subdivided into 3 different groups: (1) “classical” fibroblasts, (2) fibroblasts with histiocytelike features, and (3) fibroblasts with myoid features. By proliferation the cells of the capillary vessels change into stromal cells. A particular pattern of nuclei and dense intranuclear granules is only found in stromal fibroblasts. Consequently fibroblasts as well as cells of the microvasculature contribute to the pool of tumor cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433074
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  • 7
    Electronic Resource
    Electronic Resource
    Intercellular substances in Hodgkin's lymphomas (1978)
    Springer
    Virchows Archiv 380 (1978), S. 81-90 
    Details
    ISSN: 1432-2307
    Keywords: Hodgkin's lymphomas ; Intercellular substance ; Crossbanded structures ; Spiny collagen ; Macrophage-like cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 20 biopsy specimens, selected from lymph node biopsies of 100 untreated patients with Hodgkin's disease, were investigated electron microscopically to examine the formation of extracellular material at ultrastructural level. In the lymphocyte predominance type (2 cases) intercellular substance consists of a moderate amount of fine granular material. In nodular sclerosis (9 cases) different types of fibrillar structures can be distinguished: Typical collagen fibers including “spiny collagen”, fibrils of 80–100 A thickness without periodicity, and peculiar cross banded structures (dense bands: 400–600 A, periodicity of 1 000–1 300 A). In areas rich in fibrillar intercellular substance activated macrophage-like cells predominate. The mixed cellularity type (6 cases) shows small amounts of intercellular material. In places there is a peculiar rhythmic arrangement of this material, with a resemblance to the cross banded structures. Lymphocyte depleted cases (3 examples) also reveal homogenous, flocculent and cross banded intercellular material. The latter structures are rather abundant, esp. in areas of so-called hyalinosis. These different structural organizations of fibrillar and banded intercellular substances are considered to be the expression of a varying arrangement and deposition of collagen fibers and their precursors. The development of cross banded structures may be related to altered synthetic processes. The role of macrophage-like cells in the production of the fibrous material is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00432894
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  • 8
    Electronic Resource
    Electronic Resource
    Untersuchungen zum immunhistochemischen Verhalten der senilen Plaques des menschlichen Gehirnes (1970)
    Springer
    Virchows Archiv 351 (1970), S. 333-339 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die intensive Thioflavin-S-Fluorescenz seniler Plaques weist auf Amyloidablagerungen hin. Davon ausgehend wurden immunhistochemische Untersuchungen an 10 Gehirnen mittels Anti-Human-Gammaglobulin- und Anti-Human-Fibrin-Serum vorgenommen. Die Fälle wurden dem Obduktionsmaterial 40 physiologisch gealterter Menschen entnommen. 5–10% der senilen Plaques des Gehirnes ergeben zentral positive immunhistochemische Reaktionen, wogegen die periphere Zone weniger intensiv fluoresciert oder reaktionslos ist. Darüber hinaus zeigen Wandungen einiger meningealer und cerebraler Gefäße unabhängig vom Vorliegen einer drusigen Entartung (Scholz) positive Befunde. Die Mehrzahl der senilen Plaques kann daher keine Beziehungen zu Blutgefäßen aufweisen und muß folglich lokal im Hirngewebe entstehen. Die positiven immunhistochemischen Reaktionen sind auf eine Serumexsudation zurückzuführen. Die immunologische Genese der Plaques wird diskutiert, und es wird die Schlußfolgerung gezogen, daß die senilen Plaques eine besondere Form der senilen Amyloidose auf dem Boden zellulärer Regulationsstörungen darstellen.
    Notes: Summary Senile plaques showed an intensive fluorescence with thioflavine S caused by the deposition of amyloid. From these results fluorescence-immune histochemical studies of senile plaques with antihuman-gamma globuline serum and anti-human fibrin serum were carried out on ten brains. Forty brains were selected from autopsies of normally aged men without mental diseases. About 5–10% of senile plaques of each brain showed in the central cores positive immunohistochemical reactions whereas the fluorescence in their peripheral zones appeared less intensive or was totally absent. Furthermore, the walls of some meningeal and cerebral vessels were also positive with these methods, independent of the plaque-like degeneration of arteries and capillaries (Scholz). It is assumed that the majority of senile plaques has no relation to the blood vessels and, therefore, the plaques arise locally in brain tissue. The findings of immunhistochemical positive plaques are due to an exudation of serum. An immunological genesis of senile plaques is discussed; it is concluded that the senile plaque is a particular form of senile amyloidosis due to insufficient regulatory mechanisms of glia cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00547205
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  • 9
    Electronic Resource
    Electronic Resource
    Demonstration of orderly arranged acidic groups in amyloid by alcian blue (1974)
    Springer
    Histochemistry and cell biology 39 (1974), S. 163-169 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Variant amyloid types and, for comparison, growing cartilage were stained with alcian blue by different methods (alcian blue at controlled pH, critical electrolyte concentration method) and evaluated in the polarization microscope. Additionally, specific histochemical pretreatments were performed prior to staining and the effects to the alcian blue staining were studied. Amyloid and cartilage alcianophilic in light microscope showed a red color in polarized light. The studies revealed the existence of oriented arranged acid mucopolysaccharides in amyloid and cartilage responsible for the polarization optical phenomenon. In amyloid the arrangement of acid mucopolysaccharides perpendicular to the amyloid fiber axis is present very likely. The nature of the polarization optical phenomenon is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492045
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  • 10
    Electronic Resource
    Electronic Resource
    Simultaneous enzyme overlay membrane (EOM)-based in situ zymography and immunofluorescence technique reveals cathepsin B-like activity in a subset of tumour vessels (2000)
    Springer
    Histochemistry and cell biology 114 (2000), S. 63-68 
    Details
    ISSN: 1432-119X
    Keywords: Angioneogenesis Cathepsin B Enzyme overlay membrane In situ zymography Oral squamous cell carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The analysis of the invasion front of oral squamous cell carcinoma (OSCC) has revealed a fundamental invasion-associated remodelling of the extracellular matrix as the result of a complex regulated interplay of matrix synthesis and matrix degradation. Cysteine proteinases, in particular cathepsin B, are implicated in tumour invasion in vivo and in vitro and are thought to be important mediators of metastasis. An in situ zymographic assay based on enzyme overlay membranes (EOMs) was established to define the tissue localisation of cathepsin B activity in OSCC. Using confocal laser scanning microscopy we present a double-labelling method for the rapid and reproducible simultaneous detection of cathepsin B-like activity and cellular or extracellular antigens based on an EOM and immunofluorescence technique on frozen sections. Applying this method, cathepsin B-like activity was mainly found in vascular structures within the invasive front of OSCC. Therefore, the results suggest a particular pathogenic role of cathepsin B in tumour angioneogenesis. The method can simply be transferred to other enzymes and can be recommended for more extensive studies of proteolytic activity in human malignancies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180000159
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