ISSN:
1573-2568
Keywords:
EPIDERMAL GROWTH FACTOR
;
TRANSFORMING GROWTHFACTOR-β1
;
CHRONIC REJECTION
;
SMALL BOWEL
;
TRANSPLANTATION
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Long-term survival of small bowel transplants ishampered by chronic rejection. Epidermal growth factor(EGF) and transforming growth factor β (TGF-β)have opposing, regulatory roles in normal intestinal physiology and may be involved in thepathogenesis of chronic intestinal rejection. Our aimwas to investigate the expression of EGF andTGF-β1 in chronically rejecting smallbowel transplants. Orthotopic small bowel transplantation was performed inthe allogeneic DA-to-AS rat combination; Cyclosporin wasadministered temporarily to prevent acute rejection.Controls were DA isografts and normal DA rats. PreproEGF and TGF-β1 geneexpression was evaluated by northern blot analysis ofthe ileum RNA and standardized againstglyceraldehyde-3-phosphate-dehydrogenase expression.Allografts demonstrated functional impairment and histological features of chronicrejection, whereas isografts appeared normal. Allograftsdemonstrated a significant reduction of EGF mRNA whencompared to DA isografts. No significant changes were detected in TGF-β1expression in either allogeneic or syngeneic grafts. Inconclusion, this study demonstrates reduced preproEGFand preserved TGF-β1 gene expression inchronically rejecting small bowel transplants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026659720191
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