ISSN:
1573-8221
Keywords:
estrogens
;
phenobarbital
;
protein synthesis
;
microsomal cytochromes of the liver
;
ovariectomized rats
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The action of estradiol dipropionate (250 μg/kg) and phenobarbital (80 mg/kg), both separately and in combination (a single injection of estradiol dipropionate after administration of phenobarbital for 4 days), was studied on ovariectomized rats. Administration of phenobarbital or estradiol dipropionate was shown to increase the incorporation of phenylalanine-H3 in a cellfree protein-synthesizing system from liver microsomes of ovariectomized rats by 86 and 53% respectively. Combined administration of the estrogen and barbiturate was not followed by summation of that effect. Correlation was found between the increase in the rate of incorporation of phenylalanine-H3 in vitro and the increase in the concentration of cytochrome P450 in vivo in the microsomes of the liver cells after both separate and combined administration of the preparations. The role of phenobarbital as an activator of estradiol metabolism in the liver cells is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00799885
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