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  • 1
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In cell free extracts prepared from protoplasts of n-tetradecane grown cells of Candida tropicalis we have found an ATP and NAD+ dependent alkane-dehydrogenase, an alcohol-dehydrogenase, an aldehyde-dehydrogenase and acyl-CoA synthetases. The study of these enzymes and their regulation allows us to propose a scheme of degradation of n-decane.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 9 (1980), S. 45-51 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Lignin degrading bacteria were isolated directly by an enrichment culture technique using an industrial kraft lignin (Indulin AT) as the sole carbon source. The lignin degrading ability of these isolates was assayed in pure cultures. One strain (Aeromonas sp.) had degraded 98% of the lignin (1 g/l) after 5 days of incubation. Different genera have been identified including Corynebacterium, Agrobacterium, Pseudomonas, Aeromonas, but also Klebsiella and Enterobacter. These strains were also able to assimilate different phenolic compounds considered as lignin related simple monomers.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 121-125 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The biodegradability under aerobic conditions of volatile hydrocarbons (4–6 carbons) contained in gasoline and consisting of n-alkanes, iso-alkanes, cycloalkanes and alkenes, was investigated. Activated sludge was used as the reference microflora. The biodegradation test involved the degradation of the volatile fraction of gasoline in closed flasks under optimal conditions. The kinetics of biodegradation was monitored by CO2 production. Final degradation was determined by gas chromatographic analysis of all measurable hydrocarbons (12 compounds) in the mixture after sampling the headspace of the flasks. The degradation of individual hydrocarbons was also studied with the same methodology. When incubated individually, all hydrocarbons used as carbon sources, except 2,2-dimethylbutane and 2,3-dimethylbutane, were completely consumed in 30 days or less with different velocities and initial lag periods. When incubated together as constituents of the light gasoline fraction, all hydrocarbons were metabolised, often with higher velocities than for individual compounds. Cometabolism was involved in the degradation of dimethyl isoalkanes.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    International Dairy Journal 3 (1993), S. 117-127 
    ISSN: 0958-6946
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 42 (1971), S. 413-419 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In order to improve the production rate of l-lysine, a mutant of Corynebacterium glutamicum ATCC 21513 was cultivated in complex medium with gluconate and glucose as mixed carbon sources. In a batch culture, this strain was found to consume gluconate and glucose simultaneously. In continuous culture at dilution rates ranging from 0.2 h−1 to 0.25 h−1, the specific l-lysine production rate increased to 0.12 g g−1 h−1 from 0.1 g g−1 h−1, the rate obtained with glucose as the sole carbon source [Lee et al. (1995) Appl Microbiol Biotechnol 43:1019–1027]. It is notable that l-lysine production was observed at higher dilution rates than 0.4 h−1, which was not observed when glucose was the sole carbon source. The positive effect of gluconate was confirmed in the shift of the carbon source from glucose to gluconate. The metabolic transition, which has been characterized by decreased l-lysine production at the higher glucose uptake rates, was not observed when gluconate was added. These results demonstrate that the utilization of gluconate as a secondary carbon source improves the maximum l-lysine production rate in the threonine-limited continuous culture, probably by relieving the limiting factors in the lysine synthesis rate such as NADPH supply and/or phosphoenolpyruvate availability.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 1136-1141 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The activity of xenobiotic-degrading microorganisms is generally high in a biphasic aqueous/organic system. Therefore, the influence of interfacial area variation on kinetic parameters of Candida sp. growing on ethyl butyrate was evaluated. Interfacial areas of both aseptic and cultured biphasic systems were utilized. Substrate transport measurements in aseptic system (where the interface varied with the organic-phase fraction and agitator speed) showed that the substrate concentration in the aqueous phase was constant at different agitation speeds and decreased as the organic phase increased. Kinetic measurements of the cultured system showed that kinetic parameters vary as functions of their respective aseptic interfacial areas. Higher µmax and K i and lower K s values were obtained with larger interfacial areas. Measurements of the cultured system showed that the interfacial area increased as the biomass increased, and that about 50% of the biomass was attached to the interface as an interfacial biofilm at the end of the culture. Results suggest that the growth and selection of xenobiotic-degrading microorganisms in a biphasic aqueous/organic system should be evaluated mainly on the basis of the activity of adhering biomass (forming a biofilm) at the interfacial area rather than on substrate transport to the aqueous phase
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 40 (1993), S. 399-401 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract During the cheese-making process, water activity (aw) is one of the essential environmental parameters acting on bacterial growth and metabolic pathways. The influence of aw on Streptococcus diacetylactis growth and lactic acid production was studied. The specific growth rate was linearly related to water availability in the milk medium. The cell behaviour was quite different above and below aw=0.95, which can be considered a limiting value. Below this value, the lactic acid production reached 1.4–6.1 mg·g−1, whereas the specific productivity was 2.0–2.6 mg·10−10 cells·h−1. Changes in the consumption of lactose and amino acids during the different growth phases was completely modified by decreasing the water availability in the medium.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In the indirect impedancemetry technique, the CO2 produced during biological activity reacts with potassium hydroxide (KOH) solution, providing negative conductance variation. In this work, this technique was optimized, on a rapid automated bacterial impedance technique (RABIT) apparatus, developed by Don Whitley Sc. Ltd (UK). The KOH concentration and volume, as well as the temperature were tested. The dynamics of CO2 absorption and the ratio between the conductance variation and the amount of CO2 produced were examined. After injection of CO2 either directly in the KOH solution, or above the KOH solution, the best results were obtained with a KOH volume corresponding to immersion of the electrodes (0.7–1.2 ml), and with KOH concentrations of up to 7 g/1, although 5–6 g/l is preferred. Decrease of 280 μS/μmol CO2 was obtained at 27°C for a KOH concentration ranging from 0.5 to 8 g/1. All these results were slightly affected by temperature. However, it would be preferable for the CO2 produced to be bubbled directly into the KOH solution, in order to decrease the dynamic response of the system (gaseous transfer).
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  In the indirect impedancemetry technique, the CO2 produced during biological activity reacts with potassium hydroxide (KOH) solution, providing negative conductance variation. In this work, this technique was optimized, on a rapid automated bacterial impedance technique (RABIT) apparatus, developed by Don Whitley Sc. Ltd (UK). The KOH concentration and volume, as well as the temperature were tested. The dynamics of CO2 absorption and the ratio between the conductance variation and the amount of CO2 produced were examined. After injection of CO2 either directly in the KOH solution, or above the KOH solution, the best results were obtained with a KOH volume corresponding to immersion of the electrodes (0.7–1.2 ml), and with KOH concentrations of up to 7 g/l, although 5–6 g/l is preferred. Decrease of 280 μS/μmol CO2 was obtained at 27°C for a KOH concentration ranging from 0.5 to 8 g/l. All these results were slightly affected by temperature. However, it would be preferable for the CO2 produced to be bubbled directly into the KOH solution, in order to decrease the dynamic response of the system (gaseous transfer).
    Type of Medium: Electronic Resource
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