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  • 1
    Electronic Resource
    Electronic Resource
    Cryo-electron microscopy of nervous tissue. A review
    Amsterdam : Elsevier
    Electron Microscopy Reviews 5 (1992), S. 341-380 
    Details
    ISSN: 0892-0354
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0892-0354(92)90015-I
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations in differentiating choroid plexus cells by puromycin
    Amsterdam : Elsevier
    Experimental Cell Research 74 (1972), S. 195-200 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(72)90497-1
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of 5-bromodeoxyuridine on the structure of differentiating choroid plexus cells in vitro
    Amsterdam : Elsevier
    Experimental Cell Research 78 (1973), S. 246-250 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(73)90065-7
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  • 4
    Electronic Resource
    Electronic Resource
    A quantitative freeze-etching study on the effects of cytochalasin B, colchicine and concanavalin A on the formation of intercellular contacts in vitro
    Amsterdam : Elsevier
    Experimental Cell Research 146 (1983), S. 361-369 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(83)90137-4
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  • 5
    Electronic Resource
    Electronic Resource
    Concanavalin A: Effects on the distribution of intramembranous particles and the reassembly of junctional contacts during the in vitro reaggregation of embryonic cells of nervous tissue
    Amsterdam : Elsevier
    Experimental Cell Research 123 (1979), S. 15-23 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(79)90417-8
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  • 6
    Electronic Resource
    Electronic Resource
    Effects of different perfluorochemicals on dorsal root ganglion cells in vitro (1998)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 236 (1998), S. 182-187 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  • Background: Investigation of the effects of different perfluorochemicals (PFC) on cultured dorsal root ganglion (DRG) cells. • Method: DRG cell cultures from 9- to 11-day-old chicken embryos were exposed to emulsified perfluorodecalin (PFD; C10F18; 0.5%, 1% and 10%) or perfluorooctylbromide (PFO; C8F17Br; 0.5%, 1% and 10%). The cells were evaluated under phase-contrast optics after 30 h and 120 h for 0.5 and 1% and after 5 h for 10%. To study the integrity of neuronal cells, immunohistochemical labelling for neurofilaments (NF) and tubulin (TUB) was performed. • Results: Concentrations of 0.5% and 1% of PFD or PFO did not change immunohistochemical labelling of DRG cells. Co-cultured macrophages showed a foam cell response, presumably representing ingested PFC. At both concentrations PFD induced a weaker foam cell response than PFO. A concentration of 10% led to the death of DRG cells and macrophages within 5 h. • Conclusion: PFC caused a dose-dependent damage of neuronal cells. Co-cultured macrophages developed a foam cell response similar to that observed in vivo after prolonged presence of PFC in the vitreous body. These observations indicate that PFD and PFO may not be suitable for long-term vitreous replacement in vitreoretinal surgery. However, the model is limited by several factors: (1) there are physiological differences between DRG cells and retinal ganglion cells; (2) in vivo retinal ganglion cells are protected by the overlying tissues; (3) the PFC used in tissue culture must be emulsified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004170050061
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  • 7
    Electronic Resource
    Electronic Resource
    Cyclic morphological changes of glial cells in long-term cultures of rat brain (1984)
    Springer
    Journal of neurocytology 13 (1984), S. 29-47 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Brain cells from embryonic rats were dissociated with trypsin, cultivated under constant conditions in Falcon flasks, and studied for periods of one year or more. Antisera against glial fibrillary acidic protein (GFA) and myelin basic protein (MBP) were used to identify glial cell types. For scanning electron microscopical (SEM) observation an embedding method in resin was developed that allows good preservation of the fine ultrastructural features of the cultivated cells and precise characterization of the cell types. Under our culture conditions, after four subcultures and 8–10 weeks of cultivation, the following cell types can be distinguished. (a) Flat epitheloid cells. From an immunocytological point of view these cells form a heterogeneous population composed of GFA- and MBP-positive and negative cells. They are the precursors of the following cell types. (b) Astroglial cells. SEM observations show a characteristic network of radially orientated prolongations. 92% of these cells are GFA-positive. (c) Oligodendroglial cells with characteristic dichotomously dividing branches. Secondary and tertiary branches end in flat amoeboid prolongations. These cells are MBP-positive. After approximately six weeks the most prominent cells are the flat epitheloid cells. The astroglial cells originate continuously from the epitheloid cells during the whole cultivation time. The formation of oligodendroglial cells, on the other hand, takes place at relatively precise intervals of time (approximately every 20–30 days) over the entire cultivation period (of more than one year).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01148317
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  • 8
    Electronic Resource
    Electronic Resource
    The formation of gap and tight junctions between retinal pigment cells in cell cultures (1979)
    Springer
    Journal of neurocytology 8 (1979), S. 229-238 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The reformation of the junctional complex of retinal pigment cells was studied after trypsin disaggregation andin vitro reaggregation. Control specimens show a zonula occludens (tight junction) with integrated gap junctions and very large macular gap junctions. Isolation after trypsination results in disaggregation of the large gap junctions and fragmentation of the tight junctions with disaggregation of their integrated gap junctions. After two to four days of incubation the restoration of the zonula occludens is complete. After approximately five days of incubation, large gap junctions are found with a patchy arrangement of particles similar to that seenin vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01175563
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  • 9
    Electronic Resource
    Electronic Resource
    Human cornea and sclera studied by atomic force microscopy (1997)
    Springer
    Cell & tissue research 288 (1997), S. 111-118 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Cornea ; Sclera ; Collagen ; Extracellular matrix ; Atomic force microscopy ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The purpose of this study was to investigate the ultrastructure of the extracellular matrix of human cornea and sclera by using the atomic force microscope (AFM). Specimens of human cornea (n=16) and sclera (n=10) were obtained from a cornea bank or from enucleated eyes (n=1; clinical and histopathological diagnosis: choroidal melanoma) and fixed in Karnovsky solution. The AFM resolved individual collagen fibrils in corneal and scleral tissue. Scleral collagen fibrils had a diameter ranging from 118.3 to 1268.0 nm and showed clear banding with a mean axial D-periodicity of 77.02 nm. The mean gap depth between the two overlaps was larger in the sclera than in the cornea. The diameter of corneal collagen fibrils ranged from 48.0 to 113.0 nm. In contrast to the sclera, the corneal collagen fibrils did not exhibit clear banding as their surface pattern. Closely attached fibrils with a beaded to globular structure were predominant in the cornea. The mean axial D-periodicity of the corneal collagen fibrils was 68.50 nm. In both tissues, the AFM resolved structures resembling cross-bridges between adjacent fibrils. The corneal collagen fibrils showed fibrillar properties that were different from those of the sclera, and that therefore might be essential for the spatial organization responsible for the optical quality of the cornea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050798
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  • 10
    Electronic Resource
    Electronic Resource
    The differentiation of neuroglia-müller-cells in the retina of chick (1965)
    Springer
    Cell & tissue research 66 (1965), S. 321-332 
    Details
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The developmental time sequence of Müller cells (MC) has been described. The maturation and proliferation of MC has a far reaching effect on the architecture of the whole retina, by forming the limiting membranes, and by separating synaptic complexes of receptors and bipolars. 2. The final stage of MC maturation reveals a polar distribution of organelles, the apical portion shows an accumulation of mitochondria, while the basal part contains fibrillary substance. 3. From the apical portion numerous microvilli protrude, which are the fibrillary baskets of light-microscopy. These microvilli contain many vacuoles, suggesting the uptake of substances. As fare as the basal portion of the MC is concerned pinocytosis can be demonstrated. 4. The possible role of MC as a universal glial cell of the retina has been discussed, for the MC appears to be the dominant type of neuroglia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334715
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