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  • 1
    Electronic Resource
    Electronic Resource
    Optimization of experimental procedures for fast atom bombardment mass spectrometry (1982)
    s.l. : American Chemical Society
    Analytical chemistry 54 (1982), S. 2362-2368 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00250a050
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  • 2
    Electronic Resource
    Electronic Resource
    Allozyme variation in Atlantic salmon from the British Isles: associations with geography and the environment (2005)
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Journal of fish biology 67 (2005), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Data on geographical variation in allele frequencies at enzyme coding loci in Atlantic salmon from the British Isles were collated from published and unpublished sources. Statistically significant differences in allele frequencies were found among samples both within and among river systems, suggesting that the Atlantic salmon in the British Isles is not a panmictic population and that even within major river systems it cannot be treated as a single genetic stock for fisheries management purposes. Although there was some evidence of regional differences in the frequency of some rare alleles, most single-locus variation did not show strong geographic patterns, with the exception of the AAT-4* locus at which allele frequencies had a significant latitudinal cline. There was some evidence for the existence of genetically-distinct celtic and boreal races of Atlantic salmon in the British Isles as previously has been suggested. Multiple regression analyses revealed associations between genetic variation and local environmental conditions (i.e. between variation at MEP-2* and both temperature and local river gradient), providing additional evidence for adaptive population divergence in the species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0022-1112.2005.00845.x
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  • 3
    Electronic Resource
    Electronic Resource
    A clinical, demographic and microbiologic study of ANUG patients in an urban dental school (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of clinical periodontology 14 (1987), S. 0 
    Details
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract 35 ANUG patients were examined and compared clinically and demographically. Plaque removed from ulcerated sites in 20 patients was cultured using quantitative anaerobic procedures and examined by electron and darkfield microscopy. Patients were classified as having ANUG when presenting with ulceration and necrosis of interproximal papillae, pain and bleeding. The clinical symptoms of fetid odor, pseudomembrane formation, lymphadenopathy and elevated body temperature were present in 97%, 85%, 61% and 39% of the ANUG patients, respectively. 83% of the patients were smokers. The ANUG patients demonstrated a lower average age (24 years) than the general clinic population (32 years). There was a slightly higher % of male (54%) than female (46%) and the % of Caucasian (51%) and black (49%) ANUG patients were almost equal. Cultural studies revealed that gram-negative rods were the predominant cultivable micro-organisms present in the plaque, representing 78.2% of the total recoverable count. Of these, nearly half were strict anaerobes with Bacteroides gingivalis and Fusobacterium nucleatum accounting for 7.8% and 3.4%. respectively. Anaerobic and facultative gram-positive cocci (15.5%), gram-negative cocci (3.5%) and gram-positive rods (2.8%) were also isolated. Microscopic analysis of the morphologic composition of plaque revealed that rods (43%) constituted the greatest % of the total microorganisms observed followed by spirochetes (30%) and cocci (27%). 8 distinct types of spirochetal periplasmic flagellar arrangement were observed by electron microscopy, the “2-4-2” periplasmic flagellar arrangement being most numerous.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-051X.1987.tb00976.x
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  • 4
    Electronic Resource
    Electronic Resource
    Development of microsatellite PCR typing methodology for the sea louse, Lepeophtheirus salmonis (Krøyer) (2000)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 31 (2000), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In order to develop a microsatellite typing system for Lepeophtheirus salmonis (Krøyer), a DNA preparation method for individual sea lice suitable for analysis by polymerase chain reaction (PCR) was designed, and the DNA sequences of 50 L. salmonis microsatellite elements were determined. The microsatellites were composed of 60% perfect, 25% imperfect, and 15% compound repeats. Based on the flanking DNA sequences, four microsatellite-PCR assays were optimized and used in a pilot study to analyse L. salmonis samples collected in Ireland, Norway and Scotland. Two of the microsatellite-PCR assays targeted polymorphic loci amplifying seven and 10 alleles respectively. The results showed that microsatellite-PCR typing could detect genetic variation both within and between the L. salmonis groups, and also was capable of amplifying group-specific alleles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.2000.00514.x
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  • 5
    Electronic Resource
    Electronic Resource
    Differential expression of Atlantic salmon thyrotropin β subunit mRNA and its cDNA sequence (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 54 (1999), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To study the regulation of the thyroid system, an Atlantic salmon Salmo salar cDNA clone was isolated for thyroid stimulating hormone (TSH) β subunit gene. A cDNA (866 bp) was isolated from an adult Atlantic salmon pituitary cDNA library, this clone was sequenced and shown to be highly conserved when compared to other teleost β TSH subunit sequences. The cDNA was used as a probe for Northern blot analysis of total pituitary RNA from the different life cycle stages of Atlantic salmon. Northern blot analysis demonstrated that β TSH mRNA is expressed at all life cycle stages studied, including parr, smolt, immature fish at sea and sexually mature male fish. Densitometry of Northern blots showed that sexually mature male salmon had low levels of salmon β TSH mRNA compared to non-mature fish. Stunts, fish performing poorly in salt water, were shown to have elevated levels of β TSH mRNA when compared to healthy fish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1999.tb02031.x
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  • 6
    Electronic Resource
    Electronic Resource
    Local and systemic production of immunoglobulins to periodontopathogens in periodontal disease (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 19 (1984), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1984.tb01323.x
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  • 7
    Electronic Resource
    Electronic Resource
    A modified diffusion apparatus for the growth of single crystals (1978)
    Copenhagen : International Union of Crystallography (IUCr)
    Applied crystallography online 11 (1978), S. 62-62 
    Details
    ISSN: 1600-5767
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0021889878012728
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  • 8
    Electronic Resource
    Electronic Resource
    A regulatory locus, Hdc-e, determines the response of mouse kidney histidine decarboxylase to estrogen (1984)
    Springer
    Biochemical genetics 22 (1984), S. 1037-1046 
    Details
    ISSN: 1573-4927
    Keywords: mouse ; kidney ; histidine decarboxylase ; regulatory gene ; estrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Levels of histidine decarboxylase (HDC; EC 4.1.1.22) activity in female mouse kidney are modulated by estrogen (administered as implanted pellets). In some inbred strains HDC activity is induced by estrogen, while in others the enzyme is repressed. Immunoprecipitation with an anti-fetal rat HDC antiserum has shown that induction and repression of HDC levels are due to changes in enzyme concentration. Segregation analysis has identified a single additively inherited regulatory locus, Hdc-e, which determines the response to estrogen. The allele Hdc-e b (C57BL/10) determines induction, and the allele Hdc-e d (DBA/2) determines repression. Preliminary evidence indicates cosegregation of Hdc-e alleles with alleles of another regulatory locus, Hdc-c (determining kidney HDC concentration), and therefore putative linkage of Hdc-e with the HDC gene complex on chromosome 2. This is the first report of a mammalian regulatory gene controlling two opposite mechanisms, induction and repression in response to a single effector.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499630
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  • 9
    Electronic Resource
    Electronic Resource
    A structural gene (Hdc-s) for mouse kidney histidine decarboxylase (1984)
    Springer
    Biochemical genetics 22 (1984), S. 645-656 
    Details
    ISSN: 1573-4927
    Keywords: mouse ; kidney ; histidine decarboxylase ; structural gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The concentration of mouse kidney histidine decarboxylase (HDC) is modulated by estrogen, testosterone, and thyroxine in a tissue-specific manner. Variation in HDC levels between strains of mice can be used to investigate the genetic regulation of (i) enzyme structure, (ii) tissue specific expression, and (iii) induction and repression by hormones. Variation in the structure of HDC between different inbred strains of mice affecting its K m for the cofactor pyridoxal-5′-phosphate (PLP) and its heat stability has been discovered. The alternative phenotypes are additively inherited in crosses and the heat stability difference is due to alleles of a single structural gene, Hdc-s, which segregate among the BXD and BXH recombinant inbred strains. The allele Hdc-s b determines the heat-stable phenotype (C57BL substrains), and the allele Hdc-s d the heat-labile phenotype (DBA/2 and C3H/He strains). The alleles of the structural gene cosegregate with alleles of a regulatory gene previously named Hdc (determining kidney enzyme concentration); there were no recombinants among 38 RI strains. Therefore the two loci are less than 0.685 cM apart and comprise part of the HDC gene complex, [Hdc], on chromosome 2 of the mouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00485850
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  • 10
    Electronic Resource
    Electronic Resource
    Histidine decarboxylase phenotypes of inbred mouse strains: A regulatory locus (Hdc) determines kidney enzyme concentration (1984)
    Springer
    Biochemical genetics 22 (1984), S. 305-322 
    Details
    ISSN: 1573-4927
    Keywords: mouse ; kidney ; histidine decarboxylase ; regulatory gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mouse kidney histidine decarboxylase (HDC) provides a model system to study genetic control of a hormone-regulated enzyme (inducible by estrogen and thyroxine; repressible by testosterone). Five major HDC phenotypes scored on the basis of (i) enzyme activity and (ii) the difference in activity between the sexes (females usually higher than males) have been discovered by screening 38 strains of mice. One genetic difference between high-activity strains (DBA/2 and C3H/He) and low-activity strains (C57BL/6 and C57BL/10) has been examined in detail. The phenotypic difference segregates as a single gene in both conventional crosses and between recombinant inbred (RI) strains. Immunoprecipitation has shown that the activity difference is due to an alteration in the number of enzyme molecules. The phenotypic difference between high and low strains can therefore be attributed to different alleles of a single regulatory locus, Hdc; the alleleHdc d determines low HDC concentration, and the allele Hdc d high concentration. Hdc has been mapped to chromosome 2 using data from both comparisons of strain distribution patterns of previously mapped loci within RI strains and a conventional three-point cross. The probable gene order is B2m-pa-Hdc, with map distances of 3.1±1.7 and 2.0±1.4 cM, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484230
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