ISSN:
1432-0568
Keywords:
Fetal cow
;
Brain development
;
Fetuin
;
Plasma proteins
;
Immunocytochemistry
;
Cross-reactivity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Fetuin, α2HS-glycoprotein (α2HS), α-fetoprotein (AFP) and albumin have been shown to be present in some regions of the neocortex in two early stages of development of the cow brain using PAP immunocytochemistry. In the pre-cortical plate stage fibres of the primordial plexiform layer stained positively for fetuin. No staining was seen for albumin but plasma and cerebrospinal fluid (CSF) were positive for α2HS and AFP. In the early cortical plate stage the strongest fetuin positive staining was seen in the earliest formed cells of the plate. α2HS staining was much less intense but similar in distribution. The possible role of fetuin, or related glycoproteins, in cortical plate differentiation is discussed. Staining for AFP and for albumin was seen mainly in the ventricular zone and marginal zone fibres, and had a similar distribution and intensity for both proteins. Plasma and CSF stained for all four proteins. Tests showed some cross-reactivity between fetuin and anti-α2HS and, to a much lesser extent, between antisera to AFP and albumin and antigens denatured by fixation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00309848
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