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  • 1
    Electronic Resource
    Electronic Resource
    Cellular distribution of 04 antigen and galactocerebroside in primary cultures of human foetal spinal cord (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 340-344 
    Details
    ISSN: 1432-0533
    Keywords: Human spinal cord ; Oligodendrocytes precursors ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The distribution of cell-surface 04 antigen and galactocerebroside (GC) was examined by duallabel indirect immunofluorescence assays on live primary cultures of human spinal cord cells dissociated from 8–12 week-old foetal tissue. Oligodendrocytes expressing GC on their surface were present in the cultures at early time points, and all GC-positive cells were found to also express cell-surface 04 antigen. The 04 antigen was found additionally on a further population of GC-negative cells in the spinal cord cultures, which did not react with antibodies to glial fibrillary acidic protein (GFAP), and were distinct from neuronal cells and cell processes which stained with anti-neurofilament antibody. Previous studies in mouse neural cell cultures have shown that 04 antigen-positive cells are direct precursors to GC-bearing oligodendrocytes (Schachner et al. 1982). In the human spinal cord cultures, a rapid decline in the number of cells expressing GC and/or the 04 antigen to a value below 1% was observed during the first 3 days in vitro. The present studies indicate that synthesis of GC occurs in the human spinal cord many weeks before myelination commences in vivo and that GC-negative oligodendrocyte precursors are present simultaneously with more mature GC-positive cells. In addition, it would appear that complex humoral or cellular ingredients may be required for the long-term in vitro maintenance of viable human foetal oligodendrocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690838
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  • 2
    Electronic Resource
    Electronic Resource
    Human oligodendrocytes in dissociated cell culture (1989)
    Springer
    Acta neuropathologica 78 (1989), S. 317-324 
    Details
    ISSN: 1432-0533
    Keywords: Cell culture ; Human foetal spinal cord ; Oligodendrocytes ; Multiple sclerosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human oligodendrocytes have been successfully maintained in cell cultures for 14 weeks using a modification of a method used previously for animal brain cell cultures. Dissociated cell cultures from spinal cords of human foetuses of 10 to 20 weeks gestional age were investigated for up to 98 days. Oligodendrocytes were identified by monoclonal human antiserum specific for myelin-associated glycoprotein, by polyclonal rabbit antiserum against myelin basic protein, and by the mouse monoclonal antibody 16G1. Astrocytes were identified by polyclonal antibodies against glial fibrillary acidic protein. Immunocytochemical cell identification was corroborated by electron microscopy, by which glial cells were investigated both in situ and in culture. Immunocytochemical staining of myelin-associated glycoprotein showed specifically labelled oligodendrocytes on electron microscopy. The present study indicates that human oligodendrocytes, a putative target in demyelinating disease, can be studied in dissociated cell culture of human foetal spinal cord for several weeks in vitro under stable conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687762
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Constantin von Monakow’s formative years in Pfäfers (2000)
    Springer
    Journal of neurology 247 (2000), S. 200-205 
    Details
    ISSN: 1432-1459
    Keywords: Key words Neurological history ; Von Monakow ; Diaschisis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Constantin von Monakow was the first professor of neurology in Switzerland and founder of the Swiss Neurological Society and of the Swiss Archives of Neurology and Psychiatry. He gained worldwide reputation as a neuroscientist mainly through his monumental work on neuropathology and cortical localization. His concept of diaschisis has been verified only in recent years by modern network concepts and imaging techniques. The basis of his work was developed with ingenious animal experiments and careful clinico-pathological comparisons during his early years, which he spent as assistant physician in the psychiatric clinic at St. Pirminsberg in Pfäfers, Canton St. Gallen, Switzerland from 1878 to 1885.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004150050563
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of anterior horn lesions by MRI in central European tick-borne encephalomyelitis (1999)
    Springer
    Journal of neurology 246 (1999), S. 1169-1171 
    Details
    ISSN: 1432-1459
    Keywords: Key words European tick-borne encephalitis ; Magnetic resonance imaging ; Central nervous system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a case of central European tick-borne encephalitis with cervical myelitis presenting clinically as a lower motor neuron syndrome of the upper limbs with proximal asymmetrical pareses and atrophies. There were no sensory deficits nor signs of lesions of the spinal pathways or signs of encephalitis or meningitis. The affected motor fibers of the upper limbs were electrically inexcitable, but sensory findings were normal. Electromyography of the paralyzed muscles revealed pathological denervation activity without voluntary activation. The initial magnetic resonance imaging (MRI) showed a large hyperdense lesion in the anterior part of the cervical cord from C3 to T1. Despite the fact that MRI changes disappeared completely within 6 weeks the patient showed only little improvement in the paralyzed muscles after 6 months. To our knowledge, these MRI changes in patients with tick-borne encephalitis, consistent with an isolated anterior horn lesion, have never been reported previously. The course may have been aggravated by an initial antibiotic treatment with cephalosporins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004150050537
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    Paper (German National Licenses)
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