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  • 1
    Electronic Resource
    Electronic Resource
    Stem-loop structures at the 3' end of tobacco Rubisco large subunit mRNA
    Amsterdam : Elsevier
    Gene 94 (1990), S. 195-199 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; chloroplast rbcL ; expression in E. coli ; inverted repeat sequence ; mRNA stability ; transcriptional terminator
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(90)90387-7
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Production of fertile transgenic barley (Hordeum vulgare L.) plant using the hygromycin-resistance marker (1995)
    Springer
    Plant cell reports 14 (1995), S. 329-334 
    Details
    ISSN: 1432-203X
    Keywords: Barley ; Hordeum vulgare ; immature embryo ; particle bombardment ; particle gun ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile transgenic barley (Hordeum vulgare L.) plants were obtained by high velocity particle bombardment. The plasmid pBCl was used to deliver the selectable hph gene and reporter Gus gene into immature embryo. After the selection culture 18 hygromycin resistant plants were obtained. Samples for Southern hybridization and enzymatic Gus assay were obtained from 11 plants. Southern hybridization confirmed the presence of the hph gene in the 11 hygromycin resistant plants(T0). Enzymatic assay indicated that all the t0 plants that showed hph positive in Southern analysis possessed detectable amount of Gus activity. To date all the 11 t0 plants reached maturity and mature seeds were obtained Transmission of the hph gene to progeny(T1) of two independent t0 plants was confirmed by Southern hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232038
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Assessment of genetic diversity in a Morus germplasm collection using fluorescence-based AFLP markers (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 1049-1055 
    Details
    ISSN: 1432-2242
    Keywords: Key words Mulberry ; Germplasm ; Fluorescence-based AFLP ; Cluster analysis ; Genetic similarity ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To meet various breeding objectives and to conserve the existing genetic resources of mulberry for future use, the present study was undertaken to investigate the amount of genetic diversity and to establish the relationships between mulberry genotypes using fluorescence-based AFLP markers. Genetic diversity was estimated in 45 mulberry accessions from different eco-geographic regions of Japan and other parts of the world. Five primer combinations amplified an average of 110 AFLP markers per primer combination, ranging in size from 35 to 500 bp. A high degree of polymorphism was revealed by these combinations that ranged from 69.7 to 82.3% across all the genotypes studied. Several rare genotype-specific bands were also identified which could be effectively utilized to distinguish different genotypes. The wide range in genetic similarity coefficients (0.58–0.99) indicated that the mulberry germplasm collection represents a genetically diverse popu-lation. The phenetic dendrogram generated by the UPGMA method grouped 45 accessions into four major clusters, which was in agreement with the results from conventional methods. Clustering of some genotypes into strictly separate groups was not readily apparent and no clear interrelationships could be depicted, in spite of their different geographic origin. In addition, AFLP analysis provided sufficient polymorphism for DNA typing and contributed additional insights into the genetic structure of the mulberry germplasm. These results will help in the formulation of appropriate strategies for conservation and variety improvement in mulberry, for which little or no knowledge of genetic diversity is currently available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051579
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  • 4
    Electronic Resource
    Electronic Resource
    Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures (1998)
    Springer
    Plant cell, tissue and organ culture 53 (1998), S. 67-74 
    Details
    ISSN: 1573-5044
    Keywords: anther culture ; callus ; embryo culture ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant regeneration via tissue culture varies with the genotype and is an important factor in establishing cell selection and genetic transformation systems. To select genotypes – especially Japanese ones – with a high regeneration capability, we screened 107 wheat genotypes (78 domestic, 29 foreign) for callus induction and regeneration capability from anther and immature embryo cultures. For anther culture, 83 of 107 genotypes tested induced calli and 45 regenerated plants. Only 9 genotypes, however, produced green plants, 25 produced only albino plants, and 11 produced both green and albino plants. Glennson 81 was the highest in callus induction, followed by Orofen, Danchi–komugi and Chris. The genotypes with a relatively high regeneration capability were Framala 80 at 24% and Glennson 81 at 19%, these two genotypes produced only green plants. For immature embryo culture, 97 genotypes showed a 90% callus induction rate and 74 genotypes regenerated plants. Very few genotypes produced albino plants. The genotypes with a high regeneration capability were Genaro 81 at 90%, Chinese Spring at 80%, and Norin 75 at 75%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006023725640
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    Paper (German National Licenses)
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