ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
In this study, muscle-derived stem cell (MDSC)/Pluronics/polycaprolactone (PCL)microparticle hybrid mixture was prepared as a potential injectable urethral bulking agent for thetreatment of urinary incontinence. The MDSCs were isolated from the gastrocnemius muscles ofSD rats by a modified preplate method and characterized through FACS analysis using variousprimary antibodies (CD34, Sca-1, CD45 and desmin). The hybrid mixture was prepared by themixing of PCL microparticles (diameter, 100~200 μm) and MDSCs-containing thermo-sensitivePluronic (F127/F68 mixture) solution (4.5/5.5, w/v). The hybrid mixture was easily injectedthrough 18G needle into the body and stably remained in the applied site without initial volumedecrease, owing to a well-packed structure of PCL particles exhibited in the hybrid mixture. It wasobserved that the MDSCs were stably grown in the hybrid mixture without severe inflammation andimmune reaction. From the results, we recognized that the hybrid mixture can be a good candidateas an injectable bulking agent for the treatment of urinary incontinence, due to their goodinjectability, volume retention and biocompatibility
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.281.pdf
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