ISSN:
1432-1130
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract. Conventional fluorescence methods are usually applied for the detection of fluorescent substances. A simple technique taking into account nonfluorescent substances has been introduced applying two different signals: (i) the linear or conventional fluorescence (CF) occurring at relatively short path-lengths of the exciting radiation in the fluids, and (ii) the multi-path saturation fluorescence (MPSF) originating at long path-lengths, as in multi-path reflection cells, where the radiation is fully absorbed by the fluid. A relation between the fluorescence signals and the absorption coefficients of fluorescent and nonfluorescent substances has been theoretically derived. An experiment has been performed with drinking water polluted by aromatic substances. The fluorescence ratio between CF and MPSF obtained has yielded the total absorption. Thus, it has been influenced by both fluorescent and nonfluorescent substances. In comparison to the conventional absorption technique, CF/MPSF has been characterized by high correlations to substance concentrations and has shown a much higher sensitivity. The method of CF/MPSF combines the advantage of fluorescence being very sensitive and of absorption spectrometry measuring the total absorption coefficient.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00012708
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