ISSN:
1435-0130
Keywords:
Burn-Bacterial translocation
;
Wound colonization
;
S. aureus
;
Str. pyogenes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Bacterial translocation (BT) and release of endotoxin from the gut may contribute to septic complications in severely burned patients. In earlier experiments, it was shown in a burn model that BT exceeded the role of wound dissemination when burn wounds were colonized with Pseudomonas aeruginosa. Burn wound colonization with Pseudomonas aeruginosa also enhanced BT. It was concluded that endotoxin from gram-negative micro-organisms colonizing the burn wound appeared to play a role in the increased BT. In this study, the contribution of burn wound colonization with the gram-positive micro-organisms S. aureus and Str. pyogenes to BT and endotoxemia has been examined in Escherichia coli monoassociated mice with a 30% scald. The animals were sacrificed two days post-burn. The peritoneal cavity, the heart, one lung, the liver, the spleen and the cecum were cultured. Endotoxin in plasma was determined by means of the Limulus amoebocyte lysate assay. It appeared that dissemination of E. coli from the gut played a more important role than S. aureus or Str. pyogenes dissemination from the burn wound in this model. In only one of the 15 surviving Str. pyogenes contaminated animals dissemination had taken place from the burn wound. Dissemination of S. aureus was not seen in the S. aureus contaminated group. The incidence of E. coli translocation two days postburn, to the spleen (p〈0.05) and liver (p〈0.01) was enhanced in the Str. pyogenes group compares to the control group. Wound colonization by S. aureus did not increase the incidence of E. coli translocation. Endotoxemia was found in three of the 15 Str. pyogenes and in none of the S. aureus contaminated animals. In one of the 15 control animals, endotoxin was detected in plasma. These differences are not significant. The cause of the enhanced BT to the spleen and the liver in the Str. pyogenes contaminated group remains as yet unclear.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00212180
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