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  • 1
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Preliminary studies in hematological patients have indicated that treatment with rhG-CSF reduces basophil releasabilityex vivo. We examined this phenomenon further, in allergic patients. Ten patients with grass pollen rhinoconjunctivitis were given rhG-CSF (5 μg/kg/day s.c.) for 5 days, and examined before and after treatment. Basophil counts increased from 5 to 19×109/l (P〈0.01). Total blood histamine increased from 80 to 160 μg/l (P〈0.01), corresponding to a decrease in average basophil histamine content from 1.5 to 0.81 pg/cell (P〈0.01). Isolated mononuclear cells showed a significantly decreased histamine release (HR) when stimulated with A23187 and grass. Whole blood experiments showed a similar decreased HR to grass and anti-IgE (P〈0.01). However, we found an increase in total blood histamine. We conclude that treatment with rhG-CSF (1) increases the number of circulating blood basophils, (2) reduces the average histamine content per basophil, and (3) reduces the basophil releasability. These findings could be due to the mobilization of immature basophils from the bone marrow.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: Keywords Endothelin-1 ; insulin secretion ; glucagon secretion ; flow cytometry ; alpha cell ; beta cell ; clonal cell line ; endothelin receptor ; gene expression.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. The circulating concentrations of endothelin-1 (ET-1), a peptide derived from endothelium, are increased in hypertension and diabetes. Endothelin-1 has recently been shown to be an insulinotropic agent. The mechanism of action of endothelin-1 on the endocrine pancreas has not yet been clarified. Methods. We investigated the action of endothelin-1 on the insulin secretion, the binding of 125I-ET-1 to beta cells as well as its effects on purified beta and non-beta cells from normal rats. The expression of endothelin receptors in alpha- and beta-cell lines and in normal rat islets was also studied. Results. First, we studied the effects of endothelin-1 on insulin secretion from beta-cell lines (INS-1, βTC3 and MIN6). At all endothelin-1 concentrations applied (1 pmol/l to 1 μmol/l) no change in insulin secretion was found. Ligand-binding experiments on βTC3 cells showed no specific binding of 125I-ET-1. A prominent expression of ETA-receptor mRNA in an alpha-cell line (αTC1.9) and in normal rat islets was found whereas no expression was found in INS-1 cells. No influence of endothelin-1(1 μmol/l) on insulin secretion stimulated by glucose was detected from purified beta cells. Endothelin-1-(100 nmol/l) increased, however, both insulin and glucagon secretion from a mixture of purified beta and non-beta cells indicating that alpha cells seem to have a key role for the action of ET-1 on insulin secretion. Conclusion/interpretation. The insulinotropic impact of endothelin-1 is not caused by a direct action on the beta cells but seems to be mediated by a paracrine action, probably secondary to enhanced release of glucagon from the endothelin receptor positive alpha cells. [Diabetologia (1999) 42: 1302–1307]
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 38 (1990), S. 453-459 
    ISSN: 1432-1041
    Keywords: Drug utilization ; breast feeding ; smoking ; alcohol ; puerperium ; pharmacoepidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary In a retrospective questionnaire survey of 885 women who had given birth 3–5 months before, fewer of those who were still breast-feeding at 4 months (n=645) were using drugs than those who had stopped breast-feeding before 4 months (n=240), during the 2 week period preceding registration. The average number of doses (Defined Daily Doses/1000 women/day) was 166 and 307, respectively, in that period. The number of doses taken was significantly associated with the use of oral contraceptive agents (p〈0.005) and young maternal age (p〈0.05). Most of the variation in drug use between breast-feeding and not breast-feeding mothers was probably due to the greater use of contraception by the latter. The number of drugs used per mother in the 4 month period seemed to be best predicted by her and her infant's disorders. Long-term medication in breast-feeding women included many drugs for which there is incomplete or no data about milk transfer, e.g. salbutamol, clemastine, dexchlorpheniramine, phenylpropanolamine, cromoglycate and levomepromazine. The disorders most extensively treated with drugs in this period were dyspepsia, haemorrhoids and inflammation of the breast. The finding that smoking was associated with early weaning and consumption of alcohol with prolonged breast-feeding calls for further investigation. More information on these drug and health issues to the breast-feeding mother is highly desirable.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 20 (2004), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Four management systems combining high and low livestock densities (0.7 and 1.4 livestock units ha−1) and different types of organic manure (slurry and straw based FYM) were applied to an organic dairy crop rotation (undersown barley – grass–clover – grass–clover – barley/pea – oats – fodder beet) between 1998 and 2001. The effects of the management systems on crop yields and nitrate leaching were measured. In all four years, nitrate leaching, as determined using ceramic suction cups, was higher in the three crops following ploughing of grass–clover than under the barley or grass–clover. Overall, no significant differences in nitrate leaching were observed between the management systems. However, the replacement of the winter wheat crop used in the earlier experimental period (1994–97) by spring oats with catch crops in both the preceding and succeeding winters reduced nitrate leaching compared with the earlier rotation. Increasing the livestock density, which increased manure application by c. 60 kg total N ha−1, increased crop yields by 7 and 9% on average for FYM and slurry, respectively. Yields were 3–5% lower where FYM was used instead of slurry. The experiment confirmed the overriding importance of grassland N management, particularly the cultivation of the ley, in organic dairy crop rotations.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 17 (2001), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. An increasing number of breeding sows is kept outdoors in Europe. Outdoor pig production has benefits in terms of animalwelfare but may have hidden costs through nutrient losses. We investigated the distribution of nutrients in sow paddocks and the consequence for losses and utilization in the succeeding crop. Significant correlation between soil inorganicNand the distance to feeding sites was observed after the paddocks had been used by lactating sows for 6 months (P〈0.01). Near to feeders inorganic N levels became extremely high whereas 30–40m from feeders some patches hadN levels in the topsoil corresponding to the levels in the reference area without sows. In the following spring only a minor part of inorganic N was still present in the top 0–40 cm. Similarly, extractable P and exchangeable K in topsoil were significantly affected by distance to feeders with the highest values close to the feeders (P〈0.001). In addition there were significant effects of the distance to huts with increasing nutrient content closer to huts. Although huge variations in dry matter production and nutrient content occurred in the succeeding potato crop, these were only weakly related to the distribution of nutrients (N, P and K) in the previous year, which explained 17%of the total variation in dry matter production. To increase nutrient efficiency in outdoor pig production a uniformdistribution of nutrients should be obtained by manipulating the excretory behaviour of the sows and stocking densities must be adjusted to locally acceptable nutrient surpluses.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 15 (1999), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. In dairy farming systems the risk of nitrate leaching is increased by mixed rotations (pasture/arable) and the use of organic manure. We investigated the effect of four organic farming systems with different livestock densities and different types of organic manure on crop yields, nitrate leaching and N balance in an organic dairy/crop rotation (barley–grass-clover–grass-clover–barley/pea–winter wheat–fodder beet) from 1994 to 1998. Nitrate concentrations in soil water extracted by ceramic suction cups ranged from below 1 mg NO3-N l−1 in 1st year grass-clover to 20–50 mg NO3-N l−1 in the winter following barley/pea and winter wheat. Peaks of high nitrate concentrations were observed in 2nd year grass-clover, probably due to urination by grazing cattle. Nitrate leaching was affected by climatic conditions (drainage volume), livestock density and time since ploughing in of grass-clover. No difference in nitrate leaching was observed between the use of slurry alone and farmyard manure from deep litter housing in combination with slurry. Increasing the total-N input to the rotation by 40 kg N ha−1 year−1 (from 0.9 to 1.4 livestock units ha−1) only increased leaching by 6 kg NO3-N ha−1. Nitrate leaching was highest in the second winter (after winter wheat) following ploughing in of the grass-clover (61 kg NO3-N ha−1). Leaching losses were lowest in 1st year grass-clover (20 kg NO3-N ha−1). Averaged over the four years, nitrate concentration in drainage water was 57 mg l−1. Minimizing leaching losses requires improved utilization of organic N accumulated in grazed grass-clover pastures. The N balance for the crop rotation as a whole indicated that accumulation of N in soil organic matter in the fields of these systems was small.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 21 (2005), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Nitrate leaching from crop rotations supporting organic grain production was investigated from 1997 to 2000 in a field experiment at three locations in Denmark on different soil types. Three experimental factors were included in the experiment in a factorial design: (1) proportion of N2-fixing crops in the rotation (crop rotation), (2) catch crop (with and without), and (3) manure (with and without). Three, four-course rotations were compared, two at each location. The nitrate leaching was measured using ceramic suction cells. Leaching losses from the crop rotation with grass–clover green manure and without catch crops were 104, 54 and 35 kg N ha−1 yr−1 on the coarse sand, the loamy sand, and the sandy loam, respectively. There was no effect of manure application or time of ploughing-in the grass–clover green manure crop on the accumulated nitrate leaching from the entire rotation. Catch crops reduced nitrate leaching significantly, by 30–38%, on the sandy soils. At all locations catch crops reduced the annual averaged nitrate concentration to meet drinking water quality standards in the crop rotation with green manure. On the coarse sand there was a time lag between the onset of drainage and the start of N-uptake by the catch crop.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cord blood cells were incubated (passively sensitized) with sera from 27 patients with previous systemic reactions to insect stings. Histamine release (HR) from these cells was measured following exposure to venom extracts at increasing concentrations. The aim was to see whether this parameter could predict more efficiently than RAST and skin test the outcome of a subsequent re-sting. Results showed that HR from passively sensitized cells tended to reflect skin sensitivity and specific IgE levels. If patients were not re-stung during the follow-up period, HR from the passively sensitized cells frequently decreased whereas an increase was seen (in 6/13) when using sera collected after re-sting. In conclusion HR from passively sensitized cord blood cells could not satisfactorily predict re-sting reactions in the serum donors.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Theoretical Biology 35 (1972), S. 473-487 
    ISSN: 0022-5193
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    The @International Journal Of Applied Radiation And Isotopes 27 (1976), S. 339 
    ISSN: 0020-708X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
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