ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The conditions for an optimum formation of protoplasts from mycelium of Pencillium citrinum, which produces extracellular lipase, were examined. The best results were achieved using Novozyme 234 in combination with β-glucuronidase. Chitinase or β-glucuronidase alone were not able to produce protoplasts under the given conditions. For the regeneration frequency, the type of osmotic stabilizer was important. The highest regeneration frequency (84.6% protoplasts) was achieved by using 0.6 M KCl as an osmotic stabilizer. Some regenerants were more effective in producing lipase than the parent. In particular, strain no. P-8 showed a three times higher lipolytic activity than the parent strain, but this high enzyme activity was unstable after subculture.
Additional Material:
3 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370160217
Permalink
