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  • 1
    Electronic Resource
    Electronic Resource
    Confocal theta microscope with three objective lenses (1994)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 65 (1994), S. 3367-3372 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A microscope using three water immersion objective lenses which realizes confocal, 4Pi-confocal and various confocal theta microscopies in fluorescence, transmission, scattered, and reflection mode is described. An argon-ion laser is the primary light source. A pulsed titanium-sapphire laser allows two-photon absorption fluorescence microscopy. The instrument has a predicted resolution of 100 nm along the illumination axis and a three-dimensional resolution of 5×106 nm3 for lenses each with a numerical aperture of 0.75. This is an improvement of an order of magnitude over a confocal fluorescence microscope using the same lens. Applications of the microscope range from observation of a sample at three different angles, to confocal theta fluorescence microscopy with multiphoton absorption. Since mounting and immersion media are identical, aberrations become negligible. The large working distance of 2 mm makes the instrument ideal for the observation of biological samples of up to 1.5 mm in diameter such as drosophila embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1144574
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A confocal fiber-coupled single-lens theta microscope (1998)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 69 (1998), S. 2956-2963 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A confocal theta microscope using a single water-immersion objective lens is described. The system is based on the Zeiss Axioplan universal microscope, such that the illumination light is coupled into, and the detected light out of, the microscope optics via optical fibers attached to the reflector slider of the microscope. Conventional wide-field, laser-scanning confocal, confocal theta, and 4Pi-confocal theta microscopy modes are available with the system. As the design can be easily adapted to other microscopes, objective lenses, and wavelengths, it allows confocal theta techniques to be implemented in many standard systems. The design constraints and specifications for the microscope are given, as well as a demonstration of its performance. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1149040
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    Paper (German National Licenses)
    Fulltext
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