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  • 1
    Electronic Resource
    Electronic Resource
    Changes in specific radioactivities of corn-leaf metabolites during photosynthesis in 14CO2 and 12CO2 at normal and low oxygen (1974)
    Springer
    Planta 120 (1974), S. 113-123 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 12CO2- and 14CO2-exchange of illuminated corn leaf discs were measured at normal (21%) and low (1%) oxygen. After periods of exposure to 14CO2 or to 14CO2 followed by 12CO2, the discs were killed and the specific activities of some metabolites were determined. At both O2 concentrations the specific activity of 3-PGA increased and decreased rapidly during the first 5 min of 14CO2-feeding or 12CO2-flushing but did not equilibrate with that of the CO2 in the assimilation chamber even after 15 min. The specific activity of aspartic acid also showed bimodal kinetics during both feeding and flushing. The specific activities of 3-phosphoglyceric acid (3-PGA), aspartic acid and alanine were higher at 1% O2 than at 21% O2, but glycine and serine were lower in specific activity at 1% O2. The results are in agreement with the proposed initial fixation of CO2 into C4-dicarboxylic acids and subsequent transfer of this carbon to 3-PGA. Indirect evidence supports the idea that at 21% O2, CO2 was produced by the corn leaf discs in the light and was refixed into C4-dicarboxylic acids. At 1% O2, the photorespiratory process could also have been active although the flux of carbon through the glycolate pathway was probably smaller than at 21% O2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384921
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  • 2
    Electronic Resource
    Electronic Resource
    Changes in specific radioactivity of sunflower leaf metabolites during photosynthesis in 14CO2 and 12CO2 at three concentrations of CO2 (1974)
    Springer
    Planta 120 (1974), S. 245-254 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sunflower (Helianthus annuus L.) leaf discs were exposed to 14CO2 or 14CO2 followed by 12CO2 at 21% O2 and three different CO2 concentrations. After intervals of up to 15 min, the specific activity of some photosynthetic intermediates was determined. At all CO2 concentrations, the specific activity of 3-phosphoglyceric acid (3-PGA) increased most rapidly and after 15 min of 14CO2 feeding was 92% (967 ppm CO2), 87% (400 ppm CO2) and 53% (115 ppm CO2) of CO2 supplied to the assimilation chamber. The specific activity of glycine, serine and the photorespiratory CO2 was similar at all CO2 concentrations, in aggreement with their proposed close metabolic relationship in the glycolate pathway. However, the kinetics of serine and glycine labelling suggested that serine was not totally derived from glycine. Because the specific activity of these glycolate-pathway intermediates was very differnet from that of 3-PGA at all CO2 concentrations, not all of the carbon traversing this pathway came directly from the Calvin cycle. The non-equilibration of the 3-PGA with the feeding gas reflects the recycling of C from the glycolate pathway into the photosynthetic reduction cycle. Measurements of the rates of CO2 evolution in the light and estimates of the C flux through the glycolate pathway suggest that the photorespiratory activity was high and similar at 115 ppm CO2 and 400 ppm CO2 but inhibited at 967 ppm CO2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390292
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Changes in specific radioactivities of sunflower leaf metabolites during photosynthesis in 14CO2 and 12CO2 at normal and low oxygen (1974)
    Springer
    Planta 120 (1974), S. 125-134 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sunflower (Helianthus annuus L.) leaf discs were exposed to 14CO2 or 14CO2 followed by 12CO2 in an open gas-exchange system with incoming gas of approximately 400 ppm CO2 and either 21% or 1% O2. The 14CO2 and 12CO2 gas-exchange of the leaf discs were measured, and the specific activities of several metabolites were determined after different lengths of time. The rate of CO2 efflux by the leaf discs was ca. 20% of the net photosynthetic rate at 21% O2 but no CO2 efflux could be detected at 1% O2. At both O2 concentrations the specific activity of 3-phosphoglyceric acid (3-PGA) increased and decreased rapidly for the first 5 min, and then more slowly during 14CO2 feeding and 12CO2 flushing. At 21% O2, glycine, serine and alanine changed more slowly in specific activity than 3-PGA and at 1% O2 their specific activities were much lower than at 21% O2. The results at both O2 concentrations indicated that the glycolate pathway compounds were not derived solely from Calvin-cycle intermediates. At 1% O2 the flux of carbon from the immediate fixation products was inhibited and serine was at least partially produced from a precursor of higher specific activity than glycine, although the glycolate pathway may have been active even at 1% O2. The difference between the specific activities of 3-PGA and the feeding gases could be explained by the recycling of C from the glycolate pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384922
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  • 4
    Electronic Resource
    Electronic Resource
    Inheritance of mitochondrial DNA in lentil (Lens culinaris Medik.) (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 206-210 
    Details
    ISSN: 1432-2242
    Keywords: Lens culinaris ; Mitochondrial DNA ; Maternal inheritance ; Biparental mitochondrial inheritance ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment analysis was used to examine the inheritance of lentil mitochondrial DNA (mtDNA) in F1 and F5 progeny from intrasubspecific (Lens culinaris ssp. culinaris) crosses and in F1 progeny from intersubspecific (Lens culinaris ssp. orientalis x L. culinaris ssp. culinaris) crosses. Southern blots of digested parental and progeny DNA were hybridized to heterologous maize mtDNA probes specific to coxI and atp6 genes. Two restriction fragment polymorphisms separated L.c. ssp. culinaris ‘Laird’ and ‘Eston’ from L.c. ssp. culinaris ‘ILL5588’, and one restriction fragment polymorphism distinguished L.c. ssp. culinaris ‘Laird’ and ‘Eston’ from L.c. ssp. orientalis ‘LO4’. Twelve of 13 f1 progeny and all F5 progeny from the intrasubspecific crosses, and all F1 progeny from intersubspecific crosses had only maternal mtDNA restriction fragments. One f1 plant from an ‘Eston’ x ‘ILL5588’ cross inherited mtDNA fragments from both parents. Nuclear DNA inheritance was biparental in all F1 progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225143
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  • 5
    Electronic Resource
    Electronic Resource
    Paternal plastid DNA can be inherited in lentil (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 607-610 
    Details
    ISSN: 1432-2242
    Keywords: Lens culinaris ; Chloroplast DNA Maternal plastid inheritance ; Biparental plastid inheritance ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment analysis was used to study the inheritance of chloroplast DNA (cpDNA) in F1 progeny from crosses between Lens culinaris ssp. orientalis and L. culinaris ssp. culinaris. Twenty-five combinations of 11 restriction enzymes and three heterologous probes from Petunia hybrida cpDNA were used to screen six accessions of L.c. culinaris and one accession of L. c. orientalis for restriction fragment length polymorphisms (RFLPs). No variation in cpDNA was observed within the subspecies L. c. culinaris, but the L. c. orientalis accession was unambiguously distinguished from all six L. c. culinaris accessions by two RFLPs. Of ten F1 progeny from L. c. orientalis x L. c. culinaris crosses, nine had only maternal cpDNA restriction fragments but one F1 plant inherited cpDNA fragments from both parents. Nuclear DNA inheritance was biparental in all ten F1 progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222122
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  • 6
    Electronic Resource
    Electronic Resource
    Non-destructive semi-quantitative determination of acetylene reduction in the field (1980)
    Springer
    Plant and soil 56 (1980), S. 335-340 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Field method ; Nitrogen fixation ; Non-destructive ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Direct injection of acetylene into soil around plant roots, followed by determination of ethylene/acetylene ratios in the soil atmosphere has been tested as a rapid, non-destructive method of estimating acetylene reducing activity. In pots of artificial media as well as in field soil, the ratios determined within 10 min. after injection were significantly correlated with the rates of acetylenedependent ethylene production in detached roots. The method may be useful in preliminary screening of large numbers of plant-bacteria combinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02205862
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    Paper (German National Licenses)
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