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  • 1
    Electronic Resource
    Electronic Resource
    Membrane targeting and translocation of bacterial hydrogenases (2000)
    Springer
    Archives of microbiology 173 (2000), S. 319-324 
    Details
    ISSN: 1432-072X
    Keywords: Hydrogenase Metalloenzyme Folding Enzyme complex Signal peptide Twin-arginine Membrane targeting Cotranslocation Sec system Mtt/Tat system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Periplasmic or membrane-bound bacterial hydrogenases are generally composed of a small subunit and a large subunit. The small subunit contains a peculiar N-terminal twin-arginine signal peptide, whereas the large subunit lacks any known targeting signal for export. Genetic and biochemistry data support the assumption that the large subunit is cotranslocated with the small subunit across the cytoplasmic membrane. Indeed, the signal peptide carried by the small subunit directs both the small and the large subunits to the recently identified Mtt/Tat pathway, independently of the Sec machinery. In addition, the twin-arginine signal peptide of hydrogenase is capable of directing protein import into the thylakoidal lumen of chloroplasts via the homologous ΔpH-driven pathway, which is independent of the Sec machinery. Therefore, the translocation of hydrogenase shares characteristics with the ΔpH-driven import pathway in terms of Sec-independence and requirement for the twin-arginine signal peptide, and with protein import into peroxisomes in a "piggyback" fashion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030000144
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  • 2
    Electronic Resource
    Electronic Resource
    Improved Electrodeposition of CuIn〈sub〉1-x〈/sub〉Ga〈sub〉x〈/sub〉Se〈sub〉2〈/sub〉 Films (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 336-338 (Apr. 2007), p. 758-759 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: CuIn1-xGaxSe2(CIGS) precursor films are fabricated on Mo foil by coelectrodeposition. Theinfluence of the applied potential and the electrolyte additive in the process of electrodeposition arediscussed. The precursor films are annealed in Ar for a short time to synthesize the polycrystalline thinfilm. The annealed layers are only phase-pure CuIn0.7Ga0.3Se2 and show a good crystallinity
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.336-338.758.pdf
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  • 3
    Electronic Resource
    Electronic Resource
    Macro-Residual Stress in 304 Stainless Steel Coated with ZrO〈sub〉2〈/sub〉-CeO〈sub〉2〈/sub〉 Thin Films by Sol-Gel Process (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 336-338 (Apr. 2007), p. 2649-2651 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: X-ray diffraction (XRD) method to measure the residual stress existing in the metal substratesurface layer was introduced and the sol-gel ZrO2-CeO2 thin film was successfully prepared on SUS304stainless steel substrate by dip-coating process. The macro residual stress existing in metal substrate wasanalyzed by XRD. It turns out that the compressive stress existing in the metal substrate surface layerincreases with the increase of heat-treated temperature. Based on the above study, colored stainless steelsof high quality were prepared by sol-gel process
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.336-338.2649.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    Dynamic Recrystallization of Ferrite in Low Carbon Steels (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 558-559 (Oct. 2007), p. 617-622 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Dynamic recrystallization(DRX) of ferrite in low carbon steels was investigated by hotcompression tests at temperatures of 550 to 700oC at strain rates of 0.001 to 10s-1. The results indicatethat DRX of ferrite can occur in low carbon steels and lead to grain refinement. With increasingZener-Hollomon parameter Z, its mechanism changes from discontinuous dynamic recrystallizationto continuous dynamic recrystallization, the turning point is approximately at Z=1×1016s-1 for a lowcarbon steel with 0.171wt% C. The results also indicate that changing the minor constituents of thelow carbon steel from pearlite colonies to fine cementite particles has an effect on promoting DRX offerrite, and the increase of Mn content and the presence of tiny Nb precipitates have opposite effectsrespectively. However, all these changes are of benefit to the refinement of recrystallized grains
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/17/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.558-559.617.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    The hydC region contains a multi-cistronic operon (nik) involved in nickel transport in Escherichia coli
    Amsterdam : Elsevier
    Gene 107 (1991), S. 37-42 
    Details
    ISSN: 0378-1119
    Keywords: Gene cloning ; Southern blot ; cation transport ; chromosomal deletion ; fnr ; hydrogenase ; in vivo expression ; nikA ; nikB ; recombinant DNA ; traffic ATPase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90294-L
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  • 6
    Electronic Resource
    Electronic Resource
    Empirical pseudopotential calculations of Cd1−xMnxTe (1996)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 6939-6942 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Empirical pseudopotential calculations for the entire range of alloy concentrations of cubic Cd1−xMnxTe are presented. The atomic form factors have been deduced empirically by fitting the band structure to spectroscopic data available from the literature. The pseudopotential band structures indicate optical bowing may occur in the alloy Cd1−xMnxTe and have been used to determine the effective masses of the electron and light, and heavy holes, which for CdTe are in agreement with accepted values. The effective masses for Cd1−xMnxTe are given for the first time, and are expressed as first- and second-order polynomials in x. The implications of these results for spectroscopic experiments are discussed. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.361519
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  • 7
    Electronic Resource
    Electronic Resource
    The structural dependence of the effective mass and Luttinger parameters in semiconductor quantum wells (1997)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 82 (1997), S. 3414-3421 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A detailed comparison of the empirical pseudopotential method with single and multiple band calculations based on the envelope function and effective mass approximations are presented. It is shown that, in order to give agreement with the more rigorous microscopic approach of the pseudopotential method, structural dependent effective masses and Luttinger parameters must be invoked. The CdTe/Cd1−xMnxTe system has been employed as an example, and the first pseudopotential calculations of quantum wells and superlattices in this material are presented. It is shown that the electron, light- and heavy-hole effective masses tend towards twice their bulk values in the limit of narrow quantum wells. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.365657
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular cloning of the fdhF gene of Escherichia coli K-12 (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 34 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The fdhF gene of Escherichia coli, coding for at least one component of benzyl viologen-linked formate dehydrogenase (FDH-BV) activity, was isolated on a ColE1-fdhF hybrid plasmid from the Clarke and Carbon colony bank.Endonuclease restriction maps of this plasmid and its pBR322-subcloned derivative, pLW06, were constructed. Various hybrid plasmids were further obtained by deletion of endonuclease-cleaved fragments from pLW06 DNA. Their complementation pattern was analyzed after introduction into different fdhF mutant strains. The fdhF gene was shown to be located on a 5.5 kb BamHI-PvuII-DNA fragment, which restored FDH-BV activity to the wild-type level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01430.x
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  • 9
    Electronic Resource
    Electronic Resource
    Enzymatic and physiological properties of the tungsten-substituted molybdenum TMAO reductase from Escherichia coli (1999)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 32 (1999), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The trimethylamine N-oxide (TMAO) reductase of Escherichia coli is a molybdoenzyme that catalyses the reduction of the TMAO to trimethylamine (TMA) with a redox potential of + 130 mV. We have successfully substituted the molybdenum with tungsten and obtained an active tungsto-TMAO reductase. Kinetic studies revealed that the catalytic efficiency of the tungsto-substituted TMAO reductase (W-TorA) was increased significantly (twofold), although a decrease of about 50% in its kcat was found compared with the molybdo-TMAO reductase (Mo-TorA). W-TorA is more sensitive to high pH, is less sensitive to high NaCl concentration and is more heat resistant than Mo-TorA. Most importantly, the W-TorA becomes capable of reducing sulphoxides and supports the anaerobic growth of a bacterial host on these substrates. The evolutionary implication and mechanistic significance of the tungsten substitution are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01340.x
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  • 10
    Electronic Resource
    Electronic Resource
    The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 9 (1993), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The complete nucleotide sequence of the Escherichia coli nik locus, which has been suggested to encode the specific transport system for nickel, has been determined. It was found to contain five overlapping open reading frames that form a single transcription unit. Deduced amino acid sequence of the nik operon shows that its five gene products, NikA to NikE, are highly homologous to components of oligopeptide-and dipeptide-binding protein-dependent transport systems from several Gram-negative and Gram-positive species. NikA represents the periplasmic binding protein, NikB and NikC are similar to integral membrane components of periplasmic permeases, and NikD and NikE possess typical ATP-binding domains that suggest their energy coupling role to the transport process. Insertion mutations in nik genes totally abolished the nickel-containing hydrogenase activity under nickel limitation and markedly altered the rate of nickel transport. Taken together, these data support the notion that the nik operon encodes a typical periplasmic binding-protein-dependent transport system for nickel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1993.tb01247.x
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