ISSN:
1432-0428
Keywords:
Keywords Nitric oxide
;
nitrogen monoxide
;
insulin secretion
;
glucose
;
nitric oxide synthase
;
alpha cell
;
beta cell
;
delta cell.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Nitric oxide (nitrogen monoxide, NO) acts as a signal transducer in a variety of cells. In the present study rat pancreatic islets were perifused with physiologically relevant glucose concentrations in the presence or absence of various NO-modulating agents. Perifusion in the presence of 0.1–1 mmol/l of the NO synthase inhibitor, NG-monomethyl-L-arginine or of 10 μmol/l of the NO-scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO), resulted in an inhibition of the early phase of glucose-stimulated insulin secretion by 60–65 % and 46 %, respectively. Light- and electron-microscopic studies revealed that pancreatic islets constitutively express NO-synthase in alpha and delta cells, where it is confined to the secretory granules. Therefore, these data indicate that NO may be important in the signal transduction pathway of the early phase of glucose-stimulated insulin secretion. [Diabetologia (1998) 41: 292–299]
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001250050906
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