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  • 1
    Electronic Resource
    Electronic Resource
    Hydrolysis of plant oils by means of lipase from Rhizopus nigricans (1991)
    Springer
    Bioprocess and biosystems engineering 7 (1991), S. 133-135 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The crude enzyme powder from Rhizopus nigricans was immobilized by sorption and subsequent cross-linking with glutaraldehyde on collagen and polytetrafluoroethylene (PTFE) membranes. Lipolytic membranes were applied to plant oils hydrolysis. The comparison of the two types of membranes by calculating the time required to obtain 1 mole of free fatty acids (FFA) from 1 m2 of membrane area, indicates that hydrophobic PTFE membrane is a better one in spite of the fact that the amount of protein sorbed on PTFE membrane is about three times smaller than that for collagen membrane. The hydrolysis of sunflower oil was the most efficient at the temperature of 37 °C and a pH of 7. At these conditions the specific activity after immobilization was about four times higher than that of the soluble enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369424
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Optimization of growth and hydrolytic enzymes production by Fusarium culmorum using response surface method (1998)
    Springer
    Bioprocess engineering 19 (1998), S. 229-232 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Production of hydrolytic enzymes by a phytopathogenic fungus Fusarium culmorum was investigated. The proteolytic activity was observed when the fungus was grown in the medium containing starch or soybean meal as a carbon source. The amylolytic and lipolytic activities were not found. Response surface modeling was applied to shake-flask culture of the fungus to determine the optimum concentration of carbon source and optimal culture time for growth and protease production. The results indicated that the maximum yield of protease production corresponded to the concentration of soybean meal of 1.4 g/ml and culture time of 4.5 days. The fungus growth depends on the concentration of carbon source in the medium whereas the enzyme production was also influenced by the culture time and interaction between these two variables.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00009013
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Hollow fiber modules as a tool for enzyme and cell immobilization (1989)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 467-472 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Owing to their properties, hollow fiber modules are attractive carriers for the immobilization of biocatalysts. Various systems and modes of operation are summarized and discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370090515
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    Paper (German National Licenses)
    Fulltext
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