ISSN:
1523-5378
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The relationship between Helicobacter pylori infection and gastric carcinoma remains controversial, especially in the African setting where infection is common, while gastric cancer is perceived to be uncommon, the basis of the so called ‘African enigma’. This discrepancy between infection and the development of disease is commonly attributed to differences in host, environment and bacterial factors. Interest in the bacterial factors has focused on heterogeneity in the so-called ‘virulence genes’.〈section xml:id="abs1-3"〉〈title type="main"〉Aim.The aim of this prospective, case-controlled study was to establish whether H. pylori infection is significantly associated with gastric cancer and to investigate whether gastric cancer is associated with genotypically distinct (as it relates to the candidate virulence genes) organisms in this population.〈section xml:id="abs1-4"〉〈title type="main"〉Methods.Patients with histologically confirmed gastric cancer were matched with nonulcer dyspeptic controls for age (within 5 years), gender and ethnicity. Helicobacter pylori status was determined by RUT, histology, culture and serology (locally validated and used as default determinant of H. pylori status). Tumors were classified according to the Lauren classification. The ‘virulence genotype’ of 17 paired culture samples was determined by previously described and validated molecular techniques (cagA presence, vacA alleles, structure of the cag pathogenicity island and analysis of the iceA alleles). Categorical variables were analysed by the χ2 test.〈section xml:id="abs1-5"〉〈title type="main"〉Results.Forty-eight patients (median age 59 years) could be adequately matched to controls. 39/48 (81%) cases and 43/48 (90%) controls were H. pylori positive (NS). Significant differences in the virulence genotypes of infecting strains were noted: vacAs2-controls 24%, cases 0%, p 〈 .00001; vacAs1 present – cases 100%, controls 76%, p 〈 .05; cagA-3′-length 〉 650 bp – cases 47%, controls 0%, p 〈 .002; cag pathogenicity island intact – cases 82%, controls 43%, p 〈 .04; iceA1 – cases 53%, controls 6%, p 〈 .005. cagA was found in all subjects.〈section xml:id="abs1-6"〉〈title type="main"〉Conclusion.This study indicates that, in this African population at least, there is no difference in the prevalence of H. pylori infection when comparing gastric cancer cases with matched controls. However, the findings suggest that gastric cancer may be associated with infection by organisms that are genotypically different from those not associated with disease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1523-5378.2001.00044.x
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