ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Infection of BSC-1 cells by SV40 brings about an increase of 7–11-fold in DNA polymerase activity, found in the nuclei and cytoplasm, respectively. The overall ratio between activities of DNA polymerase β (3.1S) and DNA polymerase α1 (5.5S) remains fairly constant throughout infection. However, there is a large increase in DNA polymerase α2 (7.1S) in the cytoplasm, and its appearance in the nuclei late in infection. The addition of 1M NaCl to infected cytoplasm, causes an aggregation of DNA polymerase α into a higher sedimenting form (9.8S), termed DNA polymerase α3. DNA polymerase α1, α2 and α3 are different molecular forms of the same enzyme, as can be seen by their similar inhibition by N-ethylmaleimide, heparin and NaCl. However, this new activity, α3, is stimulated by dithiothreitol to a greater extent at pH 9.30 than at pH 7.94. The conformational changes induced in DNA polymerase and its increase in activity during infection with SV40 are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01317870
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